Improved adhesion and growth of human osteoblast-like MG 63 cells on biomaterials modified with carbon nanoparticles

Bačáková, Lucie; Grausova, Lubica; Vacı́k, J.; Frączek, A.; Błażewicz, S.; Kromka, Alexander; Vaněček, M.; Švorčı́k, Václav

doi:10.1016/j.diamond.2007.07.015

Cited by 93 publications

(69 citation statements)

References 19 publications

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“…% of MWCNT, as measured by surface profilometry (T500 Hommel Tester, Hommelwerke Co., Germany). The microscale roughness was most probably due to clustering of carbon nanotubes and to prominences of these microclusters on the material surface (Bacakova et al, 2007b(Bacakova et al, , 2008a). An increase in the microscale surface roughness in polymer-carbon nanotube composites has been considered as a factor decreasing the colonization of the composites with cells.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

“…In our earlier studies (Bacakova et al, 2007b;2008a), carbon nanotubes were used for the creation of composite materials by their mixing with terpolymer of polytetrafluoroethylene, polyvinyldifluoride and polypropylene (PTFE/PVDF/PP) or polysulfone (PSU), i.e., polymers which have been considered to be promising for the construction of bone implants. For example, PTFE/PVDF/PP has been tested as a material for septal nasal reconstruction (Scierski et al, 2007).…”

Section: Carbon Nanotubesmentioning

confidence: 99%

“…The suspensions were then exposed to ultrasound in a PARMER INSTRUMENTS sonicator (model CP 130PB, power 130W, frequency 20 kHz) for 10 minutes at 20 o C in order to prevent clustering of the nanoparticles. Finally, the suspensions were poured on to Petri dishes and left to evaporate the solvent (Bacakova et al, 2007b(Bacakova et al, , 2008a. The addition of SWCNH or MWCNT to the PTFE/PVDF/PP terpolymer markedly improved several parameters of the adhesion and growth of human osteoblast-like MG 63 cells (Bacakova et al, 2007b(Bacakova et al, , 2008a.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

“…Finally, the suspensions were poured on to Petri dishes and left to evaporate the solvent (Bacakova et al, 2007b(Bacakova et al, , 2008a. The addition of SWCNH or MWCNT to the PTFE/PVDF/PP terpolymer markedly improved several parameters of the adhesion and growth of human osteoblast-like MG 63 cells (Bacakova et al, 2007b(Bacakova et al, , 2008a. They were well-spread, polygonal, and contained distinct beta-actin filament bundles, whereas most cells on the pure terpolymer were less spread or even round and clustered into aggregates (Fig.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

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Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

Bačáková¹,

Grausova²,

Vacı́k³

et al. 2011

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Section: Carbon Nanotubesmentioning

confidence: 99%

Section: Carbon Nanotubesmentioning

confidence: 99%

Section: Carbon Nanotubesmentioning

confidence: 99%

Section: Carbon Nanotubesmentioning

confidence: 99%

See 2 more Smart Citations

Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

Bačáková¹,

Grausova²,

Vacı́k³

et al. 2011

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“…Nanocrystalline diamond possesses promising electrical and optical properties, high hardness, low friction coefficient and good compatibility (Bacakova et al, 2007). Nanocrystalline diamond has been used in the form of films to improve the mechanical and physical properties of body implants.…”

Section: Nanosurfacesmentioning

confidence: 99%

Novel Promises of Nanotechnology for Tissue Regeneration

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2012

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Diamond Seeding and Growth of Hierarchically Structured Films for Tissue Engineering

et al. 2009

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The growth of nanocrystalline diamond (NCD) has attracted many researchers because of the combination of advanced intrinsic properties such as their smooth surface, excellent optical, mechanical, and thermal properties, biocompatibility, high affinity for covalent bonding with specific organic molecules, and chemical inertness. [1][2][3] Low-cost insulating (nominally undoped) and semiconducting (boron-doped) nanocrystalline diamond films can be deposited on large areas [4] and thus new fields for their bioelectronic and biosensor applications are opened. [5][6][7][8][9] Moreover, in vivo studies of diamond-based implants (TiAl6V4 probes covered with NCD) in a rabbit femur showed very high bonding strength to the metal base and also to the surrounding bone tissue, without any problems of corrosion. [10] The large-area deposition of NCD films is still not a trivial technological task because of the low reproducibility over large areas and low nucleation yield. The main drawback of the low nucleation yield is the prolonged deposition time required for getting fully closed and uniform NCD layers.Common minimum film thickness is at least several hundred nanometers. There are several factors that can positively enhance the diamond growth, such as surface defects (scratching lines), the tendency of the substrate to form a carbide layer, the resistance of the substrate to carbon bulk diffusion, and the surface energy of substrate. [11] In comparison with several nucleation procedures, [12] the ultrasonic seeding technique with ultradispersed detonation diamond (UDD) powder seems to be the most promising technique to achieve extremely high nucleation yields. [13][14][15] However, detailed studies on ultrasonic seeding are mostly restricted to ''after'' growth study. [16][17] No details of the surface morphology of ''just seeded'' substrate or technological data (such as the UDD type and seeding time) have yet been published.Well-defined uniformity and controllable morphology of NCD films is of utmost importance for their biological applications in tissue engineering. Generally, the biological performance of a biomaterial is strongly dependent on its surface properties. [18] Surface energy, charge, wettability, chemistry, and topography influence the biological response of cells and tissues to an implant device. The first contact of cells with the implant surfaces is a multistep event. Firstly, serum/plasma proteins adsorb on the material surface, then cells attach to this protein layer, and integrins (specific cellular receptors), recognizing the presented extracellular ligands, mediate the first interaction of cells with the material. The attachment/adhesion process of the cells is accompanied by rearrangement of cytoskeleton proteins, formation of tight focal adhesion contacts, activation of focal adhesion kinase, and induction of several intracellular signal transduction pathways leading to cell proliferation and differentiation. [19] In that way the material surface indirectly determines cellular fate, finally the o...

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Improved adhesion and growth of human osteoblast-like MG 63 cells on biomaterials modified with carbon nanoparticles

Cited by 93 publications

References 19 publications

Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

Novel Promises of Nanotechnology for Tissue Regeneration

Diamond Seeding and Growth of Hierarchically Structured Films for Tissue Engineering

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