Implementación y evaluación de dos métodos deconservación y generación de la base de datos del banco de cepas y genes del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN)

Huertas, Sandra Liliana Parra; Casas, Maria Marcela Pérez; Morales, Mauricio Bernal; Moreno, Zulma Suárez; Castaño, Dolly Montoya

doi:10.22490/24629448.346

Cited by 3 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method of maintaining the fungi used in this study was periodic replanting, a technique that ensures good survival of the cultures over short periods of time. However, for greater reliability in the results, other methods for maintaining microbial strains should be sought, such as that recommended by Aleman et al (2005) and Huertas et al (2006), where the viability, purity and genetic stability of the cultures is guaranteed.…”

Section: Replication Of the Phytopathogenic Fungus E Salmonicolor Inmentioning

confidence: 99%

ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor

et al. 2017

View full text Add to dashboard Cite

In this work, a methodology of synthesis was designed to obtain ZnO nanoparticles (ZnO NPs) in a controlled and reproducible manner. The nanoparticles obtained were characterized using infrared spectroscopy, X-ray diffraction, and transmission electron microscopy (TEM). Also, we determined the antifungal capacity in vitro of zinc oxide nanoparticles synthesized, examining their action on Erythricium salmonicolor fungy causal of pink disease. To determine the effect of the quantity of zinc precursor used during ZnO NPs synthesis on the antifungal capacity, 0.1 and 0.15 M concentrations of zinc acetate were examined. To study the inactivation of the mycelial growth of the fungus, different concentrations of ZnO NPs of the two types of synthesized samples were used. The inhibitory effect on the growth of the fungus was determined by measuring the growth area as a function of time. The morphological change was observed with high-resolution optical microscopy (HROM), while TEM was used to observe changes in its ultrastructure. The results showed that a concentration of 9 mmol L -1 for the sample obtained from the 0.15 M and at 12 mmol L -1 for the 0.1 M system significantly inhibited growth of E. salmonicolor. In the HROM images a deformation was observed in the growth pattern: notable thinning of the fibers of the hyphae and a clumping tendency. The TEM images showed a liquefaction of the cytoplasmic content, making it less electron-dense, with the presence of a number of vacuoles and significant detachment of the cell wall.

show abstract

Section: Replication Of the Phytopathogenic Fungus E Salmonicolor Inmentioning

confidence: 99%

ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor

et al. 2017

View full text Add to dashboard Cite

show abstract

Viability, purity, and genetic stability of entomopathogenic fungi species using different preservation methods

et al. 2017

View full text Add to dashboard Cite

Métodos de conservación para actinobacterias con actividad solubilizadora de fósforo

Ortiz¹,

Prada²,

Franco-Correa³

2016

Rev.Colomb.Biotecnol.

View full text Add to dashboard Cite

El objetivo de esta investigación fue evaluar diferentes métodos de conservación para actinobacterias solubilizadoras de fósforo debido a la poca información de métodos específicos reportados para estos microorganismos. Los métodos de conservación se evaluaron a 3 diferentes periodos de tiempo; largo, mediano y corto plazo, usando métodos de congelación y liofilización; arcilla, sílica, arena y transferencia periódica, respectivamente. Para ello se usaron 15 aislamientos de 3 localidades diferentes (La Vega, Maní y Tota) y un banco de referencia de la Unidad de Investigaciones Agropecuarias de la Pontificia Universidad Javeriana. Se prepararon todos los inóculos en solución salina al 0,85% (p/v) y se ajustaron a una concentración de 108 cel/mL, seguido a ello se inocularon los viales de cada método de conservación con sus respectivos crioprotectantes, glicerol 20% (v/v), 30% (v/v) para congelación y skim milk 18% (p/v) para liofilización. Los métodos a mediano plazo se ejecutaron de igual manera, el inóculo se agregó a 10 perlas de arcilla, 10 g de arena y 5 g de sílica, posteriormente se almacenaron a 4ºC. El método de corto plazo se evaluó en agar avena (15 g/L). La evaluación se realizó mediante recuento directo en cámara de Neubauer por la técnica de azul de tripán, además de la caracterización macroscópica y microscópica de cada aislamiento en transferencia periódica. Se estableció que la actividad solubilizadora de fósforo se mantuvo más estable en los métodos de glicerol 30 % (p/v) y liofilización según el análisis estadístico. Palabras clave: viabilidad, crioprotectantes, banco, liofilización, crioconservación.

show abstract

Implementación y evaluación de dos métodos deconservación y generación de la base de datos del banco de cepas y genes del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN)

Cited by 3 publications

References 7 publications

ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor

ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor

Viability, purity, and genetic stability of entomopathogenic fungi species using different preservation methods

Métodos de conservación para actinobacterias con actividad solubilizadora de fósforo

Contact Info

Product

Resources

About