Implication of activation of intraperitoneal macrophages with bio-degradable microspheres

Abstract

To estimate the possibility that biological degradable starch microspheres (DMS) activate abdominal or intraperitoneal macrophages (IMP), two sizes of DMS (Spherex, Pharmacia, Sweden) were injected into the peritoneum of the ICR mice of 4 to 8 weeks of age. Three days after the injection, peritoneal fluid was collected and incubated for one hour at 37 degrees C under 5% CO2. The cells which adhered to the petri dish were IMP, to which DMS was added for 18 hrs. The cultured IMP were observed by scanning electron microscope (SEM) and the ratio of the active type to the total number of IMP was counted as an index of the effect of DMS to IMP. The activation effect of DMS on the incubated IMP was significant in the group which was cultured with 2 microns DMS after the 45 microns DMS injection. That indicated the possible DMS function as a potential IMP activating factor (MAF).

Nanomedicines for the treatment of inflammatory bowel diseases

“…In particular antigen presenting cells such as macrophages and dendritic cells actively engulf particulate carrier systems by endocytosis (97,98) . Microspheres prepared from biodegradable polymers and copolymers such as polylactic acid and polyglycolic acid (99, 100) and various starches and cross linked starches (101,102) are observed to be successfully internalized by macrophages, however the exact mechanism of phagocytosis is still not clearly understood (103) . Still, uptake by immune cells might significantly contribute to the drug carrier accumulation in the inflamed intestine and also provide a successful strategy to target key players in the inflammation process.…”

Photobiological and Ultrastructural Studies of Nanoparticles of Poly(lactic-co-glycolic acid)-Containing Bacteriochlorophyll-aas a Photosensitizer Useful for PDT Treatment

“…This behavior reduces the chances of toxicity commonly associated with cationic lipids and cationic polymers allowing their efficient intracellular delivery (Panyam et al 2002). At the same time macrophages and leukocytes are two of the most representative phagocyte cells acting in a biological system as a first defensive barrier to protect their host from invaders, by engulfing and digesting them very fast and efficiently (Okawa et al 1989;Ashsan et al 2002).…”