Implantation of platelet rich fibrin and allogenic mesenchymal stem cells facilitate the healing of muscle injury: An experimental study on animal

Abstract

Introduction: Muscle injury has caused adverse impacts on athletes' performance. Muscle injury treatments are based on the degree of severity. Unfortunately, in extensive injuries, surgical treatments are often unsatisfactory especially in athletes with high functional demand. More effort is needed to achieve a better result in muscle injury healing. The use of platelet-rich fibrin (PRF) and mesenchymal stem cell (MSC) would provide all the necessary factors to achieve good tissue healing: cells, growth factors, and scaffold. The study aims to evaluate the role of PRF and MSC in facilitating the healing of muscle injury on animal models. Methods: A model defect was created in the gastrocnemius muscle of each hind leg of twenty New Zealand white rabbits. All legs were randomly divided into four groups: (1) control; (2) PRF-only; (3) MSC-only, and (4) PRF-and-MSC group. After two and four weeks, the muscle was retrieved and sent for immunohistochemistry examination to evaluate the expression of Pax7 and MyoD protein. Results: The mean score of all treated group was higher compared to the control group. The group that received both PRF and MSC showed the highest score. Conclusion: Considering the promising result, application of PRF and MSC could be an option for the treatment of muscle injury as this would provide all necessary elements of tissue engineering to facilitate the healing process of muscle: the cells, the scaffold, and the growth factors. Highlights:

PRF na harmonização orofacial: uma revisão narrativa da literatura

“…A ativação e a formação de coágulos de fibrina são provenientes do sangue. E a natureza é autóloga do PRF o que reduz o risco de reação imunogênica e transmissão de doenças (Utomo et al, 2018). O PRF libera fatores de crescimento por um período prolongado: até 7 dias para a maioria dos fatores de crescimento.…”

Tratamento da lesão muscular com células-tronco – Estudo experimental em coelhos

“…Next, partial injury in the 1/3 middle of the femoral rectus was performed ( Figure 2B ), with coldblade, and marking of the extremities ( Figure 2C ) with nylon 6-0 (Nylon 6-0, Shalon, Alto da Boa Vista, GO, Brazil), at approximately 0.5 cm proximal and distal to the lesion. 19 20 After performing the procedures and anesthetic recovery, the animal was encouraged to apply load to the limb, without restrictions.…”

“…Na sequência, foi realizada lesão parcial no 1/3 médio do reto femoral ( Figura 2B ), com lâmina fria, e marcação das extremidades ( Figura 2C ) com nylon 6-0 (Nylon 6-0, Shalon, Alto da Boa Vista, GO, Brazil), a uma distância aproximada de 0,5 cm proximal e distal à lesão. 19 20 Após a realização dos procedimentos e recuperação anestésica, o animal foi estimulado a aplicar carga no membro, sem restrições.…”

The Platelet Concentrates Therapy: From the Biased Past to the Anticipated Future

“…In addition, a new concept of "coupling therapy" has recently been proposed, which is based on a tissue engineering triangle [11,12] and suggests the need for stem cell replacement [13][14][15][16].…”