Improved functionality of antibody-colloidal gold conjugates with the aid of lipoamide-grafted N-[tris(hydroxymethyl)methyl]acrylamide polymers

Albers, Willem M.; Munter, Tony; Laaksonen, Päivi; Vikholm-Lundin, Inger

doi:10.1016/j.jcis.2010.03.059

Cited by 18 publications

(20 citation statements)

References 33 publications

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“…The Lipa-pTHMMAA polymer forms a monolayer with a surface coverage of 460 ± 20 ng/cm 2 and the height of the layer is about 4 nm as measured by SPR [28]. The surface coverage of the polymer without lipoic acid is much lower: in the range of 200 ng/cm 2 with a height about half of the Lipa-pTHMMAA polymer.…”

Section: Monolayer Formation Of Polyacrylamide and Polyethylene Glycolmentioning

confidence: 91%

“…Poly(ethylene glycol) with a disulphide linkage (P8735-EG-disulfide, Mw 6500 g/mol), hereafter named EG-S-S, was from Polymer Source Inc. Quebec, Canada (Scheme 1c). The polymers pTHMMAA and Lipa-pTHMMAA were prepared according to the method by Albers et al (Mw in the range of 4500 and 6000 g/mol) [25,28]. For the preparation of the pTHMMAA polymer, 4,4'-azobis(cyanovaleric acid) from Sigma-Aldrich was used as the azo-initiator.…”

Section: Chemicals and Synthesismentioning

confidence: 99%

“…Coated gold colloids has been shown to adsorb from the aqueous solution to polyethylene glycol polymer surfaces in a similar fashion as proteins [48]. We have previously used Lipa-pTHMMAA as a non-fouling layer in immunoassays [26][27]28,29,30].…”

Section: Assembling Of Aunps On the Surface Layersmentioning

confidence: 99%

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Assembly of citrate gold nanoparticles on hydrophilic monolayers

Vikholm-Lundin

Rosqvist

Ihalainen

et al. 2016

Applied Surface Science

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The self-assembled layers were all hydrophilic with Lipa-pTHMMAA exhibiting close to full wetting. AFM reveals that the polyacrylamide layers smoothens the gold surface to a higher extent than the polyethylene glycol and lipoic acid terminated with an amino group. SPR resonance curves were observed to shift to higher angles and become increasingly damped, while also the peaks strongly broaden when large nanoparticles assembled on the surface. Topographical images confirmed that the highest number of particles were assembled on the polyethylene glycol monolayer. By increasing the interaction time more particles could be assembled on the surface.

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Section: Monolayer Formation Of Polyacrylamide and Polyethylene Glycolmentioning

confidence: 91%

Section: Chemicals and Synthesismentioning

confidence: 99%

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Assembly of citrate gold nanoparticles on hydrophilic monolayers

Vikholm-Lundin

Rosqvist

Ihalainen

et al. 2016

Applied Surface Science

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“…Among polymer supports currently available, the use of glycopolymer based on polyacrilamide hydrophobic compounds [44] have played a significant role as multi-dimensional surface matrices. For instance, methyl acrylamide polymers have been used in sandwich assays to detect Human IgG in combination with colloidal gold [45]. The spaced glycopolymer microarray showed enhanced lectin (Arachis hypogaea) binding compared to a non-spaced one [46].…”

Section: D Surfaces: Polymer Supportsmentioning

confidence: 99%

Towards the design of universal immunosurfaces for SPR-based assays: A review

Mauriz

García-Fernández

Lechuga

2016

TrAC Trends in Analytical Chemistry

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Surface biofunctionalization, including chemical activation and attachment of the bioreceptor, is an essential step to provide reliable detection of biomolecular binding events monitored by Surface Plasmon Resonance (SPR), the most employed optical biosensor, and other biosensor techniques. Recent progress in the area of immobilization procedures are aimed at producing reproducible interfacial surfaces that enable the sensitive and specific recognition of the analyte. Antibodies are still the most employed bioreceptors for SPR assays. A wide range of strategies have been proposed to maximize the SPR immunosensor performance by controlling the stability and orientation of the immobilized antibody. This article reviews the most recent advancements in random and oriented antibody immobilization approaches for SPR biosensing applications, with a special focus on the research that have been done to find universal linkers, which can allow the use of the same functionalized surface for different applications.

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“…Nanoparticles as carriers of protein or/and DNA have been studied to amplify detection signals in homogeneous and heterogeneous immunoassays [32][33][34]. However, a polymer brush-modified nanoparticle-based approach has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%