Improved submerged Aspergillus ficuum phytase production in bench-top bioreactors by optimization of fermentation medium

Coban, Hasan B.; Demirci, Ali

doi:10.1556/066.2015.44.0027

Cited by 8 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, phytase production from A. cuum [33] and fructofuranosidase production from A. niger [13] were enhanced by fungal growth in small pellets. Driouch and co-workers [18] further illustrated that the total biomass obtained when the fungi was growing in large pellets was not signi cantly different to the biomass when fungi was growing in small pellets.…”

Section: Effects Of Glucose Feed Concentration On Do Levels and Mixing As Well As Subsequent Impact On Biomass And Enzyme Productionmentioning

confidence: 82%

“…The experimental data illustrated the importance of bioprocess parameters on fungal morphology and the complex interdependence of the former in controlling the morphology [32]. Therefore, the culture conditions can be controlled to induce fungal growth in pellet morphology and subsequently minimise the viscosity limitations associated with fungal growth in mycelial [13,15,33]. High cell densities are responsible for both increased broth viscosity [13,18] and low DO levels in the culture, due to rapid oxygen uptake [16].…”

Section: Effects Of Glucose Feed Concentration On Do Levels and Mixing As Well As Subsequent Impact On Biomass And Enzyme Productionmentioning

confidence: 95%

See 1 more Smart Citation

Bioprocess Optimisation for High Cell Density Endoinulinase Production from Recombinant Aspergillus niger

Maumela

Rose

Rensburg

et al. 2021

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Endoinulinases gene was expressed in recombinant Aspergillus niger for selective and high-level expression using an exponential fed-batch fermentation. The effects of the growth rate (µ), glucose feed concentration, nitrogen concentration and fungal morphology, on enzyme production were evaluated. A recombinant endoinulinases with a molecular weight of 66 KDa was secreted. Endoinulinases production was growth associated at µ> 0.04 h -1 , which is characteristic of the constitutive gpd promoter used for the enzyme production. The highest volumetric activity (670 U/ml) was achieved at a growth rate of 93% of µ max (0.07 h -1 ), while enzyme activity (506 U/ml) and biomass substrate yield (0.043 g biomassDW /g glucose ) signi cantly decreased at low µ (0.04 h -1 ). Increasing the feed concentration resulted in high biomass concentrations and viscosity, which necessitated high agitation for improved mixing and oxygen. However, the high agitation and low DO levels (ca. 8% of saturation) led to pellet disruption and growth in mycelial morphology. Enzyme production pro les, product (Y p/s ) and biomass (Y x/s ) yield coe cients were not affected by feed concentration and morphological change. The gradual increase in the concentration of nitrogen sources showed that, a nitrogen limited culture was not suitable for endoinulinases production in recombinant A. niger. Moreover, the increase in enzyme volumetric activity was still directly related to an increase in biomass concentration. An increase in nitrogen concentration, from 3.8 to 12 g/L, resulted in volumetric activity increase from 393 to 670 U/ml, but the Y p/s (10053 U/g glucose ) and Y x/s (0.049 g biomasDWs /g glucose ) did not signi cantly change. The data demonstrated the potential of recombinant A. niger and high cell density fermentation for the development of largescale endoinulinases production system.

show abstract

Section: Effects Of Glucose Feed Concentration On Do Levels and Mixing As Well As Subsequent Impact On Biomass And Enzyme Productionmentioning

confidence: 82%

Section: Effects Of Glucose Feed Concentration On Do Levels and Mixing As Well As Subsequent Impact On Biomass And Enzyme Productionmentioning

confidence: 95%

Bioprocess Optimisation for High Cell Density Endoinulinase Production from Recombinant Aspergillus niger

Maumela

Rose

Rensburg

et al. 2021

Appl Biochem Biotechnol

View full text Add to dashboard Cite

show abstract

Microparticle-enhanced Aspergillus ficuum phytase production and evaluation of fungal morphology in submerged fermentation

Coban

Demirci

Turhan

2015

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Phytase can be used in animal's diets to increase the absorption of several divalent ions, amino acids and proteins and to decrease the excessive phosphorus release in manure to prevent negative effects on the environment. This study aimed to enhance the current submerged fungal phytase productions with a novel fermentation technique by evaluating the effect of the various microparticles on Aspergillus ficuum phytase production. It was observed that microparticles prevented bulk fungal pellet growth, decreased average fungal pellet size and significantly increased phytase activity in the submerged fermentation. Microbial structure imaging results showed that the average fungal pellet radius decreased from 800 to 500 and 200 µm by addition of 15 g/L aluminum oxide and talcum, respectively, in shake-flask fermentation. Also, addition of 15 g/L of talcum and aluminum oxide increased phytase activity to 2.01 and 2.93 U/ml, respectively, compared to control (1.02 U/ml) in shake-flask fermentation. Additionally, phytase activity reached 6.49 U/ml within 96 h of fermentation with the addition of 15 g/L of talcum, whereas the maximum phytase activity was only 3.45 U/ml at 120 h of fermentation for the control in the 1-L working volume bioreactors. In conclusion, microparticles significantly increased fungal phytase activity and production yield compared to control fermentation.

show abstract

Enhanced Aspergillus ficuum phytase production in fed-batch and continuous fermentations in the presence of talcum microparticles

Coban

Demirci

Turhan

2015

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

This study aimed to enhance Aspergillus ficuum phytase production in fed-batch and continuous fermentations with addition of talcum microparticles. Phytase activity almost doubled in fed-batch and continuous fermentations by addition of 15 g/l of talcum compared to the control. Effect of talcum on fungal morphology was also shown that addition of talcum provided smaller fungal pellets and more homogenized fermentation broth compared to the control. Average fungal pellet radius decreased from 500 to 100 µm by addition of 15 g/l of talcum in the bioreactors. Also, 15 g/l talcum addition increased phytase productivity and optimum dilution rate in the continuous fermentations from 0.293 to 0.621 U/ml/h and from 0.09 to 0.1/h, respectively, compared to control.

show abstract

Improved submerged Aspergillus ficuum phytase production in bench-top bioreactors by optimization of fermentation medium

Cited by 8 publications

References 15 publications

Bioprocess Optimisation for High Cell Density Endoinulinase Production from Recombinant Aspergillus niger

Bioprocess Optimisation for High Cell Density Endoinulinase Production from Recombinant Aspergillus niger

Microparticle-enhanced Aspergillus ficuum phytase production and evaluation of fungal morphology in submerged fermentation

Enhanced Aspergillus ficuum phytase production in fed-batch and continuous fermentations in the presence of talcum microparticles

Contact Info

Product

Resources

About