Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature

Oropesa‐Nuñez, Reinier; Mescola, Andrea; Vassalli, Massimo; Canale, Claudio

doi:10.3390/s21041069

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…The mechanical properties of nanometric objects have been extensively studied by force spectroscopy. AFM groups are interested in low-dimensional structures [111,112] in material science and cells [113][114][115], biological tissues [116], biological particles [117][118][119][120], and biological films [121,122] in life science. In singulo force spectroscopy (cp.…”

Section: Scientific Research Publications and Fundings Of Italian Spm...mentioning

confidence: 99%

Probing Italy: A Scanning Probe Microscopy Storyline

Dinelli

Brucale

Valle

et al. 2023

Micro

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Starting from the late 1980’s, scanning probe microscopy has progressively diffused in Italy until today. In this paper, we provide a brief account of the main historical events and a current picture of the distribution of the active groups. A survey was prepared by LimeSurvey, made of six sections asking for personal and institutional data, human resources, equipment available, fields of interest, research projects, educational/dissemination activities, and two relevant publications in the last six years. It turns out that the Italian community includes more than seventy groups and two companies. It is widely diffused, although mostly concentrated near large academic and research institutions, often in locations where prominent Italian researchers have operated. This community is active in many scientific fields and can produce research of high international quality. It shows a wide competence, as proven by the list of research works published in journals ranked within the top 20% class. The diffusion of SPM microscopes in industry is still sporadic, possibly due to extensive collaborations between the research institutions and industries themselves. The authors hope that this work might be useful to the community and beyond, and that it might stimulate the formation of a more structured network.

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Section: Scientific Research Publications and Fundings Of Italian Spm...mentioning

confidence: 99%

Probing Italy: A Scanning Probe Microscopy Storyline

Dinelli

Brucale

Valle

et al. 2023

Micro

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“…In brief, AFM observation is considered as a multipurpose technique including imaging and detection of tiny interaction forces in a few pN levels between two surfaces. The SCFS mode of AFM technique may detect the interaction between single living cells and substrate or single cell, one is on the substrate, and the other one is adhered on the cantilever with the help of cell-adhering protein coating [51].…”

Section: Measurement Of Cell-substrate Interaction Behavior By Scfsmentioning

confidence: 99%

Cell Adhesion Strength Indicates the Antithrombogenicity of Poly(2-methoxyethyl acrylate) (PMEA): Potential Candidate for Artificial Small-Diameter Blood Vessel

2022

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Poly (2-methoxyethyl acrylate) (PMEA) is a US FDA-approved biocompatible polymer, although there is insufficient work on human umbilical vein endothelial cells (HUVECs) and platelet interaction analysis on PMEA-analogous polymers. In this study, we extensively investigated HUVEC–polymer and platelet–polymer interaction behavior by measuring the adhesion strength using single-cell force spectroscopy. Furthermore, the hydration layer of the polymer interface was observed using frequency-modulation atomic force microscopy. We found that endothelial cells can attach and spread on the PMEA surface with strong adhesion strength compared to other analogous polymers. We found that the hydration layers on the PMEA-analogous polymers were closely related to their weak platelet adhesion behavior. Based on our results, it can be concluded that PMEA is a promising candidate for the construction of artificial small-diameter blood vessels owing to the presence of IW and a hydration layer on the interface.

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