Immunohistochemical Studies of S‐100 Protein During Postnatal Ontogenesis of the Brain of Two Strains of Rats

Abstract

We have studied the dynamics of the appearance of cells reacting positively with anti-S-100 protein antiserum, during postnatal neurocytogenesis in the brain of rats of two strains differing in their susceptibility to sound stimuli. The postnatal time of appearance of cells reacting positively with anti-S-100 protein antiserum was somewhat later in rats susceptible to sound-induced seizures than in sound-resistant rats. These differences concerned mainly the cerebral cortex of 12-day-old rats. By day 21 of postnatal life these differences had disappeared. In subcortical structures of the brain, S-100 protein was first found on the 4th to the 5th day of life and the rate of appearance of cells containing this protein was similar in the two strains.

Specific and Artefactual Cellular Localizations of S100 Protein: An Astrocyte Marker in Rat Cerebellum

“…This label may be erroneously taken as a labelling of the neuronal plasma membrane. The observation of Sviridov et al [27] and Haglid et al [13] that neurones whose membrane is damaged give a positive cytoplasmic immunoreaction with anti-SlOO protein immune serum can be explained either as due to the penetration of the antibody in the neurone through the lesion in the membrane [13,27] or as the result of a simultaneous lesion in both plasma mem branes (of the neurone and its astrocytic wrapping) with subsequent leakage of SI00 protein from the astrocytic to the neuronal cytosol.…”

Dual Immunologic Property of S-100 Protein in Normal Eyes and Eyes with Retinoblastoma: A Histopathological and Immunohistochemical Study of 88 Cases

“…However, the immunostaining found only in glial cells in the human retina but not in neurons [7] allows it to be still used as glial marker for the histogenesis study of retino blastoma [2,3,11], However, the presence of the protein in neuronal cells including autono mic ganglia [8,9] and the substantial species variation in the retinal distribution of the pro tein [7] suggest that it is of limited value in investigating the same condition in chicken and human eyes. Molnar et al [10] found im munoreactivity of S-100 protein in the neuron of the chicken retina but not in human retina and indicated how misleading it can be to use the distribution of the protein in one species to generalize the distribution of the same protein in other species.…”

“…ical properties of S-100 protein in different cell types in the retina, because of the presence of the protein in the neurons inside and outside of the CNS [8,9] and the variation of the cell types expressing the protein in the retina of animals and human eyes [10,11],…”

Cellular and subcellular distribution of the S‐100 protein in rabbit and rat central nervous system

“…The S-100 protein (31) is present in and synthesized by glial cells (22,(2)(3)(4)9,27,12), but its location also in neurons seems by now undisputed (22,37,44,16,18,17). However, controversial views on the neuronal S-100 protein have been presented (33).…”