Improved HPLC Method for Determination of Four PPIs, Omeprazole, Pantoprazole, Lansoprazole and Rabeprazole in Human Plasma

Abstract

-PURP OSE:To develop a simple and rapid HPLC method for measuring of four proton-pump inhibitors (PPIs), omeprazole (OPZ), pantoprazole (PPZ), lansoprazole (LPZ) and rabeprazole (RPZ) concentrations in human plasma. METHODS: Following a single step liquid-liquid extraction analytes along with an internal standard (IS) were separated using an isocratic mobile phase of phosphate buffer (10 mM)/acetonitrile (53/47, v/v adjusted pH to 7.3 with triethylamine) at flow rate of 1 mL/min on reverse phase TRACER EXCEL 120 ODS-A column at room temperature. RESULTS: Total analytical run time for selected PPIs was 10 min. The assays exhibited good linearity (r 2 >0.99) over the studied range of 20 to 2500 ng/mL for OPZ, 20 to 4000 ng/mL for PPZ, 20 to 3000 ng/mL for LPZ and 20 to 1500 ng/mL for RPZ. The recovery of method was equal or greater than 80% and lower limit of quantification (LLOQ) was 20 ng/mL for four PPIs. Coefficient of variation and error at all of the intra-day and inter-day assessment were less than 9.2% for all compounds. CONCLUSIONS: The results indicated that this method is a simple, rapid, precise and accurate assay for determination of four PPIs concentrations in human plasma. This validated method is sensitive and reproducible enough to be used in pharmacokinetic studies and also is time-and cost-benefit when selected PPIs are desired to be analyzed.

Improvement and Validation of a High-Performance Liquid Chromatography in Tandem Mass Spectrometry Method for Monitoring of Omeprazole in Plasma

“…The LC-MS/MS the authors used had an LLOQ of 1.5 ng/mL for OME and higher sensitivity and selectivity than authors who used HPLC with UV detection between 3-96 ng/mL [13][14][15][16][17][18][19] . The most relevant LC-MS/MS-based methods have sensitivities of 0.05 ng/mL 23,24 , 0.4 ng/mL 21 , and 1.2 ng/mL 22 , which were slightly lower than those used by the authors of the present study.…”

Pioglitazone HCl Levels and Its Pharmacokinetic Application in Presence of Sucralose in Animals Serum by HPLC Method

“…HPLC/UV is a technique of analysis that could be used for determination of several compounds in plasma or serum [22,23] …”

Simultaneous determination of a binary mixture of pantoprazole sodium and itopride hydrochloride by four spectrophotometric methods

“…Therefore both PAN and ITH are co-formulated together for the treatment of gastric hypersecretory conditions and associated gastrointestinal disorders. Several analytical methods have been developed for the determination of PAN in pharmaceutical preparations such as spectrophotometric methods [3][4][5][6], HPLC methods [7][8][9][10], HPTLC-densitometric methods [11,12] and liquid chromatographic-tandem mass methods (LC-MS/MS) [13,14]. Also several methods have been reported for the determination of ITH in pharmaceutical preparations such as spectrophotometric methods [15][16][17], HPLC methods [18][19][20], HPTLC-densitometric methods [21,22] and spectroflourimetric methods [23].…”