Immunohistochemistry in diagnostic ophthalmic pathology: a review

Eagle, Ralph C.

doi:10.1111/j.1442-9071.2008.01870.x

Cited by 15 publications

(10 citation statements)

References 61 publications

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“…Due to similar embryologic origin, malignant melanoma and schwannoma express several identical proteins; however, positive staining with the immunohistochemical marker Melan A is more supportive of melanoma . A diagnosis of PNST was supported in the present case due to negative immunohistochemical staining with anti‐Melan A, GFAP, neurofilament and PNL2, and positive staining with S100 and NSE . Fontana‐Masson confirmed the presence of melanin pigment.…”

Section: Discussionsupporting

confidence: 57%

Retrobulbar pigmented peripheral nerve sheath tumor in a dog

Curto

Clode

Durrant

et al. 2015

Veterinary Ophthalmology

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A 1-year-old male castrated Pug was referred for unilateral exophthalmos unresponsive to oral antibiotic and anti-inflammatory therapy. Clinical findings included exophthalmos of the left eye with lateral strabismus, resistance to retropulsion, and an elevated nictitans. Hematologic and biochemical analyses were within normal limits. Findings following computed tomography (CT) of the head included an expansile retrobulbar soft tissue mass with bony lysis extending into the left nasal cavity and nasopharynx. Ultrasound-guided fine-needle aspirates and biopsy samples obtained via rhinoscopy were nondiagnostic. Palliative exenteration was elected; the patient was euthanized 13 weeks following exenteration due to development of neurologic signs and perceived poor quality of life. The histopathologic diagnosis was a malignant pigmented peripheral nerve sheath tumor.

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Section: Discussionsupporting

confidence: 57%

Retrobulbar pigmented peripheral nerve sheath tumor in a dog

Curto

Clode

Durrant

et al. 2015

Veterinary Ophthalmology

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“…29 Childhood lesions, in which the junctional component and an inflammatory infiltrate are often pronounced, can be disquieting. Therefore, our findings regarding HMB-45 and Ki-67 immunostaining should provide an additional level of confidence in separating benign from malignant conditions.…”

Section: Commentmentioning

confidence: 99%

Immunohistochemical Studies of Conjunctival Nevi and Melanomas

Jakobiec

Bhat²,

Colby³

2010

Arch Ophthalmol

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To evaluate the role of immunohistochemical methods in the diagnosis of benign and malignant conjunctival melanocytic proliferations.Design: Retrospective immunohistopathologic study.Methods: Paraffin-embedded tissue sections from 20 conjunctival nevi and 15 invasive melanomas were immunoreacted with antibodies against cellular antigens S-100 protein, MART-1, HMB-45, CD-45, and Ki-67 nuclear proliferation protein.Results: All nevi immunostained moderately to strongly for S-100 protein and MART-1. Results for HMB-45 were negative in the middle and lower subepithelial portions of 18 of 20 lesions; it was usually only weakly positive within the superficial junctional zone. Only 1 melanoma did not stain positively for S-100; MART-1 and HMB-45 were positive in all lesions at some level of intensity. Ki-67 positivity was restricted to the junctional zone of nevi and was diffuse in melanomas. The mean Ki-67 proliferation indices were 1.89% for the nevi and 17.3% for the melanomas. CD-45 can help to highlight lymphocytes that immunostain with Ki-67. Melanomas in situ and atypical primary acquired melanoses had more than twice the Ki-67 proliferation counts of intraepithelial junctional nevocytes (P Ͻ .001) and more intense HMB-45 cytoplasmic staining than junctional zone nevocytes.Conclusions: S-100 and MART-1 were not useful in separating benign from malignant lesions. Results for nevus cells beneath the junctional zone were overwhelmingly negative for HMB-45 and Ki-67. Two nevi and all melanomatous nodules were positive for HMB-45 (PϽ.001). A higher Ki-67 proliferation index convincingly separated melanomas from nevi (P Ͻ .001). Immunostaining for HMB-45 and Ki-67 are valuable adjuncts to careful histopathologic evaluation in assessing benign and malignant conjunctival melanocytic tumors.

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“…Cytokeratin 3 ⁄ 12 (CK 3 ⁄ 12) was detected with a monoclonal anti-mouse antibody (K3 ⁄ 12, 1:100; Progen Biotechnik, Heidelberg, Germany). Conventional immunohistochemistry methods (Bancroft & Gamble 2007) were applied with Alexa Fluor 555 goat anti-mouse antibody (1:500; Molecular Probes) and Alexa 633 goat anti-rabbit antibody (1:500; Molecular Probes) as secondary antibodies (Eagle 2008). After counterstaining with Hemalum Ò (Merck), the 5-to 7-lm sections were finally mounted in aqueous medium (Aquatex; Roche Applied Science, Basel, Switzerland).…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Bone marrow cells and CD117‐positive haematopoietic stem cells promote corneal wound healing

Sel

Schilling

Naß

et al. 2012

Acta Ophthalmologica

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ABSTRACT.Purpose: The present study was conducted to evaluate the therapeutic effects of topically applied bone marrow (BM) cells and CD117-positive haematopoietic stem (CD117 + ) cells on alkali-induced corneal ulcers. Methods: Bone marrow cells and CD117 + cells were isolated from syngenic mice and labelled with an intracellular cell tracer. Defined corneal wounds were produced in 89 eyes of syngenic mice and allowed to partially heal in vivo for 6 hr. The alkali-burned eyes were enucleated 6 hr postinjury and randomly divided into three groups. Control group (33 eyes) was incubated with medium only. The treatment groups received either BM cells (30 eyes) or CD117 + cells (26 eyes) suspended in medium. Re-epithelialization process of corneal defects was qualitatively and quantitatively assessed and statistically analysed. The corneas were examined by histological and immunohistochemical methods. Results: We found that the re-epithelialization of corneal wounds in both treatment groups was significantly accelerated as compared to the control group. During the follow-up period (85 hr), the corneal transparency was comparable in all groups. Morphological investigations of corneas from control and treatment group showed no evident differences in the phenotype of the regenerated epithelium. Additionally, corneas in the treatment groups were devoid of donor-derived BM cells and CD117 + cells, respectively. Conclusions: This study provides evidence that topical application of BM cells or CD117 + cells can be used to reconstruct corneal surfaces. Because neither BM cells nor CD117 + cells were integrated into the corneal epithelium, we suggest that soluble factors could be responsible for the positive effect of BM cells and CD117 + cells on corneal wound healing.

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Immunohistochemistry in diagnostic ophthalmic pathology: a review

Cited by 15 publications

References 61 publications

Retrobulbar pigmented peripheral nerve sheath tumor in a dog

Retrobulbar pigmented peripheral nerve sheath tumor in a dog

Immunohistochemical Studies of Conjunctival Nevi and Melanomas

Bone marrow cells and CD117‐positive haematopoietic stem cells promote corneal wound healing

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