Immunological Properties of “Bombay” Phenotype

Iseki, Shoei; Takizawa, Hisao; Takizawa, Haruko

doi:10.2183/pjab1945.46.803

Cited by 7 publications

(2 citation statements)

References 5 publications

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“…These inhibiting patterns seem to be similar to those observed with eel serum and Lotus lectin (Watkins & Morgan, 1962), both of which are specific for fucose linked 1-*-2 to galactose (i.e., H-determinant structure). The following sugars failed to give significant inhibition when tested up to the concentrations (mM) indicated; Glc, 100; Gal, 100; Man, 50; rhamnose, 50; GlcNAc, 50; GalNAc, 50; lactose, 50; LNT,25. It should be emphasized that A. aurantia lectin was able to agglutinate Bombay phenotype cells, Oh Le(a+b-) (Iseki et al, 1970), although the reaction was weaker than with group O cells. The Oh cell lacks the H-determinant structure and fails to react with anti-H reagents.…”

Section: Resultsmentioning

confidence: 92%

Purification and properties of a novel fucose-specific hemagglutinin of Aleuria aurantia

Kochibe¹,

Furukawa²

1980

Biochemistry

180

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A fucose-binding lectin from fruiting bodies of Aleuria aurantia was purified by affinity chromatography by using the H-active glycopeptide of desialyzed porcine submaxillary mucine coupled to Sepharose 4B and eluting with L-fucose. Homogeneity of the active protein was confirmed by polyacrylamide gel electrophoresis, isoelectric focusing, column chromatography using Sephadex G-100, and ultracentrifugal analyses. It has a molecular weight of 72 000 and is proposed to be a dimer of identical subunits, each of which has combining site of uniform affinity. Chemical analyses revealed the absence of the sulfur-containing amino acid and carbohydrate, neutral and amino sugar, in the lectin molecule. It agglutinated human erythrocytes of all ABO and Lewis types, Bombay phenotype, and group O cells treated with alpha (1 leads to 2)-fucosidase. In double-diffusion experiments, the lectin formed a single precipitin line which fused with all of the fucose-containing blood-group substances tested, including the alpha-fucosidase-treated materials. These findings together with the results of hemagglutination and precipitation studies indicate that the lectin combines the terminal fucose in the carbohydrate chain but doses not require a particular linkage to the penultimate sugar moiety.

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Section: Resultsmentioning

confidence: 92%

Purification and properties of a novel fucose-specific hemagglutinin of Aleuria aurantia

Kochibe¹,

Furukawa²

1980

Biochemistry

180

View full text Add to dashboard Cite

show abstract

“…Human O, A1; A2, B, AB and Oh red cells were used. Oh red cells, frozen in glycerol were supplied by the Department of Legal Medicine, School of Medicine, Gunma University [11], [12,13] was cultured in ordinary agar at 37 °C for 24 h, the cells were suspended in saline solution, and the suspension was incubated at 37 °C for 24 h with frequent shaking to disrupt them. The solution was centrifuged at a high speed, and the fraction obtained from the super natant at 30-60% ammonium sulfate saturation was used as a crude preparation of Hdecomposing enzyme.…”

Section: Methodsmentioning

confidence: 99%