Improved Lycopene Production from Different Substrates by Mated Fermentation of Blakeslea Trispora

Abstract

<jats:p>The production of lycopene from different substrates by Blakeslea trispora in fermentation was investigated. Lycopene productions from 4 and 6% glucose (pH 6.5) in shake flask fermentation were 77.7 and 28.1 mg L−1. Increasing the glucose concentration to 6% resulted in a decrease in lycopene production by 36.2%. A maximum lycopene concentration of 944.8 mg L−1 was detected with 4% glucose supplemented with 1.0 % sunflower oil in fermentor studies. Lycopene productions in the presence of sunflower and corn oils in the fermentor were 12.2 and 11.1 times higher, respectively, then without oil from 4 % glucose in a shake flask. Lycopene production from orange peel was two times higher in the fermentor than in the shake flask. Zygospores of B. trispora are the morphological forms, which are responsible for the production of the lycopene. The highest level of zygospores was correlated with the highest amount of intracellular lycopene in the total biomass dry weight. The media containing only orange powder (1%) gave a 4.9 mg L−1 lycopene production in a fermentor. The biosynthesis of lycopene has been started in most cases simultaneously in the early growth phase even in trace amounts. Maximum lycopene concentration was obtained when the medium was supplied with sunflower and corn oils. There is an indirect relationship between biomass and lycopene concentration.</jats:p>

Enhanced Lycopene Production in Escherichia coli by Expression of Two MEP Pathway Enzymes from Vibrio sp. Dhg

“…The industrial production of carotenoids has conventionally been conducted through an extraction from many natural products, such as tomato and watermelon [11]. However, the extraction method does not meet the growing demand due to limited supply of natural products [12,13]. In addition, its stable production is difficult because of fluctuating lycopene content of natural products: 0.00540-1.50 g lycopene/kg of tomato paste [14], method; however, there are also several critical limitations, including high production cost, low yield, and quality [12,16].…”

“…However, the extraction method does not meet the growing demand due to limited supply of natural products [12,13]. In addition, its stable production is difficult because of fluctuating lycopene content of natural products: 0.00540-1.50 g lycopene/kg of tomato paste [14], method; however, there are also several critical limitations, including high production cost, low yield, and quality [12,16]. To overcome those limitations, microbial production of lycopene has been studied as a promising strategy because it enables stable production through a simple and sustainable process [17,18].…”

Enhanced lycopene Production in <em>Escherichia coli</em> by Expression of Two MEP Pathway Enzymes from <em>Vibrio</em> sp. dhg

“…The industrial production of carotenoids has conventionally been conducted through an extraction from many natural products, such as tomato and watermelon [11]. However, the extraction method does not meet the growing demand due to limited supply of natural products [12,13]. Besides, its stable production is difficult because of fluctuating lycopene content of natural products: 0.00540 -1.50 g lycopene/kg of tomato paste [14], 0.03 -0.07 g lycopene/kg of watermelon [15].…”

“…Besides, its stable production is difficult because of fluctuating lycopene content of natural products: 0.00540 -1.50 g lycopene/kg of tomato paste [14], 0.03 -0.07 g lycopene/kg of watermelon [15]. Chemical synthesis could be considered as an alternative method; however, there are also several critical limitations, including high production cost, low yield, and quality [12,16]. To overcome those limitations, microbial production of lycopene has been studied as a promising strategy, because it enables stable production through a simple and sustainable process [17,18].…”

Bioactive Components in Fermented Foods and Food By-Products

“…Sevgili et al [8] checked the use of different substrates to produce lycopene via Blakeslea trispora fermentation. They confirmed that the medium with natural oil showed more lycopene than the medium that contained only a carbon source.…”