Importance of actin cytoskeleton behaviour during preservation of carrot cell suspensions in liquid nitrogen

Morisset, C.; Gazeau, Claude; Hansz, J; Dereuddre, J.

doi:10.1007/bf01378860

Cited by 8 publications

(2 citation statements)

References 39 publications

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“…Interestingly, in wheat the induction of an active ADF during cold acclimation is correlated with increased freezing tolerance (Ouellet et al, 2001). In addition, partial disruption of actin filaments may promote freezing tolerance during preservation of carrot cell suspensions in liquid nitrogen (Morisset et al, 1993). From the viewpoint of overwintering, the quick recovery of ER membrane dynamics after freeze-thawing is also important for plant cells to survive during winter, because it may enable the rapid recovery of many metabolic activities and, in early or late winter, there are many days in which the temperature increases above 0°C during the day.…”

Section: Discussionmentioning

confidence: 99%

Confocal cryomicroscopic analysis and cryodynamics of endoplasmic reticulum in herbaceous plant cells

Kobayashi

Kutsuna

Tanino

et al. 2014

Environmental and Experimental Botany

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Plants that survive subzero temperatures in winter enhance their freezing tolerance upon exposure to non-freezing temperature, a phenomenon termed cold acclimation. Although the mechanism of enhanced freezing tolerance has been widely examined at the molecular level, the understanding of cell physiological aspects remains poor. In our previous study, confocal cryomicroscopic observation of leaf epidermal cells of Allium fistulosum, the cold-hardy Welsh onion, showed that extracellular freezing seems to induce cessation of streaming and development of vesiculation of the endoplasmic reticulum (ER). Further analysis of this unique cryobehavior of ER may lead to a better understanding of how plant cells survive freezing. We confirmed that ER vesiculation occurred in root cells of Arabidopsis thaliana, which suggested that freeze-induced vesiculation of ER might be conserved in herbaceous plants. Because the ER membrane dynamics in Welsh onion cells were more clearly observed than those in Arabidopsis cells, the Welsh onion system was used for more detailed analyses. Average velocities of ER membranes were quantitatively analyzed and resultant Arrhenius plots showed that extracellular freezing rapidly decreased ER streaming, which had ceased completely at −5°C in both non-acclimated and cold-acclimated cells. After thawing, only in cold-acclimated cells, ER networks quickly recovered at 2°C. Furthermore, during supercooling at −5°C, only ER of cold-acclimated cells maintained streaming and networks. These results indicated that ER networks in cold-acclimated cells are more stable at low temperature, and extracellular freezing results in the breakdown of cold-stable ER networks in cold-acclimated cells. One possibility is that the breakdown of ER networks is involved in an enhanced freezing tolerance mechanism.

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Section: Discussionmentioning

confidence: 99%

Confocal cryomicroscopic analysis and cryodynamics of endoplasmic reticulum in herbaceous plant cells

Kobayashi

Kutsuna

Tanino

et al. 2014

Environmental and Experimental Botany

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show abstract

“…Finally, histological and ultrastructural investigations may give insight into damage and repair mechanisms and pathways of regeneration [23]. Changes in the cytoskeleton may be of value in this respect [24]. Several other methods have been used to investigate freezing and thawing processes, including nuclear magnetic resonance [25] and cryomicroscopy [26].…”

Section: 21 Main Principlesmentioning

confidence: 99%