Impaired insulin signaling pathway in ovarian follicles of cows with cystic ovarian disease

Hein, Gustavo Juan; Panzani, Carolina G.; Rodríguez, Fernanda Mariel; Salvetti, Natalia Raquel; Díaz, Pablo Uriel; Gareis, Natalia Carolina; Benítez, G.; Ortega, Hugo Héctor; Rey, Florencia

doi:10.1016/j.anireprosci.2015.02.010

Cited by 26 publications

(35 citation statements)

References 62 publications

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“…Insulin levels were below normal accepted reference values previously established. Our results are similar to those of Vanholder et al [32] and Hein et al [16] . In another study, Obese et al [38] found that insulin concentrations were higher in animals that had an early resumption of cyclicity compared to those who had a delay.…”

Section: Discussionsupporting

confidence: 93%

“…However, although the phenomenon of 'insulin resistance' is linked to the polycystic ovary syndrome associated with metabolic disorders including diabetes [39] , the authors were unable to demonstrate such relationship in the case of OC in cows [27] . More recently, Hein et al [16] observed low expression of insulin receptor (IR and IRS1) in cystic cows.…”

Section: Discussionmentioning

confidence: 99%

“…Insulin also stimulates expression of LH receptors in granulosa cells, and indirectly by stimulation of IGF-1 receptor [32] . Therefore, hypoinsulinemia may not only reduce the production of androgens and estradiol, but also alters the follicle's ability to acquire LH receptors, which compromises development and ovulation and promotes persistence of follicles as anovulatory structures [16] . Previous studies have revealed that insulin secretion is impaired even after the administration of glucose in cystic cows [27] .…”

Section: Discussionmentioning

confidence: 99%

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Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma

Mimoune¹,

Kaidi²,

Azzouz³

et al. 2017

Kafkas Univ Vet Fak Derg

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A clinical study was performed to evaluate the diagnostic methods for ovarian cysts (OC), and to determine the metabolic profiles of animals with OC in the region of Mitidja in the North of Algeria. A total of 504 non-pregnant lactating cows were used in this study. Ultrasonography was performed by EXAGO scanner and was combined with assessment of serum P4. Biochemical serum parameters were assayed by spectrophotometry and insulin and cortisol serum measurement was performed by electrochemiluminescence. The results showed an overall incidence of 11.9% of OC. The incidence of OC was higher among cows in third lactation. Holstein breed was the most affected by OC compared with other breeds (P<0.001). There were no effects of average BCS (Body Condition Scoring) and milk production on the incidence of OC (P>0.05). OC were single in 91% of cases. They were found mainly on the right ovary (66.66%). Seasonality had a significant influence on incidence rate of OC with higher incidence rates during winter and spring (71.66%); while, 28.33% of OC were detected during the summer and autumn (P<0.05). OC were associated with low serum concentrations of glucose, insulin and urea as well as high levels of cortisol. Ultrasound examination and progesterone assays were proposed as the most effective diagnostic combination to diagnose OC. In conclusion, in addition to hormonal imbalances, metabolic disorders are involved in the formation and/or persistence of OC. Therefore, the use of metabolic indicators in understanding and exploration of OC is of great interest. Keywords: Cow, Ovarian cyst, Metabolic profile, Ultrasonography Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma ÖzetOvaryum kistlerinin tanısında kullanılan metotları değerlendirmek ve Cazayir'in kuzeyindeki Mitidja bölgesindeki ovaryum kistli hayvanların metabolik profillerini belirlemek amacıyla klinik bir çalışma gerçekleştirildi. Toplam 504 gebe olmayan laktasyondaki inekler bu çalışmada kullanıldı. EXAGO tarayıcı kullanılarak ultrasonografi uygulandı ve serum P4 verileri ile birlikte değerlendirildi. Biyokimyasal serum parametreleri spektrofotometri ile belirlenirken insülin ve kortizol serum ölçümleri elektrokimyasal görüntüleme ile gerçekleştirildi. Ovaryum kist insidansı %11.9 olarak belirlendi. Ovaryum insidansı üçüncü laktasyondaki ineklerde daha fazla oranda tespit edildi. Diğer türler ile karşılaştırıldığında Holstein ırkında ovaryum kisti daha fazla oranda gözlemlendi (P<0.001). Ovaryum kisti üzerine Vücut Kondisyon Skorunun bir etkisi gözlemlenmedi (P>0.05). Ovaryum kisti, vakaların %91'inde tek taraflı olarak tespit edildi. Kistler çoğunlukla (%66.66) sağ ovaryumda yer almaktaydı. Mevsimin ovaryum kistlerinin ortaya çıkmasında önemli bir etkiye neden olduğu, kış ile ilkbahar aylarında (%71.66) insidansın arttığı yaz ve sonbaharda ise düşük seviyede (%28.33) olduğu belirlendi (P<0.05). Ovaryum kistlerinin düşük glukoz, insülin ve üre ile yüksek kortizol serum konsantrasyonları ile ilişkili olduğu belirlendi. Ult...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma

