Impact desolvation of electrosprayed microdroplets – a new ionization method for mass spectrometry of large biomolecules

Aksyonov, Sergei A.; Williams, Peter W.

doi:10.1002/rcm.470.abs

Cited by 25 publications

(33 citation statements)

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“…17 -20 Unfortunately, however, stable ion currents can only be obtained until the lens electrodes become contaminated by accumulated glycerol, at which time source cleaning is necessary. 21 This is the main reason for the less widespread application of MCI despite its very soft and highly sensitive desorption/ionization capabilities. The subject of this work is electrospray droplet impact (EDI) ionization technique, 22 which is a modified version of MCI.…”

Section: Aspects Of Electrospray Droplet Impact For Sims 895mentioning

confidence: 99%

Fundamental aspects of electrospray droplet impact/SIMS

2006

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A new ionization method, electrospray droplet impact ionization (EDI), has been developed for matrix-free secondary-ion mass spectrometry (SIMS). The charged droplets formed by electrospraying 1 M acetic acid aqueous solution are sampled through an orifice with a diameter of 400 microm into the first vacuum chamber, transported into a quadrupole ion guide, and accelerated by 10 kV after exiting the ion guide. The droplets impact on a dry solid sample (no matrix used) deposited on a stainless steel substrate. The secondary ions formed by the impact are transported to a second quadrupole ion guide and mass-analyzed by an orthogonal time-of-flight mass spectrometer (TOF-MS). Ten pmol of gramicidin S could be detected with the presence of as much as 10 nmol of NaCl. The ion signal for arginine disappeared with decrease in the substrate temperature below 150 K owing to the formation of ice film over the sample surface. While 10 fmol of gramicidin S could be detected for 30 min, the ionization/desorption efficiency for EDI becomes smaller with an increase in the molecular weight (MW) of a biological sample. The largest protein samples detected to date are cytochrome c and lysozyme. The high sensitivity for EDI is due to the fact that samples only a few monolayers thick are subject to desorption/ionization by EDI, with little fragmentation. A coherent phonon excitation may be the main mechanism for the desorption/ionization of the solid sample.

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Section: Aspects Of Electrospray Droplet Impact For Sims 895mentioning

confidence: 99%

Fundamental aspects of electrospray droplet impact/SIMS

2006

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“…A special vacuum flange with the nozzle situated between two windows has been built to allow observation of the electrospray source in vacuum with an optical microscope. A radiative heater consisting of a tungsten wire loop was mounted close to the nozzle to prevent ice formation [89].…”

Section: Electrospraymentioning

confidence: 99%

Droplet streams for serial crystallography of proteins

et al. 2007

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Abstract. Serial diffraction of proteins requires an injection method to deliver protein moleculespreferably uncharged, fully hydrated, spatially oriented, and with high flux -into the crossed beams of an alignment laser and a focused probe beam of electrons or X-rays of typically only a few tens of microns diameter. The aim of this work has been to examine several potential droplet sources as to their suitability for this task. We compare Rayleigh droplet sources, electrospray sources, nebulizers and aerojet-focused droplet sources using time-resolved optical images of the droplet beams. Shrinkage of droplets by evaporation as a means of removing most of the water surrounding the proteins is discussed. Experimental measurements of droplet size, conformation of proteins after passing through a Rayleigh jet, and triboelectric charging are presented and conclusions are drawn about the source configuration for serial diffraction. First experimental X-ray diffraction patterns from Rayleigh droplet beams doped with 100nm gold balls are shown.

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“…In contrast, MCI is a soft ionization method and is well suited for biopolymers. Recently, Aksyonov and Williams 69) studied the impact desolvation of electrosprayed microdroplets (IDEM) for mass spectrometry of large biomolecules. Analytes are dissolved in an electrolyte solution which is then electrosprayed in vacuum, producing highly charged micron and submicron sized droplets.…”

Section: ῃῃmentioning

confidence: 99%

Development of New Ionization Methods for GC/MS and LC/MS Interfaces

Hiraoka

2005

J. Mass Spectrom. Soc. Jpn.

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New ionization methods for GC/MS and LC/MS interface which have been developed in our laboratory will be described. The atmospheric pressure Penning ionization was developed for GC/MS interface. Its sensitivity was found to be as good as that of the electron impact ionization method. The laser spray developed for LC/MS interface, which can be regarded as the electric-field assisted MALDI, gave about one order of magnitude higher ion detection sensitivity than the conventional electrospray. In addition, laser spray can detect ions with di#erent surface activities more non-selectively than electrospray.

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Impact desolvation of electrosprayed microdroplets – a new ionization method for mass spectrometry of large biomolecules

Cited by 25 publications

References 0 publications

Fundamental aspects of electrospray droplet impact/SIMS

Fundamental aspects of electrospray droplet impact/SIMS

Droplet streams for serial crystallography of proteins

Development of New Ionization Methods for GC/MS and LC/MS Interfaces

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