Improved procedure for the isolation of S-adenosylmethionine and S-adenosylethionine

Schlenk, F.; Dainko, J. L.; Stanford, Stephanie M.

doi:10.1016/0003-9861(59)90006-2

Cited by 68 publications

(24 citation statements)

References 6 publications

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“…The precipitate was sedimented by centrifugation, and the deproteinized supernatant was passed through a Dowex-50-H+ column (1.0 cm x 10.0 cm). The column was sequentially eluted with 1 N (100 ml), 2 N (100 ml), and finally 6 N sulfuric acid (50 ml) [12]. The material eluting with 6 N sulfuric acid was collected, pooled, and neutralized by the addition of barium carbonate.…”

Section: Norepinephrinementioning

confidence: 99%

“…The material eluting with 6 N sulfuric acid from the column was neutralized with solid barium carbonate and reduced in volume, in vacua. Unfortunately, this procedure causes degradation of 20-30% of the S-adenosylmethionine [12]; consequently, the estimation of the initial concentration of the compound in the tumor is a minimal value. Attempts to recover S-adenosylmethionine as either the Reinecke salt [3] or phosphotungstic acid precipitate [15] were not successful, possibly because of the large eluate volume containing a small amount of the S-adenosylmethionine (Fig.…”

Section: S-adenosylmethionine In the Tumor Extractmentioning

confidence: 99%

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S-Adenosylmethionine in a Neuroblastoma

Lyon

1971

Pediatr Res

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Section: Norepinephrinementioning

confidence: 99%

Section: S-adenosylmethionine In the Tumor Extractmentioning

confidence: 99%

S-Adenosylmethionine in a Neuroblastoma

Lyon

1971

Pediatr Res

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“…metK84 (Wei & Newman, 2002). SAM was usually prepared from E. coli or yeast extracts, using methods from R. Greene's laboratory which were based on the works of Shapiro and Schlenk (Schlenk et al, 1959;Shapiro & Ehninger, 1966). We have used precisely that method obtained from personal communication with R. Greene.…”

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confidence: 99%

Cell division, one-carbon metabolism and methionine synthesis in a metK-deficient Escherichia coli mutant, and a role for MmuM

2013

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An E. coli K-12 mutant deficient in S-adenosylmethionine (SAM) synthesis, i.e DmetK, but expressing a rickettsial SAM transporter, can grow in glucose minimal medium if provided with both SAM and methionine. It uses the externally provided (R)-enantiomer of SAM as methyl donor to produce most but not all of its methionine, by methylation of homocysteine catalysed by homocysteine methyltransferase (MmuM). The DmetK cells are also altered in growth and are twice as long as those of the parent strain. When starved of SAM, the mutant makes a small proportion of very long cells suggesting a role of SAM and of methylation in the onset of crosswall formation.

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“…The metabolite was isolated from the eluate as the Reineckate [15], and regenerated by the procedure of Schlenck et al [15]. Retaine of aliquots of the solution was further purified by chromatography in the solvent systems I and IV of Eneroth and Lindstedt [16].…”

Section: N6-[14c]methyl-hpteglu3 Was Prepared As Fol-mentioning

confidence: 99%

Mechanism of Methionine Biosynthesis in Rat Liver

Burton

Sakami

1968

European Journal of Biochemistry

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Synthesis of methionine in rat liver by the B,,-independent N5-methyltetrahydropterolyltriglutamate : homocysteine methyltransfcrase reported by Wang, Hoch and Stokstad has been investigated. I n agreement with their results it was found that methionine was formed by crude rat liver supernatant fraction and dialyzed mitochondrial extract on aerobic incubation with N5-methyltetrahydr~pteroyltriglutamate, homocysteine and magnesium chloride. However, the folate derivative could not have been involved in this process since N5-[14C]methyl-tetrahydropteroyltriglutamate did not incorporate I4C into the methionkie. I n the supernatant fraction, methionine was formed by the transmethylation of endogenous betaine with added homocysteine. Rat liver supernatant fraction was found to contain 250 nmoles/ml betaine. In the mitochondrial extract, methionine formation occurred by proteolysis. It is concluded that a B,,-independent N5-rnethyltetrahydropteroyltriglntamate homocysteine methyltransferase does not occur in rat liver.It has been generally considered that methionine is formed in mammalian liver by a vitamin B,2-dependent N6-methyltetrahydrofolate-homocysteine methyltransferase [I -61. Recently, however Wang et al. [7] reported that they had observed the formation of methionine on incubation of methyltetrahydropteroyltriglutamate, homocysteine and magnesium chloride with rat liver supernatant fraction or mitochondrial extract. They interpreted these observations as indication that rat liver also possesses the alternate mechanism of methionine synthesis, catalyzed by the €%,,-independent N5-methyltetrahydropteroyltriglutamate : homocysteine methyltransferase. The validity of the conclusion of Wang et al. [7] has been examined in the study reported in the present communication. ,MATERIALS AND METHODS N6-[14C]Methyl-H,PteGlu3

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Improved procedure for the isolation of S-adenosylmethionine and S-adenosylethionine

Cited by 68 publications

References 6 publications

S-Adenosylmethionine in a Neuroblastoma

S-Adenosylmethionine in a Neuroblastoma

Cell division, one-carbon metabolism and methionine synthesis in a metK-deficient Escherichia coli mutant, and a role for MmuM

Mechanism of Methionine Biosynthesis in Rat Liver

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