Impact of human adipose tissue-derived stem cells on dermatofibrosarcoma protuberans cells in an indirect co-culture: an in vitro study

Abstract

Background Autologous adipose tissue transfer may be performed for aesthetic needs following the resection of dermatofibrosarcoma protuberans (DFSP), the most common cutaneous soft tissue sarcoma, excluding Kaposi sarcoma. The regenerative effectiveness of cell-assisted lipotransfer is dependent on the presence of adipose tissue-derived stem cells (ADSCs). This is the first study to evaluate the potential oncological risks as ADSCs could unintentionally be sited within the proximity of the tumor microenvironment of DFSP cells. Methods Primary DFSP cells were indirectly co-cultured with ADSCs in a conditioned medium or in a Transwell system. The impact was analyzed by assessing proliferation, migration, invasion, angiogenesis, and tumor-associated genes and proteins. Results of these assays were compared between co-culture and mono-culture conditions. Results Our experimental results showed that ADSCs were able to promote proliferation, migration, invasion, and angiogenesis of DFSP cells; this was accompanied by a significant increase in the expression levels of beta-type platelet-derived growth factor receptor, collagen type I alpha 1 chain, vascular endothelial growth factor, hepatocyte growth factor, and basic fibroblast growth factor. Conclusions The current report clearly demonstrates that ADSCs can enhance different malignant properties of DFSP cells in vitro, which should not be neglected when considering the clinical use of human ADSCs and its related derivatives in skin regenerative therapies.

Carbon Ions Suppress Angiogenesis and Lung Metastases in Melanoma by Targeting CXCL10

“…Corning co-culture inserts (0.4-µm pores; 6 wells) were used for co-culturing experiments based on a previous report ( 31 ). B16F10 cells (2 3 10 5 cells per well) were immediately collected and plated in the upper chambers of a co-culture system after the completion of the transfection and irradiation.…”

“…The CCK-8(YEASEN) technique was employed to assess cell proliferation and performed as described previously ( 31 ). After 48 h of co-culture, HUVECs (3 3 10 4 /well) were seeded in 96-well plates.…”

“…The 24-well transwell chambers were utilized to conduct migration tests (8-mm pore size; Corning) in vitro ( 31 ). B16F10 cells (5 3 10 4 cells per well) and HUVECs (3 3 10 4 cells per well) from the co-culture were distributed into the bottom and upper unit, respectively.…”

“…The tube formation assay was conducted as previously described ( 31 ). HUVECs (3 3 10 5 ) harvested from the co-culture system were added into 50 µl DMEM with 10% fetal bovine serum and grown in a 96-well plate coated with 50 µl matrigel (BD Biosciences).…”

3D Tumor Models in Urology

“…The advantages of these cultures are their availability and easy handling. For instance, transwell cultures allow analyses of secreted factors from one cell type and their effects on a spatially separated second cell type [ 4 , 5 ].…”