Improvement in the production of extracellular lignin peroxidases by Phanerochaete chrysosporium: effect of solid manganese(IV)oxide

Evidence that cellobiose oxidase from Phanerochaete chrysosporium is primarily an Fe(III) reductas

“…Mn02 (autoclaved) at 1 g . 1-I was added after 3 days (Kern, 1989). The pH (not adjusted) was 5.5 at inoculation, but fell during growth to about 4.4.…”

Removal of Aroclor 1254 by the white rot fungus Coriolus versicolor in the presence of different concentrations of Mn(IV)oxide

“…Therefore, it is possible that manganese also regulates the overall mineralisation of target compounds like persistent recalcitrant chemicals and in this case, Aroclor (Bonnarme and Jeffries, 1990). Perez and Jeffries (1992) and Kern (1989Kern ( , 1990, also found that Mn(II) induced MnP and that simultaneous repression of LiP was due to the presence of soluble complexes of simple organic acids. The higher concentrations of Mn ions, provided by solid Mn0 2 , indicated a higher production of MnP in our study for all trials, excepting the culture containing 57.5 mM Mn0 2 .…”

“…The addition of high concentrations of Mn0 2 to cultures of C. versicolor had the ability to improve production, enzymatic activity and stability of the manganese(II)-peroxidase enzyme produced by this fungus (Kern, 1989). The results showed that there existed a relationship between Mn0 2 concentration and the production of manganese(II)-peroxidase and H 2 0 2 production.…”

“…Also shown was a positive relationship between the concentration of Mn0 2 , added on day 4 of incubation and the r a t e a nd e x t e nt o f A ro c l o r d e g ra d a t i o n . P re v i o u s s t u d i e s ha v e s ho w n t ha t t he a d d i t i o n o f M n0 2 t o c u l t u re s o f P. chrysosporium had the ability to improve production, enzymatic activity,and stability of the lignin peroxidase enzyme produced by this fungus (Bonnarme and Jeffries, 1990;Kern, 1989Kern, , 1990. The effect of Mn0 2 on the production and activity of Mn-dependent peroxidase has not yet been determined.…”

In vivo decolourization of the polymeric dye poly R‐478 by corncob cultures of Phanerochaete chrysosporium

“…Besides lignin, P. chrysosporium is also capable of oxidizing various recalcitrant xenobiotics released to the environment by human activity [2], including several synthetic dyes [3]. Extracellular peroxidases secreted by this fungus play an important role in the degradation of such compounds and these may be activated by adding certain compounds, such as veratryl alcohol [4] and manganese (IV) oxide [5] to the culture medium. Most studies on lignin biodegradation have been performed in liquid cultures, which do not reflect the natural living conditions (wood) of these fungi.…”