Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus

Khan, Imran Ali; Vattam, Kiran Kumar; Jahan, Parveen; Hasan, Qurratulain; Rao, Pragna

doi:10.5582/irdr.2015.01040

Cited by 20 publications

(13 citation statements)

References 25 publications

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“…The age of students has been calculated with Years and weight was measured with kilograms (kgs) and height was measured with centimeters or meter square (cms/m 2 ). Body mass Index (BMI) was calculated with formula BMI=weight (kg)/height (cms/m 2 ) 14 ) . The below normal weight (<18.5 kg/m 2 ), normal weight (18.5–24.9 kg/m 2 ), overweight (25.0–29.9 kg/m 2 ), obese (30.0–39.9 kg/m 2 ), and extremely obese (40 kg/m 2 ) was opted as per the World Health Organization criteria for BMI in our study.…”

Section: Methodsmentioning

confidence: 99%

Correlation between obesity and sleep disturbance in Saudi medical students

et al. 2017

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[Purpose] The aim of the present study was to investigate the association between sleep duration, quality and obesity in the medical students of Saudi population. [Subjects and Methods] This is a cross-sectional study carried out in 408 medical students from King Khalid University Hospitals, Riyadh, Saudi Arabia, based on well-designed questionnaire study and consent form. [Results] The results of this study revealed 39.9% of subjects perform the exercise on regular basis and 61.5% of them reduced their weight, which may be due to diet plan (52.9%) also. Only 68.6% of the subjects were affected with the weight. The maximum number of subjects was lying down after the lunch (88.2%), sitting and reading (80.1%) are prone to develop obesity in future. [Conclusion] Our study confirms sleep disturbance has affected the weight gain and the maximum number of subjects was lying down after the lunch and sitting and reading will be prone to develop obesity in future.

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Section: Methodsmentioning

confidence: 99%

Correlation between obesity and sleep disturbance in Saudi medical students

et al. 2017

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“…Weight (kg)/height (m 2 ) metrics were used to calculate body mass index (BMI). Serum samples were used to measure fasting blood sugar (FBS), postprandial blood glucose (PPBS), glycosylated hemoglobin (HbA1C) and lipid profile; total cholesterol (TC), triglycerides (TG), high‐density‐lipoprotein‐cholesterol (HDL‐C) and low‐density lipoprotein‐cholesterol (LDL‐C) . Homeostasis model assessment for insulin resistance (HOMA–IR) was calculated to evaluate IR status using the formula; glucose (mmol/L) X insulin (lIU/mL)/22.5.…”

Section: Methodsmentioning

confidence: 99%

Distribution of CYP2C8 and CYP2C9 amino acid substitution alleles in South Indian diabetes patients: A genotypic and computational protein phenotype study

Rao

Murthy

Shaik

et al. 2017

Clin Exp Pharma Physio

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The CYP2C8 and CYP2C9 are two major isoforms of the cytochrome P450 enzyme family, which is involved in drug response, detoxification, and disease development. This study describes the differential distribution of amino acid substitution variants of CYP2C8 (*2-I269F & *3-R139K) and CYP2C9 (*2-C144R & *3-L359A) genes in 234 type 2 diabetes mellitus (T2DM) patients and 218 healthy controls from Andhra Pradesh, South India. Single locus genotype analysis has revealed that homozygous recessive genotypes of 2C8*2-TT (P ≤ .03), 2C9*2-TT (P ≤ .02), and heterozygous 2C9*3-AC (P ≤ .006) are seen to be increasingly present in the case group, indicating a significant level of their association with diabetes in Andhra population. The statistical significance of these recessive genotypes has persisted even under their corresponding allelic forms (P ≤ .01). Genotype association results were further examined by computational protein structure and stability analysis to assess the deleteriousness of the amino acid changes. The mutant CYP 2C8 and 2C9 (both *2 and *3) proteins showed structural drifts at both amino acid residue (range 0.43Å-0.77Å), and polypeptide chain levels (range 0.68Å-1.81Å) compared to their wild-type counterparts. Furthermore, the free energy value differences (range -0.915 to -1.38 Kcal/mol) between mutant and native protein structures suggests the deleterious and destabilizing potential of amino acid substitution polymorphisms of CYP genes. The present study confirms the variable distribution of CYP2C8 (*2 and *3) and CYP2C9 (*2 and *3) allelic polymorphisms among South Indian diabetic populations and further warrants the serious attention of CYP gene family, as a putative locus for disease risk assessment and therapy.

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“…Gene identification is important for understanding the disease pathophysiology and refining prevention, diagnosis, and treatment. Genetic polymorphisms are confirmed through multiple alleles at distinct DNA loci (Hanson et al, 2017;Khan et al, 2016), and human phenotypic variants occur due to genetic and environmental factors (Fucharoen, 2007). A combination of human genome sequencing and single nucleotide polymorphism (SNP) has provided the required amount of genetic data for human diseases (Romero-Sánchez et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Role of C677T polymorphism in the MTHFR gene in Saudi females affected with infertility

Al-Mutawa¹

2019

Int. j. adv. appl. sci.

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The methylenetetrahydrofolate reductase (MTHFR) gene plays a major role in folate metabolism in infertile women. To date, limited studies have implicated the C677T polymorphism in MTHFR with female infertility, which is defined as the failure to accomplish clinical pregnancy with consistent unprotected sexual intercourse for 1 year; this disorder is influenced by various factors, including obesity and it affects in ~15% of couples during the reproductive period. However, limited molecular studies have been conducted pertaining to female infertility. The current study aimed to investigate the correlation between the C677T polymorphism in MTHFR and infertility in a Saudi population. In this study, 300 genomic DNA samples were extracted from 150 infertile and 150 fertile women, and genotyping was performed for the C677T polymorphism in MTHFR by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The results revealed that the mutant T allele was significantly associated with infertility (odds ratio [OR] = 1.86; 95% CI = 1.15-2.99; p = 0.009). Additionally, the dominant and recessive modes of inheritance were positively associated with infertility, i.e., TT vs. CT+CC (OR = 2.98; 95% CI = 1.33-6.66; p = 0.005) and TT+CT vs. CC (OR = 1.89; 95% CI = 1.09-3.26; p = 0.02). The current results indicate that the T allele of the C677T polymorphism in MTHFR is a significant risk factor for infertility in women from a Saudi population.

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Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus

Cited by 20 publications

References 25 publications

Correlation between obesity and sleep disturbance in Saudi medical students

Correlation between obesity and sleep disturbance in Saudi medical students

Distribution of CYP2C8 and CYP2C9 amino acid substitution alleles in South Indian diabetes patients: A genotypic and computational protein phenotype study

Role of C677T polymorphism in the MTHFR gene in Saudi females affected with infertility

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