Impact of oral meloxicam on circulating physiological biomarkers of stress and inflammation in beef steers after long-distance transportation1

Engen, N. K. Van; Stock, Michelle L.; Engelken, Terry J.; Vann, R. C.; Wulf, Larry W.; Karriker, Locke A.; Busby, W. Darrell; Lakritz, Jeffrey; Carpenter, A.J.; Bradford, B.J.; Hsu, Walter H.; Wang, Chong; Coetzee, Johann F.

doi:10.2527/jas.2013-6857

Cited by 68 publications

(51 citation statements)

References 46 publications

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“…Stress induced induction of serum Hp still involves release of production of cytokines and hepatic expression of Hp and other APP. [53] A recent study demonstrated the appearance of Hp-MMP 9 in serum after transportation suggesting transportation stress is also associated with measurable responses of neutrophils [52,54]. These studies support the further evaluation of Hp-MMP 9 complex as a marker of early inflammation.…”

Section: Discussionmentioning

confidence: 61%

Characterization of the contributions of Hp-MMP 9 to the serum acute phase protein response of lipopolysaccharide challenged calves

et al. 2014

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BackgroundBovine respiratory disease (BRD) is a costly feature of modern cattle production. Early and accurate detection of BRD may prove useful in the successful management of this disease. The primary objective of the study was to define the time course of covalent complexes of neutrophil, haptoglobin (Hp) and matrix metalloproteinase 9 (Hp-MMP 9) in serum after intravenous lipopolysaccharide (LPS) in comparison to traditional markers. Our hypothesis was that serum concentrations of neutrophil Hp-MMP 9 provides information distinct from traditional acute phase protein markers. To characterize the neutrophil responses to lipopolysaccharide (E. coli; O111:B4; 2.5 μg/kg body weight), nine healthy, Jersey calves (65-82 days of age; 74.5 ± 13.1 kg) were challenged and physiologic parameters, peripheral blood cell counts and serum cortisol (C), Hp-MMP 9, Hp, alpha1-acid glycoprotein (AGP), serum amyloid A (SAA) were obtained starting 24 hours before to 96 hours post-LPS challenge.ResultsPhysiologic parameters (temperature, pulse, respiratory rate) and attitude assessed at each time point indicated that LPS challenge resulted in rapid onset of depression, tachypnea, leukopenia, neutropenia and lymphopenia within 1 hour. Serum C concentrations were significantly increased by 1 hour post-LPS. Serum Hp-MMP 9 complexes were detectable in serum by 0.5 hours and peaked at 16 h, serum total Hp remained <10 μg/mL until 8 hours post LPS infusion and were significantly greater than baseline by 12 hours post-LPS infusion. Serum amyloid A concentrations increased significantly by 8 hours post LPS. Serum concentrations of AGP increased significantly by 16 hours post LPS. Serum concentrations of Hp, SAA and AGP remained significantly greater than baseline out to 96 hours post-LPS. The total systemic exposure to traditional makers is significantly greater than from Hp-MMP 9ConclusionUsing a well described model for acute phase protein responses, the data demonstrate that serum neutrophil Hp-MMP 9 complexes appear sooner and decline more rapidly than other acute phase proteins (APP). Since Hp-MMP9 is stored pre-formed, it provides information specifically addressing the LPS-induced activation of bovine neutrophils. Contributions of Hp-MMP 9 to the serum acute phase protein response may provide useful information, independent of hepatic responses, in diagnosis of acute inflammation.

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Section: Discussionmentioning

confidence: 61%

Characterization of the contributions of Hp-MMP 9 to the serum acute phase protein response of lipopolysaccharide challenged calves

et al. 2014

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“…Substance P (SP) concentrations were analyzed as described by Van Engen et al (2014) using nonextracted plasma collected in EDTA at 0. 5, 0.75, 1, 2, 4, 6, 8, 10, 12, 24, 48, 72, and 96 h (-10 min) after dehorning.…”

Section: Substance Pmentioning

confidence: 99%

Impact of carprofen administration on stress and nociception responses of calves to cautery dehorning1

Stock

Barth

Engen

et al. 2016

Journal of Animal Science

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“…Substance P (SP) concentrations were analyzed as described by Van Engen et al (2014) using nonextracted plasma. Samples were assayed in duplicate with the reported concentration equaling the average SP concentration between duplicates.…”

Section: Substance Pmentioning

confidence: 99%

“…Dehorning or disbudding cattle is a management procedure commonly performed on nearly 95% of US dairy operations (USDA, 2010). Horns or horn buds are primarily removed to accommodate production practices and prevent economic loss due to carcass bruising.…”

Section: Introductionmentioning

confidence: 99%

“…Horns or horn buds are primarily removed to accommodate production practices and prevent economic loss due to carcass bruising. Many techniques have been described, with the use of a hot iron reported to be the most commonly employed method in the United States (USDA, 2010). Although the AVMA (2012) supports methods to minimize pain and distress associated with disbudding and dehorning, methods to evaluate this response can be challenging given the need to use indirect measures to assess an affect state.…”

Section: Introductionmentioning

confidence: 99%

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The effects of firocoxib on cautery disbudding pain and stress responses in preweaned dairy calves

Stock

Millman

Barth

et al. 2015

Journal of Dairy Science

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Perioperative analgesic effects of oral firocoxib following cautery disbudding were investigated in preweaned calves. Twenty Holstein calves approximately 4 to 6wk old received a single oral dose of firocoxib, a nonsteroidal antiinflammatory, at 0.5mg/kg (n=10) or placebo (n=10) in a randomized controlled clinical trial. Responses, including ocular temperature determined by infrared thermography, pressure algometry measuring mechanical nociception threshold, and heart rate, were evaluated at 2, 4, 7, 8, and 24h after cornual nerve block and cautery disbudding. Blood samples were collected over 96h and analyzed for plasma cortisol and substance P concentrations by RIA. Additionally, ex vivo prostaglandin E2 concentrations were determined over a 72-h study period using an enzyme immunoassay. Data were analyzed using a linear mixed effects model with repeated measures. An inhibition of ex vivo prostaglandin E2 synthesis was observed from 12 to 48h following disbudding in calves treated with firocoxib. Cautery disbudding was associated with an increased nociception for the duration of sampling (24h). During the initial 24-h period following disbudding, no difference in response between treatment groups was noted. Following 24h, mean cortisol concentrations diverged between the 2 study groups with placebo-treated calves having increased cortisol concentrations at approximately 48h after disbudding. Furthermore, the overall integrated cortisol response as calculated as area under the effect curve tended to be reduced in firocoxib-treated calves. The prolonged effects of cautery dehorning require further investigation. Moreover, the effect of firocoxib on cortisol reduction observed in this study requires additional exploration.

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Impact of oral meloxicam on circulating physiological biomarkers of stress and inflammation in beef steers after long-distance transportation1

Cited by 68 publications

References 46 publications

Characterization of the contributions of Hp-MMP 9 to the serum acute phase protein response of lipopolysaccharide challenged calves

Characterization of the contributions of Hp-MMP 9 to the serum acute phase protein response of lipopolysaccharide challenged calves

Impact of carprofen administration on stress and nociception responses of calves to cautery dehorning1

The effects of firocoxib on cautery disbudding pain and stress responses in preweaned dairy calves

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