Immunohistological Study of Wound Healing after Submucosal Radiofrequency Tissue Ablation of Inferior Nasal Turbinate in a Sheep Model

Gouveris, Haralampos; Nousia, Christina; Giatromanolaki, Alexandra; Riga, Maria; Katotomichelakis, Michael; Papathanasiou, Jannis; Watelet, Jean-Baptiste; Danielides, Vassilios

doi:10.2500/ajra.2011.25.3576

Cited by 12 publications

(15 citation statements)

References 21 publications

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“…The daily clinical usage of gadolinium (Gd) based contrast agents in magnetic resonance imaging is limited because of its nephrogenic toxicity 25. Fluorescent dye-labeled hMSCs have a relatively low penetration capacity, and are therefore not effective for clinical use 26. In vivo tracking of hMSCs-UC has still been a technical barrier to the use of hMSCs in research and clinical investigations.…”

Section: Discussionmentioning

confidence: 99%

Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats

et al. 2014

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The aim of this study is to explore the localization of human mesenchymal stem cells from umbilical cord matrix (hMSCs-UC) and the role of these cells in the repair of foot ulcerate tissue in diabetic foot ulcers in rats. A diabetic rat model was established by administering Streptozotocin. Diabetic foot ulceration was defined as non-healing or delayed-healing of empyrosis on the dorsal hind foot after 14 weeks. hMSCs-UC were delivered through the left femoral artery. We evaluated the localization of hMSCs-UC and their role in tissue repair in diabetic foot ulcers by histological analysis, PCR, and immunohistochemical staining. A model for diabetes was established in 54 out of 60 rats (90% success rate) and 27 of these rats were treated with hMSCs-UC. The area of ulceration was significantly and progressively reduced at 7 and 14 days following treatment with hMSCs-UC. This gross observation was strongly supported by the histological changes, including newly developed blood vessels and proliferation of inflammatory cells at 3 days post-treatment, significant increase in granulation tissue at 7 days post-treatment and squamous epithelium or stratified squamous epithelium at 14 days post-treatment. Importantly, human leukocyte antigen type-I (HLA-1) was confirmed in ulcerated tissue by RT-PCR. The expression of cytokeratin 19 was significantly increased in diabetic model rats, with no detectable change in cytokeratin 10. Additionally, both collagens I and III increased in model rats treated with hMSCs-UC, but the ratio of collagen I/III was less significant in treated rats compared with control rats. These results suggest that hMSCs-UC specifically localize to the target ulcerated tissue and may promote the epithelialization of ulcerated tissue by stimulating the release of cytokeratin 19 from keratinocytes and extracellular matrix formation.

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Section: Discussionmentioning

confidence: 99%

Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats

et al. 2014

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“…(MMP9) after application of RTA in sheep turbinates have been presented (4) . In the present report, we present a wound healing successfully (8) .…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical studies of wound healing after monopolar electrocautery and ultrasound submucosal inferior nasal turbinate reduction in sheep

Nousia¹,

Gouveris²,

Giatromanolaki³

et al. 2013

Rhin

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“…Animal models are useful not only for elucidation of the complex mechanisms of wound healing but also for the development of novel therapeutics enhancing healing. Some animal models of nasal wound healing are available, but most of them are large animals such as sheep and rabbits, presumably because of the technical problems associated with manipulation of small nostrils . A small animal model would be very useful because large numbers of animals are readily available and novel potential therapeutics can be topically administered without sedation or anesthesia, unlike the case in larger animals.…”

Section: Introductionmentioning

confidence: 99%

“…Some animal models of nasal wound healing are available, but most of them are large animals such as sheep and rabbits, presumably because of the technical problems associated with manipulation of small nostrils. 9,10 A small animal model would be very useful because large numbers of animals are readily available and novel potential therapeutics can be topically administered without sedation or anesthesia, unlike the case in larger animals. Here, we developed a mouse model of nasal wound healing after mechanical injury and investigated the wound-healing process.…”

Section: Introductionmentioning

confidence: 99%

Establishment and verification of a mouse model of nasal wound healing

Joo

Jeon

et al. 2019

The Laryngoscope

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Objectives Poor wound healing as reflected by the development of synechia and osteitis after endoscopic sinus surgery may trigger disease recurrence. Animal models provide insights into the pathogenesis of poor wound healing and may aid in the development of new therapeutics. Here, we established a mouse model of nasal wound healing and confirmed its utility. Study Design Animal study. Methods Unilateral intranasal wounds were induced using a small interdental brush in 6‐week‐old C57BL/6 mice. Forty‐five mice were divided into three groups (each n = 15): one control and two experimental groups (intranasal vs. intraperitoneal dexamethasone). Mice were sacrificed on days 2, 14, and 28 after injury (each n = 5). Serial changes in nasal wound histopathology were described, and intergroup differences were analyzed. Results On day 2, mucosal detachment, hemorrhage, and exudate were observed. On day 14, synechiae featuring neo‐osteogenesis (bone lacunae, osteoblasts, and multinucleated osteoclasts) between the septum and the maxilloturbinate were prominent, followed by wound maturation on day 28: fewer lacunae and smaller osteoblasts. Macrophages were evident only on day 2, and lymphocytes were predominant on day 28. The amount of exudate on day 2 and the synechial area on day 28 were significantly reduced in mice that received dexamethasone systemically compared with control mice, with similar trends in those treated intranasally. Conclusion Our mouse model of nasal wound healing was characterized by the development of bony synechia and neo‐osteogenesis, not soft‐tissue synechia. The model may be useful in the assessment of novel therapeutics to prevent those wounds. Level of Evidence NALaryngoscope, 129:E266–E271, 2019

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Immunohistological Study of Wound Healing after Submucosal Radiofrequency Tissue Ablation of Inferior Nasal Turbinate in a Sheep Model

Abstract: Given their high tissue concentrations after RTA application, fibronectin, collagen III, CD68, and MMP-9 deserve further study as candidate modulators of the INT wound-healing process.

Cited by 12 publications

References 21 publications

Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats

Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats

Immunohistochemical studies of wound healing after monopolar electrocautery and ultrasound submucosal inferior nasal turbinate reduction in sheep

Establishment and verification of a mouse model of nasal wound healing

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