Immunoperoxidase studies by monoclonal antibody B72.3 applied to breast aspirates: Diagnostic considerations

Lundy, Joel; Kline, Tilde S.; Lozowski, Mary; Chao, Sylvia

doi:10.1002/dc.2840040203

Cited by 11 publications

(6 citation statements)

References 7 publications

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“…Despite these promising reports, the use of MAB B72.3 as an aid in differentiation between atypical hyperplasia and carcinoma has remained limited. This is mainly due to scattered reports of false-positive immunostaining seen in fibrocystic changes and apocrine cells (48). We believe that, morphologically, the usual pattern of fibrocystic change and apocrine cells are recognizable.…”

Section: Immunostainingmentioning

confidence: 84%

Cytomorphology of Fibrocystic Change, High-Risk, and Premalignant Breast Lesions

Masood

1995

Breast Journal

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Fine-needle aspiration biopsy has proven t o be an accurate and well-tolerated procedure with a reported specificity of 99% and sensitivity of 70-99%. Similarly, nonpalpable breast lesions can be effectively sampled by needle biopsies under radiologic guidance. Breast aspirates have also been extensively utilized for assessment of nuclear grade, hormone receptor status, ploidy status, and proliferation rate, and have proven to be an attractive alternative to surgical biopsy. Using strict cytologic criteria, it may also be possible to recognize the cytomorphologic changes in breast lesions that are associated with increased risk for subsequent development of breast cancer. Presence of myoepithelial cells within the clusters of atypical epithelial cells recognized morphologically andlor detected by immunostaining for muscle specific actin is an important diagnostic feature of proliferative breast disease with atypia (atypical hyperplasia). This recognition has significant clinical implications and is important in the design of chemoprevention trials. The cytologic distinction between carcinoma in situ and invasive breast cancer remains difficult. rn ibrocystic change, the most commonly diagnosed be-F nign breast disease, reflects a spectrum of changes that range from normal physiologic alterations in the breast to proliferative changes approximating in situ carcinoma. These include cyst formation, apocrine metaplasia, stroma1 fibrosis, and various degrees of ductal hyperplasia.It has been known for many years that women who have undergone a breast biopsy for so-called "fibrocystic dis-

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Section: Immunostainingmentioning

confidence: 84%

Cytomorphology of Fibrocystic Change, High-Risk, and Premalignant Breast Lesions

Masood

1995

Breast Journal

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“…The antibody was reactive to three of four infiltrating lobular cancers. In a recent study nine of 10 additional cases of infiltrating lobular cancer were reactive to MAb B72.3 [15]. In the two cases in which a diagnosis was not established by the cytopathologist, one was reactive and one nonreactive to the antibody.…”

Section: Discussionmentioning

confidence: 95%

Immunocytochemistry with monoclonal antibody B72.3 applied to a spectrum of benign and malignant breast aspirates — strengths and pitfalls

Lundy

Lozowski

Mishriki

1988

Breast Cancer Res Tr

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Fine needle aspiration cytology (FNA) was performed on 168 breast masses. In all cases aspirates were reacted with monoclonal antibody (MAb) B72.3 using the ABC immunoperoxidase technique. MAb B72.3 demonstrated reactivity in nine of 90 malignant cases in which the cytologic diagnosis was not positive. In eight benign cases in which the cytopathologist made a suspicious diagnosis, MAb B72.3 demonstrated cytoplasmic reactivity in only one case. In three cytologically benign cases MAb B72.3 reacted strongly positive. The overall diagnostic accuracy of this immunoperoxidase technique was approximately 95 percent. In about five to 10 percent of cases, monoclonal antibody B72.3 can be a valuable diagnostic adjunct to routine cytopathologic diagnosis. False positives present pitfalls that must be considered.

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“…Application of molecular techniques to cell samples is an adjunct to morphology that can be of assistance to the cytologist. Immunohistochemistry, 13,17 flow cytometry, 10,16,19,22 in situ hybridization, 11 fluorescence in situ hybridization 21 and PCR-based methods 4,7 have been applied to cytologic samples. In a recent study the utility of detecting expression of carcinoembryonic antigen by RT-PCR in peritoneal …”

Section: Discussionmentioning

confidence: 99%

Analysis of mRNA Quality in Freshly Prepared and Archival Papanicolaou Samples

Chuaqui

Cole²,

Cuello³

et al. 1999

Acta Cytologica

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Immunoperoxidase studies by monoclonal antibody B72.3 applied to breast aspirates: Diagnostic considerations

Cited by 11 publications

References 7 publications

Cytomorphology of Fibrocystic Change, High-Risk, and Premalignant Breast Lesions

Cytomorphology of Fibrocystic Change, High-Risk, and Premalignant Breast Lesions

Immunocytochemistry with monoclonal antibody B72.3 applied to a spectrum of benign and malignant breast aspirates — strengths and pitfalls

Analysis of mRNA Quality in Freshly Prepared and Archival Papanicolaou Samples

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