Immunophenotyping of skin-infiltrating T-cell subsets in dogs with atopic dermatitis

Sinke, Jacqueline D.; Thepen, Theo; Bihari, Ilse C.; Rutten, Victor P.M.G.; Willemse, Ton

doi:10.1016/s0165-2427(96)05763-7

Cited by 56 publications

(61 citation statements)

References 20 publications

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“…The CD4+ to CD8+ T-cell ratio is also important. A higher ratio in peripheral blood and skin with a predominance of CD4+ T-cells has been described in canine AD (Olivry et al 1997;Sinke et al 1997;Maeda et al 2004), whereas in our study, the ratio was almost identical.…”

Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

“…1997;Sinke et al 1997;Maeda et al 2004). The increase in CD4+ T-cells in dogs in association with CAD in peripheral blood has often been described (Sinke et al 1997;Maeda et al 2004), while the increase in the subpopulation of CD8+ T-cells was observed during secondary infections (Taszkun 2013). The relationship between CADESI-03 values and the absolute numbers of the given subpopulations could be connected with both stress and an inflammatory response in the subgroup of dogs with severe clinical signs of disease, whereas mainly stress seems to be the cause of their decline.…”

Section: Discussionmentioning

confidence: 99%

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Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Hoskova¹,

Svoboda²,

Satinska³

et al. 2015

Vet. Med. - Czech

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Canine atopic dermatitis (CAD) is a chronic allergic disease characterised by genetic predisposition. The exact pathogenesis of the disease is still not fully understood. Blood parameters, lymphocyte subpopulations, cytokines and other molecules involved in pro-and anti-inflammatory activities, and Th1/Th2 polarisation in peripheral blood were assessed in clinically healthy dogs and dogs with AD by haematological examination, flow cytometry and qRT-PCR. The group of diseased dogs was further divided into subgroups according to the severity of clinical signs expressed as CADESI-03 (canine atopic dermatitis extent and severity index) values. The group of dogs with CAD showed mild neutrophilia without general leucocytosis, reduced numbers of B-lymphocytes (P < 0.05), increased relative numbers of T-lymphocytes (P < 0.01) decreased absolute numbers of double-positive lymphocytes (P < 0.001) and NK cells (P < 0.05) as compared with healthy controls. The expression levels of IL-10 mRNA were significantly higher (P < 0.05) in the group with CAD. Correlation with CADESI-03 was observed in expression levels of CCL3 (P < 0.05), CCL5 (P < 0.05) and SOCS-3 mRNAs (P < 0.05). In conclusion, the disease stage investigated in the present study is characterised by a chronic inflammatory response.

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Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Hoskova¹,

Svoboda²,

Satinska³

et al. 2015

Vet. Med. - Czech

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“…In canine AD an increased presence of CD4 + and CD8 + T cells in LS skin is reported, but the ratio of CD4 + /CD8 + T cells measured in the epidermis is debated. Sinke et al (1997) observed an increased ratio of CD4 + /CD8 + T cells, whereas Olivry et al (1997) found a preferential presence of CD8 + T cells. In addition, we observed that the IFN-c expression in LS skin was increased in comparison to NLS skin.…”

Section: Discussionmentioning

confidence: 92%

“…In contrast, cell subsets and cytokine production in the skin of dogs with AD have been investigated extensively. In AD, inflammation is characterised by an influx of CD4 + and CD8 + T cells in the lesional skin (Olivry et al, 1997;Sinke et al, 1997) and a mixed cytokine profile with predominant expression of IL-6, TARC, IL-4 and IL-13 genes in the early stage followed by IFN-c, IL-12 and IL-18 later on (Olivry et al, 1999;Nuttall et al, 2002;Marsella et al, 2006). Whereas most dogs with AD have circulating allergen-specific IgE (DeBoer and Hillier, 2001), there is controversial evidence for a similar reaction phenomenon in dogs with spontaneous manifestations of CAFRs (Jackson et al, 2003;Pucheu-Haston et al, 2008;Puigdemont et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions

Veenhof

Knol

Schlotter

et al. 2011

The Veterinary Journal

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a b s t r a c tThe immunopathogenesis of cutaneous adverse food reactions (CAFRs) in dogs is unknown. Since the clinical manifestations in the skin are like those found in canine atopic dermatitis (AD), this study investigated the similarity in T cell phenotypes and gene expression of cytokines and transcription factors in CAFRs. In addition, the influence of an elimination diet on these parameters was tested.In the skin of canine CAFRs, a predominant presence of CD8 + T cells and increased expression of the IL-4, IL-13, Foxp3 and SOCS-3 genes were observed. IFN-c gene expression was increased in lesional compared to non-lesional skin. The predominance of CD8 + T cells indicates that the immunopathogenesis of CAFRs is different from that of canine AD. The elimination diet relieved clinical signs, but did not influence T cell phenotypes or expression of the cytokine and transcription factor genes in the skin of dogs with CAFRs, indicating a continuously pre-activated immune status in dogs sensitised to food constituents.

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Intradermal injection of Hsp60 induces cytokine responses in canine atopic and healthy skin

Lee

Rutten

Kooten

et al. 2008

Cell Stress and Chaperones

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The purpose of this study was to investigate the immunoregulatory potential of Hsp60 in the skin of dogs with atopic dermatitis. Three dogs with chronic atopic dermatitis and four healthy dogs were injected intradermally with Hsp60 and phosphate-buffered saline. Biopsies were taken before testing from non-injected control skin, lesional and non-lesional atopic skin, and 48 and 72 h after injection. Analysis of cytokine messenger RNA was performed using quantitative real-time polymerase chain reaction. Forty-eight hours after Hsp60 injection, a rise in interleukin (IL)-10 was found (P=0.034) with the highest expression levels in non-lesional atopic and control skin. A rise of transforming growth factor beta (P=0.015) and IL12p40 (P=0.017) was noticed 72 h after Hsp60 injection in control skin. No significant differences were observed for the expression of IL-4, IL-12p35, and interferon gamma. The results indicate that Hsp60 is able to induce cytokines of a regulatory and Th1 phenotype in the skin. Furthermore, this study seems to provide a first indication of deficient Hsp60 response in atopic dermatitis affected skin.

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Immunophenotyping of skin-infiltrating T-cell subsets in dogs with atopic dermatitis

Cited by 56 publications

References 20 publications

Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions

Intradermal injection of Hsp60 induces cytokine responses in canine atopic and healthy skin

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