Impaired chemotactic responses of bronchoalveolar leukocytes in experimental pneumocniosis

Donaldson, Kenneth; Brown, Gillian; Brown, David M.; Slight, Joan; Robertson, Marcus; Davis, J. Michael

doi:10.1002/path.1711600113

Cited by 27 publications

(17 citation statements)

References 18 publications

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“…TiQ dust is commonly used as a negative control dust in studies of pulmonary fibrosis [9,10,23]. Consistent with the hypothesis that NAGase concentration in lavage fluid reflects the fibrogenic potential of instilled dusts, generic anthracite and bituminous dust exposures produced NAGase responses that were more similar to the TiO 2 than to the quartz dust response.…”

Section: Discussionsupporting

confidence: 50%

N-acetyl-beta-D-glucosaminidase activity within BAL from macaques exposed to generic coal dusts

Mack

Griffith

Riling

et al. 1995

Lung

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N-acetyl-beta(beta)-D-glucosaminidase is a lysosomal enzyme secreted by alveolar macrophages in response to phagocytosis of particulate material. Alveolar macrophages participate in the degradation and fibrosis of pulmonary tissue that results in pneumoconiosis. Known quantities of four characterized respirable dusts were bronchoscopically placed into the right caudal lung lobe of macaque monkeys. Bronchoalveolar lavage (BAL) samples were collected from dust-exposed right lung and unexposed left lung of the same individuals at 2-week intervals for 12 weeks after dust instillation. The samples were tested for N-acetyl-beta-D-glucosaminidase activity to determine if the enzyme levels could serve as an indicator of pulmonary injury induced by generic coal dusts when compared to known fibrogenic and nuisance dusts. Installation of generic quartz, anthracite, or TiO2 dusts produced significant elevations of enzyme activity and increased numbers of macrophages in the dust-exposed lobes. Elevations in enzymatic activity and macrophage numbers were greatest in response to generic quartz dust. These results suggest that quantitative levels of N-acetyl-beta-D-glucosaminidase activity may be a useful indicator of acute and chronic lung injury following exposure to fibrogenic and nonfibrogenic dusts.

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Section: Discussionsupporting

confidence: 50%

N-acetyl-beta-D-glucosaminidase activity within BAL from macaques exposed to generic coal dusts

Mack

Griffith

Riling

et al. 1995

Lung

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“…However, it may be due to the toxicity of the PM 10 particles and/or overload which would both restrict macrophage movement via the cytoskeleton (Möller et al 2002). Other pathogenic particles have been found to inhibit macrophage chemotactic activity following in vivo exposure (Donaldson et al 1990). Neutrophils closely situated to macrophages in the bronchoalveolar space during inflammation may release factor(s) that inhibit macrophage membrane-related functions such as phagocytosis, chemotaxis and spreading, and this may also provide an explanation (Donaldson et al 1988(Donaldson et al , 1989).…”

Section: Discussionmentioning

confidence: 98%

Reduced alveolar macrophage migration induced by acute ambient particle (PM10) exposure

et al. 2007

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Increased levels of particulate air pollution (PM10) have been implicated as a causal agent in pulmonary disease exacerbation and increased deaths from respiratory and cardiovascular disorders. The exact mechanism by which PM10 drives toxicity in the lung is still unknown, but studies have focused on inhibition of macrophage function and impaired alveolar clearance mechanisms. To assess the effects of PM10 on pulmonary macrophage clearance mechanisms ex vivo, Wistar rats were instilled with 125 or 250 microg of PM10 collected from the North Kensington, London. Control rats were instilled with sterile saline. The rats were sacrificed after 18 h and a bronchoalveolar lavage (BAL) was performed. Macrophages isolated from the BAL fluid were assessed for ability to migrate towards a positive chemoattractant (ZAS) ex vivo and to perform phagocytosis. Macrophages isolated from the PM10-exposed rats showed inhibition of potential to migrate. Macrophage phagocytic ability ex vivo was also significantly reduced by the presence of PM10 inside the cells. This study indicates that acute PM10 exposure diminishes macrophage motility and phagocytosis in a manner that could prove deleterious to particle clearance from the alveolar region of the lung. Decreased particle clearance promotes inflammation, and hence, warrants further investigation in relation to the effects of chronic PM10 exposure on macrophage clearance mechanisms and establishing the mechanisms behind decreased macrophage migration.

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“…The results of the present study revealed that exposure to cement dust, decreased the physiological activity of PMNs as measured by decreased CL response in cement mill workers. The previous studies by Scheuchenzuber et al [20], Hermanowicz et al [21], Donaldson et al [22] and Sliwinski et al [23] focused directly or indirectly on the phagocytic function and dust but none of them concerned the cement mill workers. In the present study, the phagocytic function of PMNs stimulated with OPZ was signifi cantly decreased in cement mill workers, compared to controls (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…They found a marked impairment of the chemotactic function of neutrophils stimulated with zymosan-activated serum in all the groups of workers exposed to organophosphate pesticides. Similarly, Donaldson et al [22] conducted a study on rats exposed to clouds of the pneumoconiotic dusts including quartz, coal-mine dust, and chrysotile asbestos for 8, 32, and 75 days. For comparison, the rats were also exposed to the non-pathogenic dust of titanium dioxide (TiO2).…”

Section: Cement Dust Exposure and Chemiluminescence Response O R I G mentioning

confidence: 99%

Effect of Cement Dust Exposure on Phagocytic Function of Polymorphonuclear Neutrophils in Cement Mill Workers

Meo¹,

Rasheed²,

Khan³

et al. 2008

International Journal of Occupational Medicine and Environmental Health

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Objectives: Exposure to cement dust can cause various occupational health problems due to its increasing incidence and long-term complications. However, the infl uence of cement dust on phagocytic function of polymorphonuclear neutrophils (PMNs), has not as yet been investigated. Therefore, the aim of the study was to measure the phagocytic activity of PMNs by assessing chemilumiscence (CL) response in cement mill workers and controls. Material and Methods: In this study, 50 volunteer males, aged 25-60 years, apparently healthy and nonsmoking, were randomly selected from among cement mill workers. These workers were further classifi ed into subgroups based on exposure duration of less than 10, 10-20, and more than 20 years. The controls were 50 healthy, nonsmoking, males who matched the study group with respect to age, height, weight, and socioeconomic status. The phagocytic function of PMNs, stimulated with opsonized zymosan, was determined by measuring CL response. Results: The fi ndings show a signifi cant decrease in phagocytic activity of PMNs [PMNs OPZ p < 0.005] in cement mill workers compared to controls. Conclusion: It is concluded that exposure to cement dust can impair the phagocytic function of PMNs which is refl ected in decreased chemiluminescence response.

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Impaired chemotactic responses of bronchoalveolar leukocytes in experimental pneumocniosis

Cited by 27 publications

References 18 publications

N-acetyl-beta-D-glucosaminidase activity within BAL from macaques exposed to generic coal dusts

N-acetyl-beta-D-glucosaminidase activity within BAL from macaques exposed to generic coal dusts

Reduced alveolar macrophage migration induced by acute ambient particle (PM10) exposure

Effect of Cement Dust Exposure on Phagocytic Function of Polymorphonuclear Neutrophils in Cement Mill Workers

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