Impacts of calcium signal transduction on the fermentation production of antitumor ganoderic acids by medicinal mushroom Ganoderma lucidum

Xu, Yining; Zhong, Jian‐Jiang

doi:10.1016/j.biotechadv.2011.10.001

Cited by 80 publications

(69 citation statements)

References 45 publications

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“…All of these results are consistent with the idea that Nox-generated ROS positively regulates GA biosynthesis via the Ca 2+ -signaling pathway (Mu et al, 2014). Additionally, the promoters of the GA biosynthetic key enzyme genes (hmgr, osc and sqs) have also been reported to contain a putative Crz (calcineurin-responsive zinc finger transcription factor)-binding element suggesting a potential role for the Ca 2+ signaling pathway in GA biosynthesis (Xu and Zhong, 2012). In the present study, the expression of Crz was associated with ROS accumulation.…”

Section: Gpx-regulated Ros Regulated Ga Biosynthesis Via the Ca 2+ Sisupporting

confidence: 81%

Functional analysis of the role of glutathione peroxidase (GPx) in the ROS signaling pathway, hyphal branching and the regulation of ganoderic acid biosynthesis in Ganoderma lucidum

Shi

Chen

et al. 2015

Fungal Genetics and Biology

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Section: Gpx-regulated Ros Regulated Ga Biosynthesis Via the Ca 2+ Sisupporting

confidence: 81%

Functional analysis of the role of glutathione peroxidase (GPx) in the ROS signaling pathway, hyphal branching and the regulation of ganoderic acid biosynthesis in Ganoderma lucidum

Shi

Chen

et al. 2015

Fungal Genetics and Biology

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“…Extracting Ganoderma spores oil from broken spores by supercritical CO 2 extraction technology has been developed in recent years, and several studies have since examined the antitumor effect of Ganoderma spores oil (17,18). In the present study, a G1 arrest was detected in K562 cells treated with Ganoderma spores oil.…”

Section: Discussionmentioning

confidence: 47%

Antitumor effects and mechanisms of Ganoderma extracts and spores oil

Chen

Li³

et al. 2016

Oncology Letters

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Abstract. Ganoderma lucidum is a popular herbal medicine used in China to promote health. Modern studies have disclosed that the active ingredients of Ganoderma can exhibit several effects, including antitumor effects and immunomodulation. The present study evaluated the antitumor effects of self-prepared Ganoderma extracts and spores oil, and investigated the possible underlying mechanisms by observing the effects of the extracts and oil on topoisomerases and the cell cycle. The results showed that Ganoderma extracts and spores oil presented dose-dependent inhibitory effects on tumor cells. The half maximal inhibitory concentration (IC 50 ) values of Ganoderma extracts on HL60, K562 and SGC-7901 cells for 24 h were 0.44, 0.39 and 0.90 mg/ml, respectively; for Ganoderma spores oil, the IC 50 values were 1.13, 2.27 and 6.29 mg/ml, respectively. In the in vivo study, the inhibitory rates of Ganoderma extracts (4 g/kg/d, intragastrically) on S180 and H22 cells were 39.1 and 44.6%, respectively, and for Ganoderma spores oil (1.2 g/kg/d, intragastrically) the inhibitory rates were 30.9 and 44.9%, respectively. Ganoderma extracts and spores oil inhibited the activities of topoisomerase I and II. Ganoderma spores oil was shown block the cell cycle at the transition between the G1 and S phases and induce a marked decrease in cyclin D1 levels in K562 cells, with no significant change in cyclin E level. These results suggest that the Ganoderma extracts and spores oil possessed antitumor effects in the in vitro and in vivo studies. The antitumor mechanisms of the extracts and spores oil were associated with inhibitory effects on topoisomerase I and II activities, and for Ganoderma spores oil, the antitumor effects may also be associated with decreased cyclin D1 levels, thus inducing G1 arrest in the cell cycle.

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“…Previous work by Zhong et al . and our lab have demonstrated that ROS and Ca 2+ are able to regulate GA biosynthesis 18, 24, 25 . Sodium and manganese have been shown to trigger Ca 2+ signaling, which, in turn, leads to an enhancement of GA production 26, 27 .…”

Section: Discussionmentioning

confidence: 78%

Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum

You

Tien

Lee

et al. 2017

Sci Rep

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Apoptosis is an essential physiological process that controls many important biological functions. However, apoptosis signaling in relation to secondary metabolite biosynthesis in plants and fungi remains a mystery. The fungus Ganoderma lucidum is a popular herbal medicine worldwide, but the biosynthetic regulation of its active ingredients (ganoderic acids, GAs) is poorly understood. We investigated the role of 3′,5′-cyclic adenosine monophosphate (cAMP) signaling in fungal apoptosis and GA biosynthesis in G. lucidum. Two phosphodiesterase inhibitors (caffeine and 3-isobutyl-1-methylxanthine, IBMX) and an adenylate cyclase activator (sodium fluoride, NaF) were used to increase intracellular cAMP levels. Fungal apoptosis was identified by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay and a condensed nuclear morphology. Our results showed that GA production and fungal apoptosis were induced when the mycelium was treated with NaF, caffeine, or cAMP/IBMX. Downregulation of squalene synthase and lanosterol synthase gene expression by cAMP was detected in the presence of these chemicals, which indicates that these two genes are not critical for GA induction. Transcriptome analysis indicated that mitochondria might play an important role in cAMP-induced apoptosis and GA biosynthesis. To the best of our knowledge, this is the first report to reveal that cAMP signaling induces apoptosis and secondary metabolite production in fungi.

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Impacts of calcium signal transduction on the fermentation production of antitumor ganoderic acids by medicinal mushroom Ganoderma lucidum

Cited by 80 publications

References 45 publications

Functional analysis of the role of glutathione peroxidase (GPx) in the ROS signaling pathway, hyphal branching and the regulation of ganoderic acid biosynthesis in Ganoderma lucidum

Functional analysis of the role of glutathione peroxidase (GPx) in the ROS signaling pathway, hyphal branching and the regulation of ganoderic acid biosynthesis in Ganoderma lucidum

Antitumor effects and mechanisms of Ganoderma extracts and spores oil

Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum

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