Improved Neuronal Adhesion to the Surface of Electronic Device by Engulfment of Protruding Micro-Nails Fabricated on the Chip Surface

Spira, Micha Ε.; Kamber, Dotan; Dormann, A.; Cohen, Ariel; Bartic, Carmen; Borghs, Gustaaf; Langedijk, Johannes P. M.; Yitzchaik, Shlomo; Shabthai, K.; Shappir, J.

doi:10.1109/sensor.2007.4300363

Cited by 33 publications

(49 citation statements)

References 5 publications

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“…The ultrastructural observations described above revealed that a fraction of the gMμE-MEA were tightly engulfed by cultured myotubes in a similar manner to that described by our laboratory for cultured Aplysia neurons, cardiomyocytes and rat hippocampal neurons2829303132333443. The tight engulfment of the gMμEs by myotubes provides the necessary structural basis to obtain Ohmic access to the myotube sarcoplasm and IN-CELL recording configurations.…”

Section: Resultssupporting

confidence: 63%

“…In this method micrometer-sized, extracellular gold mushroom-shaped microelectrodes (gMμEs) record attenuated synaptic and action potentials (APs) with characteristic features of intracellular recordings while the electrode maintains an extracellular position2829303132333435. These studies show that a range of cell types tightly engulf gMμEs to form high seal resistance ( R s )2930363738.…”

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confidence: 99%

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On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

Rabieh

Ojovan

Shmoel

et al. 2016

Sci Rep

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In contrast to the extensive use of microelectrode array (MEA) technology in electrophysiological studies of cultured neurons and cardiac muscles, the vast field of skeletal muscle research has yet to adopt the technology. Here we demonstrate an empowering MEA technology for high quality, multisite, long-term electrophysiological recordings from cultured skeletal myotubes. Individual rat skeletal myotubes cultured on micrometer sized gold mushroom-shaped microelectrode (gMμE) based MEA tightly engulf the gMμEs, forming a high seal resistance between the myotubes and the gMμEs. As a consequence, spontaneous action potentials generated by the contracting myotubes are recorded as extracellular field potentials with amplitudes of up to 10 mV for over 14 days. Application of a 10 ms, 0.5–0.9 V voltage pulse through the gMμEs electroporated the myotube membrane, and transiently converted the extracellular to intracellular recording mode for 10–30 min. In a fraction of the cultures stable attenuated intracellular recordings were spontaneously produced. In these cases or after electroporation, subthreshold spontaneous potentials were also recorded. The introduction of the gMμE-MEA as a simple-to-use, high-quality electrophysiological tool together with the progress made in the use of cultured human myotubes opens up new venues for basic and clinical skeletal muscle research, preclinical drug screening, and personalized medicine.

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Section: Resultssupporting

confidence: 63%

mentioning

confidence: 99%

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

Rabieh

Ojovan

Shmoel

et al. 2016

Sci Rep

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“…In these studies2324252627282930 we demonstrated that cultured Aplysia neurons tightly engulf the gMμEs to form a high seal resistance. This, together with the increased conductance of the membrane patch that faces the gMμE, makes it possible to record action potentials and subthreshold synaptic potentials with qualities and biophysics similar to those obtained by perforated patch recording electrodes3132.…”

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confidence: 89%

Multisite electrophysiological recordings by self-assembled loose-patch-like junctions between cultured hippocampal neurons and mushroom-shaped microelectrodes

Shmoel

Rabieh

Ojovan

et al. 2016

Sci Rep

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Substrate integrated planar microelectrode arrays is the “gold standard” method for millisecond-resolution, long-term, large-scale, cell-noninvasive electrophysiological recordings from mammalian neuronal networks. Nevertheless, these devices suffer from drawbacks that are solved by spike-detecting, spike-sorting and signal-averaging techniques which rely on estimated parameters that require user supervision to correct errors, merge clusters and remove outliers. Here we show that primary rat hippocampal neurons grown on micrometer sized gold mushroom-shaped microelectrodes (gMμE) functionalized simply by poly-ethylene-imine/laminin undergo self-assembly processes to form loose patch-like hybrid structures. More than 90% of the hybrids formed in this way record monophasic positive action potentials (APs). Of these, 34.5% record APs with amplitudes above 300 μV and up to 5,085 μV. This self-assembled neuron-gMμE configuration improves the recording quality as compared to planar MEA. This study characterizes and analyzes the electrophysiological signaling repertoire generated by the neurons-gMμE configuration, and discusses prospects to further improve the technology.

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“…As demonstrated by Spira et al [12], it is possible to use a biological phenomenon known as ''phagocytosis'' to attach a neuron to a nail-shaped structure by tight engulfment of the nail by the cell membrane. To occur such engulfment requires the biofunctionalization of the sensor surface with phagocytosis-inducing molecules [15].…”

Section: Introductionmentioning

confidence: 99%

Novel concepts for improved communication between nerve cells and silicon electronic devices

Huys

Braeken

Meerbergen

et al. 2008

Solid-State Electronics

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Improved Neuronal Adhesion to the Surface of Electronic Device by Engulfment of Protruding Micro-Nails Fabricated on the Chip Surface

Cited by 33 publications

References 5 publications

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

Multisite electrophysiological recordings by self-assembled loose-patch-like junctions between cultured hippocampal neurons and mushroom-shaped microelectrodes

Novel concepts for improved communication between nerve cells and silicon electronic devices

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