Immunohistochemical survey of pS2 expression in intraductal lesions associated with invasive ductal carcinoma of the breast

Inaji, Hideo; Koyama, Hiroki; Motomura, Kazuyoshi; Noguchi, Shinzaburo; Tsuji, Naoko; Wada, Akira

doi:10.1002/ijc.2910550602

Cited by 10 publications

(5 citation statements)

References 17 publications

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“…In the immu nohistochemical analysis, ER was detected only in nuclei and the expression of HSP27 was restricted to cytoplasm, suggesting that ER and HSP27 are not topologically asso ciated. Similar results were reported by others [8][9][10][11], though certain types of the HSPs, such as HSP90, had been demonstrated to be associated wtih steroid recep tors, mostly by immunoprécipitation assay [3]. In an in vitro assay, HSP27 proteins were induced by estrogen stimulation in estrogen-dependent cells [4], The physical association between the steroid receptor and HSP90 has been challenged recently as it might be attributable to arti facts due to the detergent [12], Thus it is likely that HSP27 is induced in the cytoplasm by stimulation of the ER, rather than by physiologic demands for binding with ER.…”

Section: Discussionsupporting

confidence: 81%

Immunohistochemical Detection of Estrogen Receptor in Invasive Human Breast Cancer: Correlation with Heat Shock Proteins, pS2 and Oncogene Products

et al. 1995

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The authors immunohistochemically studied the expression of the estrogen receptor (ER), 27-kD heat shock protein (HSP27) and pS2 in 118 invasive primary human breast cancers. Positive nuclear staining of the ER was detected in 64% of the cases and was closely correlated wtih the biochemical assay (p < 0.0001). ER-positive tumors were significantly decreased with tumor size and stage (p < 0.001 each), but not with lymph node status. Positiv-ity of the ER was correlated with the cytoplasmic expression of HSP27 (p < 0.005), pS2 (not significant) and HSP70 (not significant). ER negativity was significantly correlated with the expression of p53, epidermal growth factor receptor (EGFR) and c-erbB-2 (p < 0.05 each). Thus, it was concluded that ER-positive breast carcinomas, relatively small in size, preferentially expressed HSP27, HSP70 and pS2 and that ER-negative tumors, relatively large in size, were predisposed to express p53, EGFR and c-erbB-2.

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Section: Discussionsupporting

confidence: 81%

Immunohistochemical Detection of Estrogen Receptor in Invasive Human Breast Cancer: Correlation with Heat Shock Proteins, pS2 and Oncogene Products

et al. 1995

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“…In addition to cells, concentrations of various proteins, hormones, and lipids in NAF have also been associated with breast cancer risk (4)(5)(6). Potential tumor markers, including carcinoembryonic antigen, prostate specific antigen, c-erbB-2 (Her2/neu), basic fibroblastic growth factor, and vascular endothelial growth factor have been detected in NAF using ELISA and/or western blot (7)(8)(9)(10). Recent advances in proteomic technologies, especially in mass spectrometry, have enabled further characterization of proteomes of NAF and identification of markers associated with breast cancer risk, such as α1-acid glycoprotein and vitamin D binding protein (4,11,12).…”

Section: Introductionmentioning

confidence: 99%

Dietary Intake of Lactose as a Strong Predictor for Secretor Status of Nipple Aspirate Fluid in Healthy Premenopausal Nonlactating Women

Huang

Anderson

Nagamani

et al. 2008

Clinical Cancer Research

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Purpose-Nipple aspirate fluid (NAF) is considered a potential source for discovering breast cancer biomarkers. However, the success rate of obtaining NAF was reported to vary from 48 to 77%, and mechanisms for its secretion are not fully understood. The purpose of this study was to investigate dietary, demographic, reproductive, hormonal, and anthropometric factors that are associated with the ability to obtain NAF by aspiration (secretor status) from premenopausal women.Study Design-NAF procedures were attempted for women who were 30-40 years old, not pregnant, not breastfeeding, and not taking contraceptive medications.Results-Compared with non-secretors, secretors of NAF consumed significantly more dietary lactose (mainly from milk), were more likely to be parous, were older at first and last childbirth, breastfed their babies for a longer period of time, and had an earlier menarche and lower plasma concentrations of 17β-estradiol (P<0.05). Using multivariate logistic regression models, higher dietary intake of lactose [Odds Ratio (OR) =2.7; 95% Confidence Interval (CI): 1.5-4.8], earlier menarche (OR=0.8, CI: 0.7-1.0), being parous (OR=2.3, CI: 1.0-5.6), and older at first childbirth (OR=1.5, CI: 1.0-2.1) were found to be independent and positive predictors for being a secretor of NAF.Conclusions-These findings suggest that dietary intake of lactose, a modifiable factor, may be used to change the NAF secretor status of women. This finding may facilitate the use of NAF as a diagnostic material for detecting breast diseases.

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“…The method of pS2 staining was described in detail elsewhere [16]. Briefly, formalin-fixed paraffin-embedded tissue samples were cut into 4 pm thick sections.…”

Section: Ps2 Stainingmentioning

confidence: 99%

Effect of Tamoxifen on pS2 Expression in Human Breast Cancers

Motomura¹,

Koyama²,

Noguchi³

et al. 1997

Oncology

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pS2 protein expression is induced by estrogen through estrogen receptors (ER), and is known to be inhibited by antiestrogen in the breast cancer cell line MCF-7. The present study was undertaken to determine whether pS2 expression may be inhibited by the antiestrogen tamoxifen (TAM) human breast cancer. pS2 expression was immunohistochemically investigated in 22 patients treated preoperatively with TAM and in 45 without TAM treatment (control group). Immunostaining was considered positive when 10% of tumor cells showed cytoplasmic staining. We found that 94% of the pS2-positive tumors were ER-positive (ER+), and there was a significant association between the two proteins (p < 0.0001). In ER+ tumors, pS2 expression was seen in only 21% (3 of 14) of the TAM+ group, compared to 70% (16 of 23) of the control group. Statistical analysis of these data indicated a high likelihood (p = 0.006) of TAM treatment being associated with inhibition of pS2 expression in human breast cancers.

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Immunohistochemical survey of pS2 expression in intraductal lesions associated with invasive ductal carcinoma of the breast

Cited by 10 publications

References 17 publications

Immunohistochemical Detection of Estrogen Receptor in Invasive Human Breast Cancer: Correlation with Heat Shock Proteins, pS2 and Oncogene Products

Immunohistochemical Detection of Estrogen Receptor in Invasive Human Breast Cancer: Correlation with Heat Shock Proteins, pS2 and Oncogene Products

Dietary Intake of Lactose as a Strong Predictor for Secretor Status of Nipple Aspirate Fluid in Healthy Premenopausal Nonlactating Women

Effect of Tamoxifen on pS2 Expression in Human Breast Cancers

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