Impact of indigenous microorganisms on Escherichia coli O157:H7 growth in cured compost

Kim, Jin‐Kyung; Miller, Cortney M.; Shepherd, Marion W.; Liu, Xiaohua; Jiang, Xiuping

doi:10.1016/j.biortech.2011.07.055

Cited by 11 publications

(8 citation statements)

References 36 publications

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“…As noted by Kim et al [18] and exemplified in this research, steam sterilization can be used to alter microbiota populations in compost and soil. Kim et al [18] autoclaved compost for various lengths of time and temperatures to obtain approximate concentrations of indigenous mesophilic microorganisms within compost for subsequent analysis of E. coli O157 survival.…”

Section: Plos Onementioning

confidence: 79%

“…It has been suggested that soils with a more diverse bacterial taxa profile are less likely to be colonized by a foreign microbe introduced into the soil [15]. This concept has been evaluated with a focus on foodborne pathogen establishment and survival in soils based on biodiversity [10,[16][17][18][19][20][21][22][23] although few studies have identified specific bacterial taxa within soils that may cause variation in pathogen survival [19]. Previous studies have investigated the influence of biotic factors on pathogen survival by altering and/or eliminating microbial populations in soil [17,23] or compost [18] via steam sterilization (autoclaving) for comparison with natural (unautoclaved) soils.…”

Section: Introductionmentioning

confidence: 99%

“…This concept has been evaluated with a focus on foodborne pathogen establishment and survival in soils based on biodiversity [10,[16][17][18][19][20][21][22][23] although few studies have identified specific bacterial taxa within soils that may cause variation in pathogen survival [19]. Previous studies have investigated the influence of biotic factors on pathogen survival by altering and/or eliminating microbial populations in soil [17,23] or compost [18] via steam sterilization (autoclaving) for comparison with natural (unautoclaved) soils. Greater survival of E. coli O157 has been observed in autoclaved soils when compared to natural soils [17,20,23,24], which suggests that certain soil microorganisms that are reduced by heat treatments likely contribute to the reduction of E. coli O157 in soils.…”

Section: Introductionmentioning

confidence: 99%

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Survival of Escherichia coli O157 in autoclaved and natural sandy soil mesocosms

Baker

Lee

et al. 2020

PLoS ONE

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While the soil microbiome may influence pathogen survival, determining the major contributors that reduce pathogen survival is inconclusive. This research was performed to determine the survival of E. coli O157 in autoclaved and natural (unautoclaved) sandy soils. Soils were inoculated with three different E. coli O157 strains (stx1+/stx2+, stx1-/stx2-, and stx1-/ stx2+), and enumerated until extinction at 30˚C. There was a significant difference in the survival of E. coli O157 based on soil treatment (autoclaved versus natural) at 30˚C on days 1 (P = 0.00022), 3, (P = 2.53e-14), 7 (P = 5.59e-16), 14 (P = 1.072e-12), 30 (P = 7.18e-9), and 56 (P = 0.00029), with greater survival in autoclaved soils. The time to extinction (two consecutive negative enrichments) for all three strains was 169 and 84 days for autoclaved and natural soils, respectively. A separate E. coli O157 trial supplemented with 16S rRNA gene sequencing of the soil microbiome was performed at 15˚C and 30˚C on days 0, 7, 14, and 28 for each soil treatment. Greater species richness (Chao1, P = 2.2e-16) and diversity (Shannon, P = 2.2e-16) was observed in natural soils in comparison with autoclaved soils. Weighted UniFrac (beta-diversity) showed a clear distinction between soil treatments (P = 0.001). The greatest reduction of E. coli O157 was observed in natural soils at 30˚C, and several bacterial taxa positively correlated (relative abundance) with time (day 0 to 28) in these soils (P < 0.05), suggesting that the presence of those bacteria might cause the reduction of E. coli O157. Taken together, a clear distinction in E. coli O157 survival, was observed between autoclaved and natural soils along with corresponding differences in microbial diversity in soil treatments. This research provides further insights into the bacterial taxa that may influence E. coli O157 in soils.

