Impression cytology diagnosis of ulcerative eyelid malignancy

Sen, Seema; Lyngdoh, A. D.; Pushker, Neelam; Meel, Rachna; Bajaj, Mandeep S; Chawla, Bhavna

doi:10.1111/cyt.12133

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…ICS and CBS provided samples with equal cell quality in healthy and diseased horses. In human ophthalmology, ICS is used for the diagnosis of ocular surface tumours as cells harvested with ICS are of highest quality [4,9,10,25]. In the current study, the 2 cases of SCC were identified with both ICS and CBS.…”

Section: Discussionmentioning

confidence: 90%

Impression cytology as diagnostic tool in horses with and without ocular surface disease

Braus

Lehenauer

Tichy

et al. 2016

Equine Veterinary Journal

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Summary Background Cytology plays a major role in the diagnosis of ocular surface diseases. Objective To compare 2 cytological sampling methods for obtaining corneal and conjunctival cell samples regarding irritation for the patient, invasiveness, sample quality and diagnostic equivalence. Study design Observational prospective study. Methods In 5 healthy horses, conjunctival and corneal samples were taken bilaterally by impression cytology sampling (ICS) and cytobrush sampling (CBS). Irritation and invasiveness were assessed with an eye irritation and an epithelial damaging score system, respectively. Sample quality was evaluated via morphometric analysis and graded by a board certified clinical pathologist. For the assessment of diagnostic equivalence, 15 eyes of 14 client owned horses with ocular surface anomalies were sampled by ICS and CBS. The methods were compared regarding the types of inflammatory cells and/or infectious agents detected and if the correct diagnosis could be achieved. Histopathology served as gold standard when available. Results ICS was significantly less invasive and less irritating for the horses. Both methods retrieved cells of overall high quality; the cell quantity was significantly higher in IC samples. ICS preserved the natural cellular layout. There was a fair, but no statistically significant agreement between the diagnostic outcomes between sampling methods although CBS resulted in a slightly greater variability of inflammatory cell types compared to ICS. Main limitations None. Conclusions Because of its low irritability and invasiveness ICS can be recommended for cell sampling in fragile corneas and for experimental studies. ICS is especially useful in cases where preservation of cellular layout is advantageous. CBS samples are easier to obtain because of the small equine palpebral fissure. Additionally, the identification of inflammatory cells within conjunctival cell samples is easier. Overall, CBS is still an appropriate method in clinical settings.

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Section: Discussionmentioning

confidence: 90%

Impression cytology as diagnostic tool in horses with and without ocular surface disease

Braus

Lehenauer

Tichy

et al. 2016

Equine Veterinary Journal

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“…This limitation was already reported in human medicine when the diagnostic accuracy in detecting ocular neoplasia was compared between IC and histopathology. There was a high positive predictive accuracy, but only a fair negative predictive value of IC in identifying ocular surface neoplasia in one study, and another described a good sensitivity in detecting the presence of a neoplastic cells, but a low sensitivity in identifying the type of malignancy in eyelids . In all these studies, the main limitation identified for the IC was the loss of clarity of cytologic details.…”

Section: Discussionmentioning

confidence: 97%

Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique

Perazzi

Bonsembiante

Gelain

et al. 2017

Veterinary Clinical Pathol

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“…Because of its low sensitivity in terms of cytological categorization of the type of malignancy, IC cannot be recommended in the primary diagnosis of eyelid malignancies. Nevertheless, with experience and improvement in the technique, it may prove to be a useful tool in deciding future management, particularly in recurrences of histopathologically confirmed eyelid malignancies, where biopsies may be avoided (36) .…”

Section: Application Of Ic In the Evaluation Of Ulcerative Eyelid Malmentioning

confidence: 99%

Impression cytology in the evaluation of ocular surface tumors: review article

Barros¹,

Almeida²,

Lowen³

et al. 2015

Arquivos Brasileiros De Oftalmologia

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Impression cytology (IC) has been widely used as a method for evaluating the ocular surface and superficial cells layers in the diagnosis and follow-up after treatment of several ocular surface tumors of both epithelial and melanocytic origin. Information regarding this can be found in the English-language literature since 1992. Using either cellulose acetate or Biopore membranes for specimen collection, a high correlation has been found between IC and tissue histology. Compared with exfoliative cytology with spatula, IC is less traumatic to the patient's eye, provides a precise location of the area being studied, and allows accurate observation of the cells the way they exist in vivo. The additional advantage of IC is the preservation of limbal stem cells responsible for continuous corneal epithelium renewal; these can be affected after incisional or excisional biopsy at the corneoscleral limbus, which is the most frequent site of appearance of tumors in the stratified epithelium. Treatment for ocular surface squamous neoplasia has historically included surgery, but nonsurgical interventions have also been adopted. Hence, in certain cases, ophthalmologists may prefer interventions less invasive than surgical biopsy such as of impression cytology for both initial diagnosis and therapeutic monitoring of treatment for ocular surface lesions. Nevertheless, it should be considered that IC may be less helpful if the results conflict with the clinical picture or if the clinical diagnosis is uncertain and results are negative. In such cases, surgical biopsy is required for accurate diagnosis. The purpose of this review is to examine the published literature on the utilization of IC for the diagnosis and management of ocular surface tumors and to discuss the requirement for further investigation on the subject.

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Impression cytology diagnosis of ulcerative eyelid malignancy

Abstract: Impression cytology is a simple, useful, non-invasive technique for the detection of malignant ulcerative eyelid lesions. It is especially useful as a follow-up technique for the detection of recurrences.

Cited by 7 publications

References 19 publications

Impression cytology as diagnostic tool in horses with and without ocular surface disease

Impression cytology as diagnostic tool in horses with and without ocular surface disease

Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique

Impression cytology in the evaluation of ocular surface tumors: review article

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