Immunohistochemical localization of estrogen receptors ERα and ERβ in the spiny mouse (Acomys cahirinus) ovary during postnatal development

Hułas-Stasiak, Monika; Gawron, Antoni

doi:10.1007/s10735-006-9072-3

Cited by 21 publications

(12 citation statements)

References 61 publications

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“…Both ESR subtypes are expressed in human and mouse oocytes and embryos [45][46][47]. To determine whether acute treatment with CC influences oocyte apoptosis and ESR subtype expression during OCC transport, we examined OCCs exposed to high-dose CC during the transport study for markers of apoptosis by Western blot analysis.…”

Section: Acute Treatment With CC Does Not Cause Apoptosis In Oocytes mentioning

confidence: 99%

Clomiphene Citrate Causes Aberrant Tubal Apoptosis and Estrogen Receptor Activation in Rat Fallopian Tube: Implications for Tubal Ectopic Pregnancy1

Shao

Nutu

Weijdegård

et al. 2009

Biology of Reproduction

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Clomiphene citrate (CC) therapy for disorders of anovulatory infertility has been linked to an increased frequency of tubal ectopic pregnancy. Although CC enhances apoptotic processes in the ovaries, villi, and decidual tissues, its effect on apoptosis in the fallopian tube is unknown. Here, we show that chronic treatment with CC induces tubal apoptosis, but not necrosis, through an intrinsic mitochondria-dependent signaling pathway in vivo. The apoptosis was specific to epithelial cells in the isthmus, and the damage was reversed with 17beta-estradiol (E2); however, pretreatment or concomitant treatment with E2 did not protect against tubal apoptosis induced by chronic treatment with CC. Chronic treatment activated estrogen receptors (ESRs), particularly cilia-localized ESR2A (formerly ERbeta2). In contrast to E2, acute treatment of superovulating rats with a high dose of CC or the ESR2-selective agonist 2,3-bis (4-hydroxyphenyl)-propionitrile (DPN) significantly delayed the transport of oocyte-cumulus complexes through the fallopian tube. Our findings suggest that in response to chronic CC therapy, isthmus-specific apoptosis of epithelial cells and activation of cilia-ESR2A act in parallel to block gamete and embryo passage through the fallopian tube, eventually resulting in tubal ectopic pregnancy.clomiphene citrate, ectopic pregnancy, estradiol receptor, estrogen receptor, fallopian tubes, intrinsic and extrinsic apoptotic pathways

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Section: Acute Treatment With CC Does Not Cause Apoptosis In Oocytes mentioning

confidence: 99%

Clomiphene Citrate Causes Aberrant Tubal Apoptosis and Estrogen Receptor Activation in Rat Fallopian Tube: Implications for Tubal Ectopic Pregnancy1

Shao

Nutu

Weijdegård

et al. 2009

Biology of Reproduction

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“…Hulas-Stasiak and Gawron [5] studied the ER-α and ER-β receptors immunohistochemically in spiny mouse ovaries and found the localisation of ER-α. Granulosa cells carried α receptors in all stages of developing follicle.…”

Section: Discussionmentioning

confidence: 99%

“…Follicle stimulation hormone (FSH) secretion is also very important in the regulation of gap junctions between the granulosa cells [3,4] . There are two specific estrogen receptors, so called as the estrogen receptor alpha (ER-α) and estrogen receptor β (ER-β), that are localised dispersedly within the ovarian tissue and responsible for the intraovarian movements [5,6] . Localisation and distribution of these two receptors given have been defined in immunohistochemical studies conducted in various animal species [7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

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Keçi Ovaryumunda Üreme Sezonu İçinde ve Dışında Östrojen Reseptör alfa ve Progesteron Reseptör B’nin Lokalizasyonu

