Improved Production of Bioactive Glucosylmannosyl-Glycerolipid by Sponge-Associated Microbacterium Species

Lang, Siegmund; Beil, Winfried; Tokuda, Harukumi; Wicke, Corinna; Verena, Lurtz

doi:10.1007/s10126-003-0009-5

Cited by 16 publications

(11 citation statements)

References 12 publications

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Section: Discussionmentioning

confidence: 99%

“…Microbacterium and Bacillus species have been shown to form biofilms and inhabit other marine environments, including sediments, invertebrate tissues (surfaces) and artificial aquacultural ponds (Wicke et al 2000;Lang et al 2004). However, the Bacillus isolate MC6B-22 obtained from a biofilm developed on a copper coupon, to our knowledge is the first report of a tropical intertidal bacterium closely related to B. mojavensis and B. subtilis, organisms considered to be of terrestrial origin.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria

et al. 2007

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Aim: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS‐producing biofilm bacteria. Isolates MC3B‐10 and MC6B‐22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B‐10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B‐10 appears to be a glycoprotein, while EPS MC6B‐22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. EPS MC3B‐10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. The chemical composition of polymer MC6B‐22 suggests its potential biomedical application in tissue regeneration. Significance and Impact of the Study: This is the first report of a Microbacterium species producing EPS with surfactant properties, which expands our knowledge of the micro‐organisms capable of producing these biomolecules. Furthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting EPS‐producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria

et al. 2007

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“…Diversifi ed groups of marine actinobacteria are known to produce different types to anticancer compounds. Several kinds of cytotoxic compounds have been reported from marine actinobacteria [41][42][43][44][45][46][47][48] . The isolated compounds showed signifi cant activity against different cancer cell lines.…”

Section: Biotechnological Importance Of Marine Actinobacteriamentioning

confidence: 99%

Research on marine actinobacteria in India

et al. 2007

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Marine actinobacteriology is one of the major emerging areas of research in tropics. Marine actinobacteria occur on the sediments and in water and also other biomass (mangrove) and substrates (animal). These organisms are gaining importance not only for their taxonomic and ecological perspectives, but also for their unique metabolites and enzymes. Many earlier studies on these organisms were confi ned only to the temperate regions. In tropical environment, investigations on them have gained importance only in the last two decades. So far, from the Indian peninsula, 41 species of actinobacteria belonging to 8 genera have been recorded. The genus, Streptomyces of marine origin has been more frequently recorded. Of 9 maritime states of India, only 4 have been extensively covered for the study of marine actinobacteria. Most of the studies conducted pertain to isolation, identifi cation and maintenance of these organisms in different culture media. Further, attention has been focused on studying their antagonistic properties against different pathogens. Their biotechnological potentials are yet to be fully explored.

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“…Production of glucosylmannosyl-glycerolipid with Microbacterium spec. was reported to be 1.5-fold higher when glycerol instead of glucose is used in media containing the complex medium compounds peptone and yeast extract (Lang et al, 2004, Wicke et al, 2000. When several carbon sources were analyzed for rhamnolipid production by Pseudomonas aeruginosa EM1, glycerol was the best carbon source besides glucose, yielding 4.9 and 7.5 g l -1 respectively (Wu et al, 2008).…”

Section: Biosurfactantsmentioning

confidence: 99%