Impact of feeding juvenile tench (Tinca tinca (L.)) feeds supplemented with vegetable oils on hematological indexes and liver histology

Abstract

Citation: Demska-Zakêoe K., Zakêoe Z., Ziomek E., Jarmo³owicz S. 2012 -Impact of feeding juvenile tench (Tinca tinca (L.)) feeds supplemented with vegetable oils on hematological indexes and liver histology -Arch. Abstract. The aim of the study was to identify the impact of feeding juvenile tench commercial feed supplemented with fish oil, linseed oil, arachide oil, and rapeseed oil on the hematology, cytology, and histology indexes of the liver. The fish were reared in a recirculating system and fed feed containing 470 g protein kg -1 feed and 120 g fat kg -1 feed (1000 g of base feed containing 70 g fat kg -1 feed was supplemented with 50 g of additional fat, i.e., fish oil (FO) or vegetable oilslinseed oil (LO), arachide oil (AO), or rapeseed oil (RO)). Statistically significant inter-group differences were noted for hematocrit (Ht), hemoglobin (Hb), red blood cell (RBC) counts, and other red blood cell indexes, including mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) (P < 0.05). The lowest Ht and Hb values were noted in fish fed feed supplemented with fish oil (group FO), while the highest was noted in the tench from group AO. These differences were mainly caused by the occurrence of smaller erythrocytes in the peripheral blood of the fish from the FO group. No pathological changes were observed in the blood cells, which was in contrast to the parenchymal cells of the liver. Congestion, lipid vacuolization in hepatocytes, regressive changes in cytoplasm density, nuclear chromatin, and nucleus disintegration were observed in all fish. These changes were more pronounced in groups LO and AO. The fish from these groups were characterized by smaller hepatocytes and significantly higher nucleocytoplasmic ratios (P < 0.05).

Erythrocytes in teleost fishes: a review

“…According to Babalola et al (2009), erythrocyte count in Heterobranchus longifilis was significantly affected by a dietary lipid source (higher in fish fed with sunflower and poultry lipids compared to those fed with other vegetable or animal fat). A significant effect of the lipid source on erythrocyte count was confirmed by Demska-Zakęś et al (2012) for Tinca tinca. Rigos et al (2010) reported an increase in RBC of S. aurata fed with food supplemented with iron.…”

Effect of feeding various levels of poultry by-product meal on the blood parameters, filet composition and structure of female tenches (Tinca tinca)

“…It is likely that a histological assessment of the liver with such altered external structure would reveal pathological changes (e.g. hepatocyte congestion, hepatocyte lipid vacuolization) that were described in experiments in which tenches received feed containing 50 g/kg arachide or linseed oil (Demska‐Zakęś, Zakęś, Ziomek & Jarmołowicz, ). Interesting are also the results of histological analyses in the tilapia published by Aydin, Gümüş & Balci ().…”

Effect of different feed ingredients on digestive enzymes activity and on the histology of liver and intestine in Labeo rohita Hamilton, 1822

“…2). Demska-Zakes et al (2012) reported congestion, vacuolisation in hepatocytes, nuclear chromatin and nucleus disintegration when supplementary feed containing vegetable oil was offered to juvenile tench (Tinca tinca). Vacuolation in hepatocytes with pyknotic nuclei were observed in fish fed on FM diet Effect of different feed ingredients on digestive enzymes activity in Labeo rohita (Fig.…”