Immunological studies of women with primary breast carcinoma

Jeejeebhoy, H. F.

doi:10.1002/ijc.2910150602

Cited by 15 publications

(12 citation statements)

References 16 publications

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“…Cytotoxicity tests.-A modification of a previously described method (Takasugi and Klein, 1970) (Jeejeebhoy, 1975), using the procedures described in this paper, have not as yet demonstrated any prozone phenomena but these do remain real possibilities. Nor have these studies shown a sequence similar to that found in mouse systems, where opposite effects occasionally result when sera are diluted (Skurzak et al, 1972).…”

Section: Methodsmentioning

confidence: 88%

“…Lymphoid cell8.-Previous publications have described the solutions used in this study (Jeejeebhoy, 1974) and the procedures employed for separation of lymphoid cells on a Ficoll-Hypaque gradient and their subsequent freezing (Jeejeebhoy, 1975 (Ozzello et al, 1974). We froze it in aliquots at subculture generation 282.…”

Section: Methodsmentioning

confidence: 99%

“…Cell suspensions from these were prepared by mechanical dissociation as previously described (Jeejeebhoy, 1975). On some occasions (C20-26) an attempt was made to increase the number of viable tumour cells by utilizing only the interface which remained when the initial tumour cell suspension was washed and then centrifuged on FicollHypaque (sp.…”

Section: Methodsmentioning

confidence: 99%

“…This controversy stems from the fact that lymphoid cells from apparently disease-free individuals are often cytotoxic to tumour cells in vitro (Takasugi, Mickey and Terasaki, 1973;Jeejeebhoy, 1975;Berkelhammer et al, 1975) and that sera from these same individuals can often decrease (" block " in the current terminology) or potentiate these effects by agents which seem to be immunologically specific for the tumour cells (Jeejeebhoy, 1975 Tables I and II were comparable (differences of 20% and less are rarely statistically significant in this type of in vitro cytotoxicity assay). This suggests that the results obtained represented real phenomena and were not fortuitous and unrepeatable observations.…”

Section: Controlsmentioning

confidence: 99%

“…All these facts, taken together, suggest that at least some of the cells in these 2 lines were of malignant origin. BT20 is a cell line derived from a breast adenocarcinoma (Lasfargues and Ozzello, 1958) and it forms progressively growing tumours in athymic nude mice (Ozzello et al, 1974 (Jeejeebhoy, 1975) (Hellstrom and Hellstrom, 1974) or lymphoid cells (Currie andBasham, 1972) for antibody or antigen-antibody complexes, quantitative differences in the amounts of free antibody, free antigen and/or free antigen-antibody complexes, etc. No consistent pattern of target-cell susceptibility to humoral effects was noted.…”

Section: Controlsmentioning

confidence: 99%

See 4 more Smart Citations

Localization by immunoperoxidase and estimation by radioimmunoassay of carcinoembryonic antigen in colonic polyps

Sharkey¹,

Hagihara²,

Goldenberg³

1977

Br J Cancer

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A 3-layer immunoperoxidase technique was used to demonstrate carcinoembryonic antigen (CEA) in colonic polyps from patients with or without previous or concurrent malignancy. CEA was demonstrated in a higher percentage of the polyps received as fresh specimens that were rapidly frozen and fixed in ethanol, than in formalin-fixed, paraffin-embedded sections. Tissue CEA content of both colonic carcinomas and polyps was determined by radioimmunoassay, and it was found that benign colonic tumours had levels of tissue CEA comparable to colonic cancer, indicating that CEA concentration in a tumour does not reflect its grade of malignancy. In fact, in one case in which both colonic cancer and polyps were removed, the polyps had the higher quantities of tissue CEA. Further, tissue CEA concentration of a polyp was not dependent on its size or location. Studying the titres of circulating CEA in these patients revealed an elevation of plasma CEA in one-third of the patients with only colonic polyps, whilst the patients with cancer all had increased titres. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6

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Section: Methodsmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Controlsmentioning

confidence: 99%

Section: Controlsmentioning

confidence: 99%

See 3 more Smart Citations

Localization by immunoperoxidase and estimation by radioimmunoassay of carcinoembryonic antigen in colonic polyps

Sharkey¹,

Hagihara²,

Goldenberg³

1977

Br J Cancer

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Changes in cell-mediated immunity in patients undergoing radiotherapy

Rafla¹,

Yang²,

Meleka³

1978

Cancer

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The cell-mediated immune status of 147 patients who received radiotherapy was evaluated using zn vitro tests (PHA, E-rosette and spontaneous blastogenesis) both before and 6 weeks after the end of radiation. All patients had verified malignancies, involving the bronchus in 29 cases, breast in 28, female genital system in 26, head and neck in 20 and bladder in 15. Patients suffering from bronchogenic carcinomas or malignancies of the head and neck showed a relative high degree of immune suppression. O u r findings indicate a trend towards some improvement in PHA reactivity, as well in as the percentage of E-rosette-forming cells after treatment, which is more noticeable in patients with pelvic or breast tumors. A relationship seems to exist between the tumor load and the immune status, which reverts to a normal pattern when the former is extinguished. Moreover, patients with poor clinical response display a profoundly depressed level of immune status without any improvement after treatment.

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Sequential in vitro reactivity of lymphocytes from melanoma patients receiving immunotherapy compared with the reactivity of lymphocytes from healthy donors

Berkelhammer

Mastrangelo

Laucius

et al. 1975

Intl Journal of Cancer

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A microcytotoxicity technique was used to determine the sequential in vitro reactivity against melanoma cells of lymphocytes from melanoma patients receiving immunotherapy and from healthy donors. Lymphocytes were collected 2 weeks for 2-3 months and were stored in liquid nitrogen until use. Preliminary studies had indicated that freezing did not effect the reactivity of lymphocytes. Lymphocytes from 10 healthy donors tested against melanoma cells exhibited substantial reactivity which showed no consistent pattern over time. Lymphocytes from 9 melanoma patients exhibited increased reactivity after immunotherapy. Patterns of reactivity against melanoma cells and against bladder carcinoma cells were similar, indicating lack of specificity for melanoma antigens. Correlations with clinical course of the disease were not apparent.

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Immunological studies of women with primary breast carcinoma

Cited by 15 publications

References 16 publications

Localization by immunoperoxidase and estimation by radioimmunoassay of carcinoembryonic antigen in colonic polyps

Localization by immunoperoxidase and estimation by radioimmunoassay of carcinoembryonic antigen in colonic polyps

Changes in cell-mediated immunity in patients undergoing radiotherapy

Sequential in vitro reactivity of lymphocytes from melanoma patients receiving immunotherapy compared with the reactivity of lymphocytes from healthy donors

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