Uslu¹,

Yörük²,

Mıs³

et al. 2016

Kafkas Univ Vet Fak Derg

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The main objective of this study was to investigate the localisation of estrogen receptor alpha (ER-α) and progesterone receptor B (PR-B) by immunohistochemistry in goat ovaries during in-and out of breeding season. The ovaries obtained from goats slaughtered in breeding season (n=10) and non-breeding season (n=10) were used. From the same animals, blood samples were taken to determine the levels of serum steroid hormones (E2, P4). The ER-α and PR-B immunohistochemical distributions within the ovaries were determined by the ABC method. In breeding season, the ER-α was detected in the germinal epithelium and follicular granulosa cells. The PR-B was determined to concentrate on the corpus luteum (CL) cells. The same receptors were also found to be weak in the theca externa cells of preovulatory follicles. In non-breeding season, the ER-α germinal epithelium and smooth muscle cells of certain blood vessels showed a weak positive reaction. The PR-B was positively stained in the germinal epithelium and few stroma cells. It was suggested that the average steroid hormone profiles in breeding (E2: 11.83±1.70 pg/mL, P4: 10.08±1.58 ng/ml) and non-breeding season (E2 2.33±0.85 pg/ml, P4 0.21±0.04 ng/ml) correlated well with the localisation intensity of receptors in goat ovaries. Keywords: Goat, ovary, immunohistochemistry, estrogen receptor α, progesterone receptor B Keçi Ovaryumunda Üreme Sezonu İçinde ve Dışında Östrojen Reseptör alfa ve Progesteron Reseptör B'nin Lokalizasyonu ÖzetSunulan bu çalışmada, üreme sezonu içinde ve dışındaki keçi ovaryumlarında, östrojen reseptör alfa (ER-α) ve progesteron reseptör B'nin (PR-B) yerleşim yerlerinin immünohistokimyasal olarak araştırılması amaçlandı. Mezbaha şartlarında kesilen dişi keçilerden, üreme sezonu içinde (n=10) ve dışında (n=10) elde edilen ovaryumlar kullanıldı. Aynı hayvanların, serum steroid hormon düzeylerini (E2, P4) belirlemek için kan örnekleri alındı. ER-α ve PR-B'nin ovaryumlardaki yerleşimleri immünohistokimyasal olarak ABC metodu ile belirlendi. Üreme sezonu içinde, ER-α reseptörleri germinatif epitelyum ve foliküllerin granüloza hücrelerinde saptandı. PR-B reseptörlerinin ise corpus luteum (CL) hücrelerinde yoğunlaştığı belirlendi. Aynı reseptörlerin, preovulator foliküllerin teka eksterna hücrelerinde de zayıf olarak bulundukları gözlendi. Üreme sezonu dışında, ER-α reseptörleri germinatif epitelyum ve bazı kan damarlarının düz kas hücrelerinde zayıf pozitif reaksiyon gösterdi. PR-B ise germinatif epitelyum ve bazı stroma hücrelerinde pozitif boyandı. Keçilerde, üreme sezonu içi-(E2: 11.83±1.70 pg/mL, P4: 10.08±1.58 ng/ mL) ve dışındaki (E2: 2.33±0.85 pg/ml, P4: 0.21±0.04 ng/ml) ortalama steroid hormon profillerinin bu reseptörlerin ovaryumlardaki lokalizasyon yoğunlukları ile uyumlu olduğu kanısına varıldı.

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“…These investigations were performed to describe the morphology of ganglionic structures and cholinergic and adrenergic nerve fibres supplying the pancreas in Egyptian spiny mouse (Acomys cahirinus, Desmarest) a species frequently used in medical and biological research (Scantlebury et al 2002, Szczurkowski et al 2002, Hułas-Stasiak and Gawron 2007. In addition, our results can be used in comparative anatomy analysis in the broader context of pancreas research in mammals.…”

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confidence: 99%

Autonomic Innervation of Pancreas in Egyptian Spiny Mouse (Acomys cahirinus, Desmarest)

et al. 2009

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The aim of the study was to obtain details of the morphology of the autonomic innervation of pancreas. Six adult Egyptian spiny mice (Acomys cahirinus, Desmarest) were studied for the presence and location of autonomic fibres and cells in the pancreas. The macromorphological investigations were performed using the thiocholine method adapted for this type of specimens. For processing tissues two histochemical techniques were used: thiocholine method on activity of AChE and the glyoxylic acid method for adrenergic structures.Cholinergic fibres and small autonomic ganglia were found among the secretory sections and along the pancreatic duct and both pancreaticoduodenal arteries, and its branches, reaching the Langerhans islets and forming around them a kind of net. From 24 to 40 AChE-positive ganglions in the whole exocrine part were observed. The highest density of cholinergic fibres was observed in the head of pancreas. Numerous adrenergic fibres that accompanied blood vessels as well as interlobular and intralobular ducts were found inside the exocrine parts of the pancreas. Neither adrenergic cells or adrenergic fibres were observed inside the pancreatic islets. Our results can be used in comparative anatomy studies of pancreas in mammals.

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Immunohistochemical localization of estrogen receptors ERα and ERβ in the spiny mouse (Acomys cahirinus) ovary during postnatal development

Cited by 21 publications

References 61 publications

Clomiphene Citrate Causes Aberrant Tubal Apoptosis and Estrogen Receptor Activation in Rat Fallopian Tube: Implications for Tubal Ectopic Pregnancy1

Clomiphene Citrate Causes Aberrant Tubal Apoptosis and Estrogen Receptor Activation in Rat Fallopian Tube: Implications for Tubal Ectopic Pregnancy1

Keçi Ovaryumunda Üreme Sezonu İçinde ve Dışında Östrojen Reseptör alfa ve Progesteron Reseptör B’nin Lokalizasyonu

Autonomic Innervation of Pancreas in Egyptian Spiny Mouse (Acomys cahirinus, Desmarest)

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