Improved De Novo Draft Genome Sequence of the Nocavionin-Producing Type Strain Nocardia terpenica IFM 0706 and Comparative Genomics with the Closely Related Strain Nocardia terpenica IFM 0406

Abstract

We report an improved de novo draft genome sequence of the human-pathogenic strain Nocardia terpenica IFM 0706T. The resequencing unveiled that the genome size is larger than anticipated, reducing significantly the number of contigs and building a basis for comparison with the closely related strain N. terpenica IFM 0406.

Draft Genome Sequence of the Halophilic Strain Citrobacter braakii AN-PRR1, Isolated from Rhizospheric Soil of Rice (Oryza sativa L.) from Pakistan

“…Genomic DNA (gDNA) of AN-PRR1 was harvested from an overnight culture grown at 37°C in lysogeny broth ( 8 ) on a rotary shaker (220 rpm) and isolated as previously described ( 9 ). The gDNA (20 μg) was sheared using a Covaris g-TUBE device; libraries were constructed using the SMRTbell template preparation v1.0, Sequel binding v2.0, and MagBead v2 kits, followed by size selection using the BluePippin size selection system (Sage Science, Inc.).…”

Genome Sequence of Escherichia coli Stbl4, a Versatile Genetic Tool for Heterologous Expression

“…After 16 h of incubation at 37°C, two single colonies were picked and inoculated into liquid LB at 37°C on a rotary shaker (200 rpm) until saturation. Genomic DNA extraction and PacBio library preparation were conducted as previously described ( 13 ). The 6-kb library was sequenced on a PacBio Sequel instrument using one single-molecule real-time (SMRT) cell.…”