Introduction:
Curcumin is a naturally occurring compound that has antioxidant properties,
acts as a hepatoprotective, and lowers lipid peroxidation. However, curcumin's low solubility and bioavailability are its primary drawbacks and prevent its use as a therapeutic agent. In this study, curcumin
nanoparticles will be created using the ultrasonic-assisted extraction method, and their effectiveness
against paracetamol-induced changes in ALT, AST, SOD, MDA, and TNF-α will be compared to that
of pure curcumin.
Purpose:
This study aimed to determine the hepatoprotective effect of curcumin nanoparticles in paracetamol-induced rats as a model for liver injury
Methods:
Thirty-six male Wistar rats, aged 6 to 8 weeks, with a minimum weight of 120 grams, were
used in an experimental laboratory investigation with a post-test-only group design. Rats in each group
received 100 mg/kgBW pure curcumin, 100 mg/kgBW curcumin nanoparticles, and 50 mg/kgBW curcumin nanoparticles for 7 days before paracetamol induction. On day 8, 300 mg/kgBW of paracetamol
was intraperitoneally injected to cause liver damage. One of the groups received NAC as an antidote 10
hours after paracetamol induction. Detection of ALT and AST using a Chemistry Analyzer. ELISA approach for the detection of SOD, MDA, and TNF-α. The Roenigk score was calculated by two examiners after the liver histopathology preparations were stained using the Hematoxylin-Eosin method. Post
hoc analyses were performed after the One Way Annova and Kruskal Wallis tests to examine the data.
Results:
According to PSA results, the smallest formula that formed curcumin nanoparticles (10.2 nm)
was 8 g of curcumin formula mixed with a mixture of Tween 20 4.5 ml, Kolliphor EL 1.5 ml, Propylene
Glycol 1.5 ml, and Capryol 90 1 ml for 21 minutes using an ultrasonic process. MDA and TNF-α levels,
as well as the liver's histological Roenigk score, were significantly lower in the 100 mg/kgBB pure curcumin group (C100) when compared to the model group (model). The levels of AST, MDA, TNF-α,
and the liver histopathology score were significantly lower in the 100 mg/kgBB (NC100) and 50
mg/kgBB (NC50) curcumin nanoparticle groups compared to the model group (model) and pure curcumin group (C100) (p< 0.05).
