Importance of Understanding the Main Metabolic Regulation in Response to the Specific Pathway Mutation for Metabolic Engineering of Escherichia Coli

Abstract

Recent metabolic engineering practice was briefly reviewed in particular for the useful metabolite production such as natural products and biofuel productions. With the emphasis on systems biology approach, the metabolic regulation of the main metabolic pathways in E. coli was discussed from the points of view of enzyme level (allosteric and phosphorylation/ dephosphorylation) regulation, and gene level (transcriptional) regulation. Then the effects of the specific pathway gene knockout such as pts, pgi, zwf, gnd, pyk, ppc, pckA, lpdA, pfl gene knockout on the metabolism in E. coli were overviewed from the systems biology point of view with possible application for strain improvement point.

A New Insight into the Main Metabolic Regulation of Escherichia coli Based on Systems Biology Approach

“…While the specific-gene knockout mutations preclude growth on glucose, a majority of such mutations seem to be potentially compensated by the use of alternative enzymes or by rerouting of the carbon fluxes through alternative pathways, resulting in a robust phenotype such as little effect on the cell growth rate etc. (Matsuoka and Shimizu, 2012).…”

Knowledge discovery from data: InterCriteria Analysis of mutation rate influence

“…An E. coli fed-batch fermentation model is considered as a case study. This process has been chosen as a representative of some of the important microorganisms with numerous applications in food and pharmaceutical industry, as well as one of the widely used model organisms in genetic engineering and cell biology, due to their well known metabolic pathways [13].…”

Shikimic Acid Production in Escherichia coli: From Classical Metabolic Engineering Strategies to Omics Applied to Improve Its Production

“…However, despite the great achievements accomplished through this discipline, performance improvement has become limited after the first breakthroughs, mainly because of the traditional local pathway modification strategies. This is probably due to the limited understanding of the overall mechanism of metabolic regulation (Matsuoka and Shimizu, 2012 ). Therefore, given the importance of finding not only a particular pathway but also global information regarding cell physiology and metabolism to overcome production limitations, a systems biology approach supported by omics data may be the solution for improving SA production.…”

“…Metabolic engineering has been used since 1991 for strain modification by using recombinant DNA technology to enhance the production of specific metabolites (Matsuoka and Shimizu, 2012 ). The efforts to use ME have extended from the early years to optimize many cellular behaviors or parameters, such as substrate consumption, robustness, and tolerance toward toxic compounds and media conditions (Matsuoka and Shimizu, 2012 ).…”