Mimoune¹,

Kaidi²,

Azzouz³

et al. 2017

Kafkas Univ Vet Fak Derg

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“…Variations in modulators of the PI3K signalling cascade have been observed (Hein et al. ); therefore, changes in regulators would be involved in the variations of IGFR1 levels detected in this work. On the other hand, some studies suggest that oestradiol can stimulate cell proliferation by activating IGFR1 ligand independently (Song et al.…”

Section: Discussionmentioning

confidence: 65%

Involvement of PAPP‐A and IGFR1 in Cystic Ovarian Disease in Cattle

Rodríguez

Colombero

Amweg

et al. 2015

Reprod Domestic Animals

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Cystic ovarian disease (COD) is one of the main causes of infertility in dairy cattle. It has been shown that intra-ovarian factors, such as members of the insulin-like growth factor (IGF) system, may contribute to follicular persistence. The bioavailability of IGF to initiate its response by binding to specific receptors (IGFRs) depends on interactions with related compounds, such as pregnancy-associated plasma protein A (PAPP-A). The aim of this study was to determine IGFR1 and PAPP-A expression both in follicles at different stages of development and in cysts, to evaluate the roles in the etiopathogenesis of COD in cattle. The mRNA expression of PAPP-A was higher in granulosa cells of large tertiary follicles than in cysts, whereas the protein PAPP-A present in the follicular fluid from these follicles showed no differences. Although no PAPP-A mRNA expression was detected in smaller tertiary follicles, in their follicular fluid, this protease was detected in lesser concentration than in cysts. The mRNA expression of IGFR1 was lower in granulosa cells from cystic follicles than in those from tertiary ones. However, the protein expression of this receptor presented the highest levels in cystic structures, probably to increase the possibility of IGF response. The data obtained would indicate that animals with COD have an altered regulation of the IGF system in the ovary, which could be involved in the pathogenesis of this disease in cattle.

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“…En folículos quísticos bovinos existe una mayor expresión de IGFR1 en células de la granulosa y niveles crecientes del mismo a medida que aumenta el tamaño del folículo (Lucy, 2008;Silva et al, 2009;. Por otro lado, hay variaciones en ciertos moduladores de la cascada de señalización PI3K (Hein et al, 2015). Esta modulación diferencial de los componentes de esta vía de señalización o la posibilidad de que PI3K sea reclutada y activada por hormonas o factores de crecimiento como el sistema IGF, podrían estar implicados en las variaciones de los niveles de IGFR1 detectados.…”

Section: Expresión De Los Diferentes Componentes Del Sistema Igf En Lunclassified

Participación del sistema IGF en la patogenia de la enfermedad quística bovina

Rodríguez¹,

Salvetti²,

Ortega³

et al. 2016

FAVE Cs Vet

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La enfermedad quística ovárica (COD) es una de las principales causas de falla reproductiva y de infertilidad, y constituye uno de los de los trastornos reproductivos más frecuentes en vacas lecheras de alta producción. El desarrollo de esta enfermedad está asociado a un desequilibrio en el eje hipotalámico-hipofisario-gonadal en el cual factores endocrinos, entre ellos el sistema de factores de crecimiento análogos a insulina (IGF), participan en el desarrollo folicular, diferenciación celular y la secreción de hormonas ováricas. Considerando al sistema IGF como un importante regulador del crecimiento y funcionalidad folicular, evaluamos la participación de componentes del sistema IGF en el desarrollo de la COD. Las alteraciones en diferentes componentes del sistema IGF que podrían afectar al normal funcionamiento del ovario participando en el desarrollo de la COD en bovinos.

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Impaired insulin signaling pathway in ovarian follicles of cows with cystic ovarian disease

Cited by 26 publications

References 62 publications

Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma

Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma

Involvement of PAPP‐A and IGFR1 in Cystic Ovarian Disease in Cattle

Participación del sistema IGF en la patogenia de la enfermedad quística bovina

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