Imprinted Polymers

Hall, Andrew J.; Emgenbroich, Marco; Sellergren, Börje

doi:10.1007/b104333

Cited by 26 publications

(7 citation statements)

References 70 publications

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“…The most promising non-antibody binders worth investigating in their role for PSP toxin analysis by SPR are chemosensors (Bell and Hext 2004), molecular imprinted polymers (MIPs) (Hall et al 2005) and aptamers (Tombelli et al 2005). The advantages and disadvantages of these particular binders are highlighted in Table 4.…”

Section: Potential Binders For Future Optical Spr Analysis Of Psp Toxinsmentioning

confidence: 99%

“…A wide range of non-antibody-based binders have been reviewed previously as alternative molecules for the detection of pathogens ( Ngundi et al 2006 ). The most promising non-antibody binders worth investigating in their role for PSP toxin analysis by SPR are chemosensors ( Bell and Hext 2004 ), molecular imprinted polymers (MIPs) ( Hall et al 2005 ) and aptamers ( Tombelli et al 2005 ). The advantages and disadvantages of these particular binders are highlighted in Table 4 .…”

Section: Potential Binders For Future Optical Spr Analysis Of Psp Toxmentioning

confidence: 99%

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Paralytic shellfish poisoning (PSP) toxin binders for optical biosensor technology: problems and possibilities for the future: a review

Campbell

Rawn

Niedzwiadek

et al. 2011

Food Additives & Contaminants: Part A

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This review examines the developments in optical biosensor technology, which uses the phenomenon of surface plasmon resonance, for the detection of paralytic shellfish poisoning (PSP) toxins. Optical biosensor technology measures the competitive biomolecular interaction of a specific biological recognition element or binder with a target toxin immobilised onto a sensor chip surface against toxin in a sample. Different binders such as receptors and antibodies previously employed in functional and immunological assays have been assessed. Highlighted are the difficulties in detecting this range of low molecular weight toxins, with analogues differing at four chemical substitution sites, using a single binder. The complications that arise with the toxicity factors of each toxin relative to the parent compound, saxitoxin, for the measurement of total toxicity relative to the mouse bioassay are also considered. For antibodies, the cross-reactivity profile does not always correlate to toxic potency, but rather to the toxin structure to which it was produced. Restrictions and availability of the toxins makes alternative chemical strategies for the synthesis of protein conjugate derivatives for antibody production a difficult task. However, when two antibodies with different cross-reactivity profiles are employed, with a toxin chip surface generic to both antibodies, it was demonstrated that the cross-reactivity profile of each could be combined into a single-assay format. Difficulties with receptors for optical biosensor analysis of low molecular weight compounds are discussed, as are the potential of alternative non-antibody-based binders for future assay development in this area.

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Section: Potential Binders For Future Optical Spr Analysis Of Psp Toxinsmentioning

confidence: 99%

Section: Potential Binders For Future Optical Spr Analysis Of Psp Toxmentioning

confidence: 99%

Paralytic shellfish poisoning (PSP) toxin binders for optical biosensor technology: problems and possibilities for the future: a review

Campbell

Rawn

Niedzwiadek

et al. 2011

Food Additives & Contaminants: Part A

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show abstract

“…A particular problem refers to targets which are either very polar and watersoluble or nonpolar poorly functionalized. Host-guest chemistry [6] and covalent imprinting approaches [7] can be productively used to address these problems. Computational techniques [8] and combinatorial imprinting [9] are other important tools for improving performance.…”

Section: Molecular Imprinting In Separation Sciencementioning

confidence: 99%

Molecular imprinting in Separation Science

Greibrokk¹,

Sellergren²

2009

J of Separation Science

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“…Molecular imprinting technology is one of the promising methods for synthesising functionalised material . The molecularly imprinted polymers (MIPs) thus prepared exhibit high selectivity, physico‐chemical stability and applicability in harsh chemical media, and have been applied in separation processes and chemical sensors . Use as an artificial antibody in immunoassay‐like analysis is one of the most interesting and exciting applications of MIPs, and some MIP‐based immunoassay methods have been reported .…”

Section: Introductionmentioning

confidence: 99%