Impact of fluorescent dyes on the physicochemical parameters of microbubbles stabilized by albumin-dye complex

Barmin, Roman A.; Rudakovskaya, Polina G.; Chernyshev, Vasiliy S.; Guslyakova, Olga I.; Sindeeva, Olga A.; Prikhozhdenko, Ekaterina S.; Bratashov, Daniil N.; Abdurashitov, Arkady S.; Maksimova, Elizaveta A.; Demina, Polina A.; Khaydukov, E. V.; Gayer, Alexey V.; Shirshin, Evgeny A.; Solovev, Alexander A.; Mei, Yongfeng; Gorin, Dmitry A.

doi:10.1016/j.colsurfa.2022.129095

Cited by 11 publications

(11 citation statements)

References 69 publications

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“…The expedient choice of characterization method is essential to fully evaluate the properties of protein MBs, as recently demonstrated for dye-labeled BSA MBs [138]. In this section, we summarize existing approaches in MB characterization, providing examples in Figure 5.…”

Section: Advanced Characterization Of Mbs With a Protein Shellmentioning

confidence: 99%

“…Few measurement approaches could be considered. The traditional one is based on combining optical microscopy (OM) with a cell counter, where MBs are counted manually in a time-consuming manner [19,21,90,138,141,142]. Another approach involves Coulter counter (CC) measurements, based on resistive pulse sensing for counting and sizing particles suspended in electrolytes.…”

Section: Advanced Characterization Of Mbs With a Protein Shellmentioning

confidence: 99%

“…The introduction of fluorescent small molecules can be confirmed by confocal microscopy (Figure 5f) [84,109,133,143,147], while STED or fluorescent lifetime imaging microscopy (FLIM) may offer higher image resolution combined with insight into the homogeneous/heterogeneous distribution of fluorescent components in the shell and distribution of dye among MB population (Figure 5g) [148]. Unfortunately, FLIM and STED methods are barely described for protein MBs [138]. The presence of inorganic nanoparticles can be confirmed with TEM (Figure 5h,i) [89,145].…”

Section: Advanced Characterization Of Mbs With a Protein Shellmentioning

confidence: 99%

“…The storage stability of protein MBs can be evaluated by zeta-potential measurements, in addition to methods described for size/concentration characterization [19,70,109,138,149]. However, significant attention should be given to the stability of MBs in blood-mimicking solutions, resulting in the evaluation of a parameter similar to in vivo circulation time.…”

Section: Advanced Characterization Of Mbs With a Protein Shellmentioning

confidence: 99%

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Microbubbles Stabilized by Protein Shell: From Pioneering Ultrasound Contrast Agents to Advanced Theranostic Systems

et al. 2022

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Ultrasound is a widely-used imaging modality in clinics as a low-cost, non-invasive, non-radiative procedure allowing therapists faster decision-making. Microbubbles have been used as ultrasound contrast agents for decades, while recent attention has been attracted to consider them as stimuli-responsive drug delivery systems. Pioneering microbubbles were Albunex with a protein shell composed of human serum albumin, which entered clinical practice in 1993. However, current research expanded the set of proteins for a microbubble shell beyond albumin and applications of protein microbubbles beyond ultrasound imaging. Hence, this review summarizes all-known protein microbubbles over decades with a critical evaluation of formulations and applications to optimize the safety (low toxicity and high biocompatibility) as well as imaging efficiency. We provide a comprehensive overview of (1) proteins involved in microbubble formulation, (2) peculiarities of preparation of protein stabilized microbubbles with consideration of large-scale production, (3) key chemical factors of stabilization and functionalization of protein-shelled microbubbles, and (4) biomedical applications beyond ultrasound imaging (multimodal imaging, drug/gene delivery with attention to anticancer treatment, antibacterial activity, biosensing). Presented critical evaluation of the current state-of-the-art for protein microbubbles should focus the field on relevant strategies in microbubble formulation and application for short-term clinical translation. Thus, a protein bubble-based platform is very perspective for theranostic application in clinics.

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Section: Advanced Characterization Of Mbs With a Protein Shellmentioning

confidence: 99%

Section: Advanced Characterization Of Mbs With a Protein Shellmentioning

confidence: 99%

Section: Advanced Characterization Of Mbs With a Protein Shellmentioning

confidence: 99%

Section: Advanced Characterization Of Mbs With a Protein Shellmentioning

confidence: 99%

See 2 more Smart Citations

Microbubbles Stabilized by Protein Shell: From Pioneering Ultrasound Contrast Agents to Advanced Theranostic Systems

et al. 2022

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“…In recent years, MB are also increasingly explored as drug delivery vehicles based on their drug loading capacities, US stimuli-responsiveness, and established shell functionalization routes. − The shell of MB is mainly prepared with low-molecular-weight surfactants, lipids, proteins, or polymers. − For contrast-enhanced US imaging, MB with thinner shells ( e.g. , lipid-based MB) tend to be preferred since they usually display superior acoustic responses (in terms of echogenicity and oscillation profile), while for drug delivery applications, MB with thicker shells ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Engineering the Acoustic Response and Drug Loading Capacity of PBCA-Based Polymeric Microbubbles with Surfactants

et al. 2022

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Gas-filled microbubbles (MB) are routinely used in the clinic as ultrasound contrast agents. MB are also increasingly explored as drug delivery vehicles based on their ultrasound stimuli-responsiveness and well-established shell functionalization routes. Broadening the range of MB properties can enhance their performance in both imaging and drug delivery applications. This can be promoted by systematically varying the reagents used in the synthesis of MB, which in the case of polymeric MB include surfactants. We therefore set out to study the effect of key surfactant characteristics, such as the chemical structure, molecular weight, and hydrophilic–lipophilic balance on the formation of poly(butyl cyanoacrylate) (PBCA) MB, as well as on their properties, including shell thickness, drug loading capacity, ultrasound contrast, and acoustic stability. Two different surfactant families (i.e., Triton X and Tween) were employed, which show opposite molecular weight vs hydrophilic–lipophilic balance trends. For both surfactant types, we found that the shell thickness of PBCA MB increased with higher-molecular-weight surfactants and that the resulting MB with thicker shells showed higher drug loading capacities and acoustic stability. Furthermore, the higher proportion of smaller polymer chains of the Triton X-based MB (as compared to those of the Tween-based ones) resulted in lower polymer entanglement, improving drug loading capacity and ultrasound contrast response. These findings open up new avenues to fine-tune the shell properties of polymer-based MB for enhanced ultrasound imaging and drug delivery applications.

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Magnetically navigated microbubbles coated with albumin/polyarginine and superparamagnetic iron oxide nanoparticles

Gusliakova,

Kurochkin,

Barmin

et al. 2024

Biomaterials Advances

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Impact of fluorescent dyes on the physicochemical parameters of microbubbles stabilized by albumin-dye complex

Cited by 11 publications

References 69 publications

Microbubbles Stabilized by Protein Shell: From Pioneering Ultrasound Contrast Agents to Advanced Theranostic Systems

Microbubbles Stabilized by Protein Shell: From Pioneering Ultrasound Contrast Agents to Advanced Theranostic Systems

Engineering the Acoustic Response and Drug Loading Capacity of PBCA-Based Polymeric Microbubbles with Surfactants

Magnetically navigated microbubbles coated with albumin/polyarginine and superparamagnetic iron oxide nanoparticles

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