Improved endothelial cell attachment on ePTFE vascular grafts pretreated with synthetic RGD-containing peptides

Walluscheck, Knut P.; Steinhoff, Gustav; Kelm, Sørge; Haverich, Axel

doi:10.1016/s1078-5884(96)80251-6

Cited by 145 publications

(106 citation statements)

References 46 publications

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“…Overall, fibronectin has also been shown to have much better cellular attachment and retention than uncoated grafts ofePTFE, dacron and MyoLink (GIUDICEANDREA et al, 1998;KENT et al, 1992;KESLER et al, 1986;NEWMAN et al, 1991;SCOTT and MANN, 1990;WALLUSCHECK et al, 1996a). in comparative studies, FN shows enhanced cell attachment in comparison with collagen (BUDDet al, 1989;THOMSON et al, 1989), gelatin (BUDDet aL, 1989;IVARSSON et al, 1989;THOMSON et aL, 1989), poly-l-lysin (BUDD et al, 1989), laminin, albumin and gelatin (HASSON et aL, 1986;PRONK et al, 1994;THOMSON et al, 1989).…”

Section: Chemical Coatingsmentioning

confidence: 99%

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

Salacinski

Tiwari

Hamilton

et al. 2001

Med. Biol. Eng. Comput.

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Surgical treatment of vascular disease has become common. The use of synthetic materials is limited to grafts larger than 5-6mm, because of the frequency of occlusion observed with small-diameter prosthetics. An alternative would be a hybrid or tissue-engineered graft with the surface coated with a monolayer of the patient's own cells. Currently, to be effective, high-density seeding regimens have to be undertaken. This is because endothelial cells (ECs) are washed off the graft lumen once exposed to physiological blood flow. EC attachment has been shown to be significantly improved by pre-coating with substances known to attach ECs selectively. The review examines the various types of coating and bonding technology used to date to enhance endothelial cell attachment onto the surface of prosthetic vascular bypass grafts.

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Section: Chemical Coatingsmentioning

confidence: 99%

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

Salacinski

Tiwari

Hamilton

et al. 2001

Med. Biol. Eng. Comput.

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“…However, the adsorption and adhesion of the protein on unmodified ePTFE is limited due to interfacial characteristics. Another approach focuses on the modification of graft surfaces with domains in naturally occurring extracellular matrix (ECM) proteins, especially the Arginine-GlycineAspartic Acid (RGD) sequence, for specific interactions with integrin cell surface receptors (Walluscheck et al 1996;Elloumi et al 2006;Larson et al 2006). RGD peptides immobilized directly onto many materials have demonstrated enhanced EC attachment.…”

Section: The Cell Attachment Peptidementioning

confidence: 99%

Vascular Engineering to Make Blood-Compatible Surface

Ujiié

Suzuki

Liepsch³

2016

Vascular Engineering

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In spite of tremendous efforts in the last several decades, no artificial grafts have provided a satisfactory patency rate as small-caliber vascular alternatives (less than 5 mm diameter) in clinical applications. Owing to thrombus formation at acute stage and intimal thickening caused by compliance mismatch, the longterm patency of these small-caliber vascular grafts is still disappointing. Endothelial cell seeding has been proposed to improve the blood compatibility of small-diameter vascular grafts by creating an inner lining with similar non-thrombogenic surface characteristics as native blood vessels. To obtain consistent and firm endothelial cell linings to make hybrid vascular grafts, we review special surface modification technique ion beam implantation.

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“…It was postulated that endothelial cell seeding could eliminate thrombogenicity of tissue-engineered CV implants [25]. For superior endothelial engraftment, different in vitro cellularization techniques were introduced including bioreactor-driven conditionings [26,27,28,29] or direct coating strategies [30]. For the enhancement of the angiogenic microenvironment, autologous biomaterials like fibrin could be used in cell-plus-matrix molded or coated CV devices [30,31].…”

Section: Developments In Cell-plus-matrix Constructsmentioning

confidence: 99%

“…As an alternative, tissue-engineered CV implants could be cellularized in vivo (i.e. in situ) with possible regulation by cytokines or special surface coating strategies [29,32,33]. The incorporation of local drug delivery systems into the matrix of a graft could remarkably modulate in vivo stem cell recruitment and the recellularization process [32].…”

Section: Developments In Cell-plus-matrix Constructsmentioning

confidence: 99%

Tissue-Engineered Devices in Cardiovascular Surgery

Steinhoff¹

2012

Eur Surg Res

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Manufacturing life-long functional cardiovascular (CV) implants is the ultimate goal for researchers and clinicians in the cardiothoracic field. Tissue engineering (TE) is an opportunity to create ideal prostheses that are vital, growing, adaptive, autologous and functionally optimally performing. Today, initial translation from basic science to first clinical trials has begun. The article depicts the state of the art in TE techniques for CV products and describes milestones in the ongoing development of tissue-engineered myocardial, valvular and vascular devices from an experimental and clinical point of view. Artificial CV implants still reveal remarkable limitations but promising advances regarding optimal structural design, the prevention of intimal hyperplasia and the reduction of antigenicity and thrombogenicity. Where applicable, the implantation of vascularized autografts should still be preferred. Apart from that, decellularized allogen bioprostheses currently represent most promising matrix scaffolds that can be autologously cellularized in vitro prior to or in vivo after implantation. Capable biologic alternatives have been described like the decellularized porcine small intestinal submucosa. Rising evidence suggests that in vitro endothelialization might be the minimal requirement for improved long-term results of biological tissue-engineered CV grafts.

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Improved endothelial cell attachment on ePTFE vascular grafts pretreated with synthetic RGD-containing peptides

Abstract: EC attachment on uncoated ePTFE vascular prostheses is very weak. Our technique of coupling the ePTFE graft surface with cell adhesion promoting RGD-containing synthetic peptides significantly improved this decisive step in endothelial cell seeding of ePTFE grafts.

Cited by 145 publications

References 46 publications

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

Vascular Engineering to Make Blood-Compatible Surface

Tissue-Engineered Devices in Cardiovascular Surgery

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