Improved in vitro digestion stability of (−)-epigallocatechin gallate through nanoliposome encapsulation

Zou, Liqiang; Peng, Shengfeng; Liu, Wei; Gan, Lu; Li, Weilin; Liang, Ruihong; Liu, Chengmei; Niu, Jing; Cao, Yan-lin; Liu, Zhen; Chen, Xing

doi:10.1016/j.foodres.2014.07.042

Cited by 128 publications

(92 citation statements)

References 31 publications

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“…Similarly, the PDI only showed a minor change over a 120-day storage period, demonstrating a highly stable catechin nanoemulsion prepared in our experiment. A similar result was found by Huang et al, 40 Zou et al, 36 Ponnuraj et al, 43 and Pace et al, 41 reporting the PDI to be 0.18, 0.29, 0.22, and 0.24, respectively, for catechin and EGCG nanoparticles.…”

supporting

confidence: 82%

“…For instance, Kim et al 6 prepared catechin nanoemulsion composed of cholesterol, phytosterol, glycerol, and water, and the average particle size was determined to be 300 nm by DLS analysis. Similarly, Zou et al 36 prepared EGCG nanoliposome with phospholipid, cholesterol, and Tween 80 as the main components, and the mean particle size was found to be 71.7 nm by DLS analysis. More recently, Gadkari and Balarman 37 prepared catechin nanoemulsion consisting of sunflower oil, Tween 20, Tween 80, lecithin, and water, and the average particle size was shown to be 245 nm by DLS analysis.…”

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confidence: 94%

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Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3

Tsai

Chen

2016

IJN

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Green tea is one of the most commonly consumed natural health beverages in Taiwan’s market, with the major functional component catechin being shown to possess several biological activities such as antioxidation, anticancer, and prevention of cardiovascular disease. The objectives of this study were to develop a high-performance liquid chromatography–mass spectrometry method to determine the variety and content of catechins in green tea leaf waste, a by-product obtained during processing of tea beverage. In addition, catechin nanoemulsion was prepared to study its inhibition effect on prostate cancer cell PC-3. Results showed that a total of eight catechin standards were separated within 25 minutes by using a Gemini C18 column and a gradient mobile phase of 0.1% formic acid (A) and acetonitrile (B) with flow rate at 1 mL/min, column temperature at 30°C, and detection wavelength at 280 nm. Among various extraction solvents, 50% ethanol generated the highest yield of total catechins from tea leaf waste, of which five catechins were identified and quantified. The catechin nanoemulsion was composed of catechin extract, lecithin, Tween 80, and deionized water in an appropriate proportion, with the mean particle size being 11.45 nm, encapsulation efficiency 88.1%, and zeta potential −66.3 mV. A high stability of catechin nanoemulsion was shown over a storage period of 120 days at 4°C. Both catechin extract and nanoemulsion could inhibit growth of PC-3 tumor cells, with the half maximal inhibitory concentration being 15.4 μg/mL and 8.5 μg/mL, respectively. The PC-3 cell cycle was arrested at S phase through elevation of P27 expression and decline of cyclin A, cyclin B, cyclin-dependent kinase 2, and cyclin-dependent kinase 1 expression. In addition, both catechin extract and nanoemulsion could induce apoptosis of PC-3 cells through decrease in B-cell lymphoma 2 (bcl-2) expression and increase in cytochrome c expression for activation of caspase-3, caspase-8, and caspase-9. Taken together, both caspase-dependent and caspase-independent pathways may be involved in apoptosis of PC-3 cells.

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confidence: 82%

mentioning

confidence: 94%

Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3

Tsai

Chen

2016

IJN

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“…The reducing power is an important mechanism in the antioxidant activity of phenolic compounds (Zou, Liu, et al, 2014a). (Mignet et al, 2013;Zou, Peng, et al, 2014b). Like the previous two methods, free algal extract showed lower EC 50 and higher antioxidant activity than loaded nanoliposomes.…”

Section: Determination Of Antioxidant Activitymentioning

confidence: 76%

Manufacturing of nanoliposomal extract from Sargassum boveanum algae and investigating its release behavior and antioxidant activity

Savaghebi

Barzegar

Mozafari

2019

Food Science & Nutrition

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In this paper, the fabrication of algal extract-loaded nanoliposomes was optimized based on the central composite response surface design. Different concentrations of phenolic compounds (500, 1,000, and 1,500 ppm) of algal extract and lecithin (0.5, 1.25, and 2% w/w) were applied for preparation of nanoliposomes at process temperatures of 30, 50, and 70°C. Dependent variables were zeta potential, entrapment efficiency, size, and particle size distribution. The particle size of the loaded nanoliposomes ranged from 86.6 to 118.7 nm and zeta potential from −37.3 to −50.7 mV.The optimal conditions were as follows: 0.5% lecithin, 30°C process temperature, and 1,313 ppm of the phenolic compounds extracted from algae. Under these conditions, the experimental entrapment efficiency of the phenolic compounds was 45.5 ± 1.2%. FTIR analysis has verified the encapsulation of algal extract in nanoliposomes. Algal extract phenolic compounds also increased phase transition temperature (Tc) of nanoliposomes (1.6°C to 6.3°C). Moreover, the thermo-oxidative protection of nanoliposomes for the algal extract has been proved by examining the DSC thermograms.It has been demonstrated that the formulated nanoliposomes have a good stability during storage conditions, and they are able to control the release of phenolic compounds at different pH values. During the encapsulation process, the antioxidant activity of the algal extract has been maintained to an acceptable level. Consequently, algal extract-loaded nanoliposomes can be used as a natural antioxidant in lipidbased foods. K E Y W O R D Salgal extract, antioxidant, encapsulation, nanoliposome, polyphenols 300 |

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“…This liposome encapsulation method has been utilized for epigallocatechin-3-gallate (EGCG) 201 (a polyphenolic compound similar to PGG 85 ) and IC 50 of PGG, which is almost half that of EGCG. 16 This technique can be extended for improvement of PGG bioavailability.…”

Section: Current Issues With Pgg Administrationmentioning

confidence: 99%

Pentagalloyl Glucose and Its Functional Role in Vascular Health: Biomechanics and Drug-Delivery Characteristics

et al. 2018

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Pentagalloyl glucose (PGG) is an elastin-stabilizing polyphenolic compound that has significant biomedical benefits, such as being a free radical sink, an anti-inflammatory agent, anti-diabetic agent, enzymatic resistant properties, etc. This review article focuses on the important benefits of PGG on vascular health, including its role in tissue mechanics, the different modes of pharmacological administration (e.g., oral, intravenous and endovascular route, intraperitoneal route, subcutaneous route, and nanoparticle based delivery and microbubble-based delivery), and its potential therapeutic role in vascular diseases such as abdominal aortic aneurysms (AAA). In particular, the use of PGG for AAA suppression and prevention has been demonstrated to be effective only in the calcium chloride rat AAA model. Therefore, in this critical review we address the challenges that lie ahead for the clinical translation of PGG as an AAA growth suppressor.

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Improved in vitro digestion stability of (−)-epigallocatechin gallate through nanoliposome encapsulation

Cited by 128 publications

References 31 publications

Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3

Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3

Manufacturing of nanoliposomal extract from Sargassum boveanum algae and investigating its release behavior and antioxidant activity

Pentagalloyl Glucose and Its Functional Role in Vascular Health: Biomechanics and Drug-Delivery Characteristics

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