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Section: Plos Onementioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Survival of Escherichia coli O157 in autoclaved and natural sandy soil mesocosms

Baker

Lee

et al. 2020

PLoS ONE

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“…The sharp decrease of the order Pseudomonadales could be explained by the effects of high temperature on Gram-negative bacteria (Dees and Ghiorse, 2001 ). On the other hand, as previously reported, the type of indigenous microorganisms such as actinomycetes and fungi is critical for the suppression of pathogens growth in compost (Kim et al ., 2011 ). This might be another reason why the order Pseudomonadales decreased.…”

Section: Resultsmentioning

confidence: 99%

Metaproteomics reveals major microbial players and their biodegradation functions in a large‐scale aerobic composting plant

Liu

et al. 2015

Microbial Biotechnology

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Composting is an appropriate management alternative for municipal solid waste; however, our knowledge about the microbial regulation of this process is still scare. We employed metaproteomics to elucidate the main biodegradation pathways in municipal solid waste composting system across the main phases in a large-scale composting plant. The investigation of microbial succession revealed that Bacillales, Actinobacteria and Saccharomyces increased significantly with respect to abundance in composting process. The key microbiologic population for cellulose degradation in different composting stages was different. Fungi were found to be the main producers of cellulase in earlier phase. However, the cellulolytic fungal communities were gradually replaced by a purely bacterial one in active phase, which did not support the concept that the thermophilic fungi are active through the thermophilic phase. The effective decomposition of cellulose required the synergy between bacteria and fungi in the curing phase.

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“…Primers and methods used for PCR amplification, purification of the PCR products, and sequencing were performed according to the method described by Kim et al (2011). The amplified PCR products were sequenced by the Clemson University Genomic Institute.…”

Section: Sequencing Analysismentioning

confidence: 99%

Rapid identification of Campylobacter jejuni from poultry carcasses and slaughtering environment samples by real-time PCR

et al. 2014

Self Cite

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The objective of this study was to develop a real-time PCR assay for rapid identification of Campylobacter jejuni and to apply the method in analyzing samples from poultry processing. A C. jejuni-specific primer set targeting a portion of the C. jejuni hippuricase gene was developed. The specificity of the newly designed primer pair was verified using 5 C. jejuni strains and 20 other bacterial strains. Sensitivity was determined to be as low as 1 genome copy per reaction. A total of 73 samples were collected at different sites along the processing line during 2 visits to a poultry slaughterhouse and were examined by direct plating onto modified charcoal cefoperazone deoxycholate agar or after enrichment in Bolton broth followed by plating on modified charcoal cefoperazone deoxycholate agar. The newly developed real-time PCR assay was used to identify the presumptive colonies as belonging to C. jejuni. A real-time PCR assay targeting 16S ribosomal RNA was also applied to determine Campylobacter spp. prevalence. Results from the real-time PCR analysis indicated considerable variability in Campylobacter contamination, with incidence rates of 72.7 and 27.6% for sampling days A and B, respectively. Campylobacter was isolated from 100% of prescalded and preeviscerated carcasses on sampling day A. In contrast, on sampling day B, the highest number of Campylobacter-positive carcasses was recovered after evisceration (60%). The chilling process significantly reduced (P < 0.05) Campylobacter population, but the percentage of positive samples on sampling day A increased to 80%. All samples collected from the processing environment, except scalding tank 3 and the prechiller and chiller tanks, were 100% positive on day A, whereas no campylobacters were isolated from machinery on sampling day B. Our results revealed the widespread of C. jejuni in poultry processing and proved that the newly developed real-time PCR assay is a simple, specific, and inexpensive method for rapid C. jejuni identification. The newly developed PCR method can be easily used in laboratories for reliable and unambiguous identification of C. jejuni in poultry samples.

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Impact of indigenous microorganisms on Escherichia coli O157:H7 growth in cured compost

Cited by 11 publications

References 36 publications

Survival of Escherichia coli O157 in autoclaved and natural sandy soil mesocosms

Survival of Escherichia coli O157 in autoclaved and natural sandy soil mesocosms

Metaproteomics reveals major microbial players and their biodegradation functions in a large‐scale aerobic composting plant

Rapid identification of Campylobacter jejuni from poultry carcasses and slaughtering environment samples by real-time PCR

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