Immunomodulatory Effects of Polysaccharides Derived from Persimmon Leaves on Cyclophosphamide-induced Immunosuppressed Mice

Abstract

We prepared 2 different crude polysaccharides from persimmon leaves, by hot water (PLW-0) and pectinase digestion (PLE-0), respectively. PLW-0 and PLE-0 showed similar sugar compositions and contained 11 different sugars, including rarely observed sugars such as 2-methyl-fucose, 2-methyl-xylose, apiose, and aceric acid. However, the uronic acid content of PLE-0 was lower than that of PLW-0, because of pectinase treatment. In normal mice, administration of PLW-0 and PLE-0 increased the spleen index and splenocyte proliferation. The effect of PLE-0 on the spleen index and splenocyte proliferation was greater than that of PLW-0. We subsequently assessed the immunomodulatory activities of PLW-0 and PLE-0 on cyclophosphamide (CY)-induced immunosuppressed mice. We revealed that mice treated with PLW-0 or PLE-0 showed increased splenocyte proliferation, NK cell activity, and white blood cell numbers. Taken together, our results indicate that PLW-0 and PLE-0 can enhance immune function in normal mice and modulate CY-induced immune suppression.

Preventive Effect of the Herbal Preparation, HemoHIM, on Cisplatin-Induced Immune Suppression

“…However, reduced CD4+ T lymphocyte ratio by cisplatin in splenocytes was significantly restored by HemoHIM treatment similar to the findings of Lee et al [40]. Previous studies showed that immunosuppressive reagents inhibited the proliferation of lymphocytes and splenocytes [41, 42]. The present study showed that cisplatin inhibited splenocyte proliferation, but HemoHIM was restored in a concentration-dependent manner.…”

Immunostimulatory polysaccharide isolated from the leaves of Diospyros kaki Thumb modulate macrophage via TLR2

“…PLE0 was kindly provided by Hee-Do Hong (Korea Food Research Institute) and its preparation was described previously [20]. Briefly, leaves of dried D. kaki Thumb (100 g) were suspended in 2 L of distilled water (adjusted to pH 5.0 with HCl) and hydrolyzed with pectinase (1 mL) for 3 days at 50 • C. Pectinase was then deactivated by boiling for 20 min at 100 • C. Enzyme-treated persimmon leave was centrifuged at 6500 × g for 20 min to obtain the supernatant.…”

DEU-7 Derived from Ulmus macrocarpa Improved Immune Functions in Cyclophosphamide-treated Mice

“…It has been known to reduce the production of lymphocytes in the spleen and lymph node. The appropriate treatment concentration of cyclophosphamide was determined based on the various experimental studies [4,7,12,16]. An in vivo test was performed by feeding the mice DEU-2, 3, 4 and 7 once a day.…”