Impact of EUCAST, CLSI and USCAST ceftaroline breakpoint changes on the susceptibility of methicillin-resistant Staphylococcus aureus isolates collected from US medical centres (2015–2018)

Sader, Hélio S.; Jones, Ronald N.

doi:10.1016/j.cmi.2019.12.014

Cited by 8 publications

(6 citation statements)

References 3 publications

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“…Our data confirmed discrepancies in EUCAST disk diffusion assays compared to MIC determination (both with broth microdilution or a MIC test strip) as reported before [ 19 ], and further suggested the need of a revision of disk diffusion criteria. Overall, ceftaroline resistance rates in our collection were very low and comparable to other surveillance studies on MRSA and ceftaroline [ 13 , 20 , 21 ]. This trend derived also from the recent breakpoint changes made in 2019 [ 21 , 22 ] that established higher MIC breakpoints for ceftaroline resistance interpretation.…”

Section: Resultssupporting

confidence: 86%

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Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy

et al. 2021

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Background: Ceftaroline represents a novel fifth-generation cephalosporin to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Ceftaroline susceptibility of 239 MRSA isolates was assessed by disk diffusion and a MIC test strip following both EUCAST and CLSI guidelines. Non-susceptible isolates were epidemiologically characterized by pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing, and further investigated by PCR and whole genome sequencing to detect penicillin-binding protein (PBP) mutations as well as antibiotic resistance and virulence genes. Results: Fourteen isolates out of two hundred and thirty-nine (5.8%) were non-susceptible to ceftaroline (MIC > 1 mg/L), with differences between the EUCAST and CLSI interpretations. The characterized isolates belonged to seven different pulsotypes and three different clones (ST228/CC5-t041-SCCmecI, ST22/CC22-t18014-SCCmecIV, and ST22/CC22-t022-SCCmecIV), confirming a clonal diffusion of ceftaroline non-susceptible strains. Mutations in PBPs involved PBP2a for ST228-t041-SCCmecI strains and all the other PBPs for ST22-t18014-SCCmecIV and ST22-t022-SCCmecIV clones. All isolates harbored antibiotic resistance and virulence genes with a clonal distribution. Conclusion: Our study demonstrated that ceftaroline non-susceptibile isolates belonged not only to ST228 strains (the most widespread clone in Italy) but also to ST22, confirming the increasing role of these clones in hospital infections.

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Section: Resultssupporting

confidence: 86%

“…Overall, ceftaroline resistance rates in our collection were very low and comparable to other surveillance studies on MRSA and ceftaroline [ 13 , 20 , 21 ]. This trend derived also from the recent breakpoint changes made in 2019 [ 21 , 22 ] that established higher MIC breakpoints for ceftaroline resistance interpretation.…”

Section: Resultssupporting

confidence: 86%

Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy

et al. 2021

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“…The results above also suggest the presence of a high level of resistance to clindamycin, tetracycline, and erythromycin, which are antimicrobial agents classified among the most important in veterinary medicine, especially tetracycline [67]. L. monocytogenes, from the results in Table 5, remains susceptible to the first drugs of choice in the treatment of Listeriosis as prescribed by CLSI guidelines [32]. However, limited studies have recorded high levels of L. monocytogenes resistance to clindamycin, especially from food isolates [68].…”

Section: Discussionmentioning

confidence: 87%

“…only include a few antimicrobial agents such as trimethoprim-sulfamethoxazole, ampicillin, imipenem, and penicillin; therefore, we used CLSI breakpoints for Streptococcus pneumoniae for other antimicrobial agents, and this was because the CLSI guidelines indicated that this was the organism used to interpret breakpoints for L. monocytogenes [32]. The identical 23 isolates and antibiotics had their antimicrobial susceptibility tested using the Etest (Biomerieux) with vancomycin and imipenem because there was no interpretation provided under disc diffusion in the CLSI [32]. The quality control strains included were L. monocytogenes ATCC 19118, Streptococcus pneumoniae ATCC 49619, and Staphylococcus aureus ATCC 25923.…”

Section: Phenotypic Detection Of Antimicrobial Resistance In Listeria...mentioning

confidence: 99%

“…The commonly used antibiotics are but not limited to penicillin G, ampicillin, gentamicin, erythromycin, vancomycin, chloramphenicol, ciprofloxacin, and tetracycline [31]. The Clinical and Laboratory Standards Institute (CLSI) defines the first-line antibiotics used in treating Listeriosis as penicillin G, ampicillin, Trimethoprim/sulfamethoxazole, and imipenem [32]. The use of the same drugs in the treatment of poultry is often practiced [33].…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic Resistance Patterns of Listeria Species Isolated from Broiler Abattoirs in Lusaka, Zambia

et al. 2022

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L. monocytogenes is a public health threat linked to fast foods such as broiler chickens. This study aimed to verify the occurrence of Listeria species in chickens from abattoirs and evaluate their antimicrobial resistance. In total, 150 broiler carcass swabs distributed as cloacal (n = 60), exterior surface (n = 60), and environmental (n = 30) were collected. Listeria species were characterized using biochemical tests and PCR. We conducted antibiotic resistance tests using the disc diffusion and Etest (Biomerieux, Durham, NC, USA) methods. Overall isolation of Listeria species was 15% (23/150) 95% CI (10.16–22.33), 2% (3/150) 95% CI (0.52–6.19) and 13% (20/150) 95% CI (8.53–20.08) came from environmental swabs and carcass swabs, respectively. Proportions of positive Listeria isolates were L. monocytogenes 74% (17/23), L. welshimeri 22% (5/23), and L. innocua 4% (1/23). Listeria species from the exterior carcass swabs was 61% (14/23), cloacal swabs 26% (6/23), and environmental swabs 3% (3/23). L. monocytogenes had the greatest resistance percentage to the following antibiotics: clindamycin (61%, 10/23), tetracycline 30% (7/23), and erythromycin 13%, (3/23). Isolation of L. monocytogenes in relatively high numbers, including the antimicrobial profiles, suggests a potential risk of the pathogen remaining viable in the food continuum and a public health risk to would-be consumers.

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Update on the in vitro activity of ceftaroline against Staphylococcus aureus from United States (US) medical centers stratified by infection type (2018−2020)

Sader

Castanheira

Duncan

et al. 2023

Diagnostic Microbiology and Infectious Disease

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Impact of EUCAST, CLSI and USCAST ceftaroline breakpoint changes on the susceptibility of methicillin-resistant Staphylococcus aureus isolates collected from US medical centres (2015–2018)

Cited by 8 publications

References 3 publications

Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy

Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy

Antibiotic Resistance Patterns of Listeria Species Isolated from Broiler Abattoirs in Lusaka, Zambia

Update on the in vitro activity of ceftaroline against Staphylococcus aureus from United States (US) medical centers stratified by infection type (2018−2020)

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