Improvement of Brazilian bioethanol production – Challenges and perspectives on the identification and genetic modification of new strains of Saccharomyces cerevisiae yeasts isolated during ethanol process

Souza, Jonas Paulino de; Prado, Cleiton Dias do; Eleuthério, Elis C. A.; Bonatto, Diego; Malavazi, Iran; Cunha, Anderson Ferreira da

doi:10.1016/j.funbio.2017.12.006

Cited by 43 publications

(20 citation statements)

References 66 publications

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“…Sci. 2019, 9, 254 5 of 13 bioethanol market, the so-called ProAlcool [34]. The program encouraged the production of ethanol from sugar cane and established a law that required blending petrol with ethanol at the ratio of 80:20.…”

Section: Biofuels In the European Union The Usa And Brazilmentioning

confidence: 99%

Selected Aspects of Biofuels Market and the Electromobility Development in Poland: Current Trends and Forecasting Changes

et al. 2019

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This work presents basic information associated with markets of selected alternative fuels used in transport, such as methyl esters, conventional bioethanol and lignocellulosic bioethanol, and the market of electrical vehicles. Legal conditions, which stimulate development and regulate the mode of functioning of the liquid biofuel market until 2020 are discussed, based on provisions of EU directives. Data on biofuel production in Poland are presented, as well as biofuel consumption in the EU, the USA and Brazil in 2017. The most important conclusions of the proposal for a directive on the promotion of renewable energy sources in transport in EU member states in years 2021–2030 are discussed. The authors have also indicated the key legal and territorial conditions associated with the development of electromobility and present basic information on electric vehicles in Poland and Europe. The results of the research on the attractiveness of these sectors in 2018 are presented and compared with the results obtained in years 2007–2017. A score-based sector attractiveness method was used in the research.

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Section: Biofuels In the European Union The Usa And Brazilmentioning

confidence: 99%

Selected Aspects of Biofuels Market and the Electromobility Development in Poland: Current Trends and Forecasting Changes

et al. 2019

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“…Propolis was collected from beehives located in Egypt and stored at -20 o C. The ethanolic extract was prepared as described by (Paulino de Souza et al, 2018). Briefly, propolis samples were frozen at -24°C and a grinder was used to break them into small pieces.…”

Section: Preparation Ethanol Propolis Extractmentioning

confidence: 99%

The Effects of Green Tea and Propolis Extracts on pro-inflammatory cytokines TNF-?, IFN-?, IL2, and Immunoglobulin Production in Experimentally Infected Rabbits with Bovine Herpesvirus-1.

Zeedan¹,

El-Razik²,

Abdel-Shafy³

et al. 2019

jwpr

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Bovine herpesvirus 1 (BHV-1) is a highly contagious viral pathogen which causes infectious bovine rhinotracheitis in bovine worldwide. Currently, there is no antiviral prophylactic treatment available capable of the complete cure of the viral disease and facilitating recovery from latent infection in animals. The present study aimed to evaluate antiviral activities of Water Green Tea Extract (WGE) and Ethanol Propolis Extract (EPE) against BHV-1 virus comparing to commercial Acyclovir (ACV) in vitro in Madin-Darby Bovine Kidney (MDBK) cell line and in vivo in rabbits as a laboratory animal's model. The cytotoxicity assay was determined the safe dose of water green tea, and Ethanol propolis extracts and evaluated antiviral activity of each extract on infected MDBK with BHV-1. The fifteen rabbits were divided accidentally into five groups. Groups 1, 2 and 3 were inoculated with BHV-1 virus 10 7 TCID50/250 ul in nostrils and received propolis ethanol, water green tea extracts and ACV antiviral for 7 dpi respectively. Group 4 was inoculated with BHV-1 virus 107 TCID50/250 ul in nostrils without extracts or commercial drug. Group 5 was considered as control negative. Results of in-vitro study showed water green tea, and ethanol propolis extracts were potent inhibitor on BHV-1, which showed 80% protection against this virus and dropped in viral titer more than ACV. In vivo study of treated infected animals with WGE, EPE and ACV reduced clinical signs, elevated cytokines, and antibody production levels and failed re-isolated or detect DNA in blood or nasal samples swabs. Non treaded infected rabbits group developed respiratory clinical signs, humoral response and re-isolated BHV-1 and detected viral DNA of BHV-1 in blood, and nasal swabs from experimentally infected rabbits. In conclusion, propolis and green tea extracts were able to prevent virus replication and reduced CPE in MDBK cell cultures infected with BHV-1 and able to induce cytokines and antibodies levels production.

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“…The fermentation occurs between 28-35 ºC and, during the summer, the maintenance of this temperature is only possible through water cooler use installed in fermentation tanks. This process is crucial to maintain the temperature within an ideal range, not exceeding 35 ºC, but is costly and requires large quantities of water [5].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to temperature, several other stressing conditions such as pH variation, osmotic stress, contamination by bacteria, and wild yeasts can affect the fermentative process [5]. Therefore, the production process should be performed using strains able to survive all of these conditions in the fermentation tank.…”

Section: Introductionmentioning

confidence: 99%

“…Although these strains have had a high performance for several years, the constitution regarding Brazilian fermentation substrates has been changing throughout the last years, especially after the sugar cane burn was prohibited by law. As a result, there was a lower persistence of these strains in the fermentation tanks during the whole harvest that were then replaced by wild yeasts in several mills in Brazil [5]. Otherwise CAT-1 and PE-2 were not able to fermentate appropriately above 35 ºC, reducing their viability [10] Aiming to maintain productivity or to improve the Brazilian fermentation process, our research group has been isolating new strains from distilleries since 2009 to identify new Saccharomyces cerevisiae strains that can adapt to the new conditions or that have new characteristics as ethanol, sugar and thermotolerance [5].…”

Section: Introductionmentioning

confidence: 99%

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Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production and its application in high temperature fermentation

Prado

Mandrujano

Souza

et al. 2020

Preprint

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Abstract Background The use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This increment in temperature could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF) of starch or lignocellulosic biomass. Results We identified and characterized the physiology of a new thermotolerant strain able to fermentate at 40 °C while producing high yields of ethanol. Our results showed that, in comparison to the industrial yeast CAT-1, our strain was more resistant to various stressors generated during the production of first- and second-generation ethanol, and it also was able to change the pattern of genes involved in sucrose assimilation (SUC2 and AGT1). The formation of secondary products of fermentation was different at 40ºC, with reduced expression of genes involved in the formation of glycerol (GPD2), acetate (ALD6 and ALD4), and acetyl-CoA (ACS2). Conclusion The LBGA-01 strain is a thermotolerant strain that modulates the production of key genes, changing metabolic pathways during high-temperature fermentation, and increasing its tolerance to the high concentration of ethanol, sugar, acetic lactic, acetic acid, furfural and HMF. This indicates that this strain can be used to improve first- and second-generation ethanol production in Brazil.

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Improvement of Brazilian bioethanol production – Challenges and perspectives on the identification and genetic modification of new strains of Saccharomyces cerevisiae yeasts isolated during ethanol process

Cited by 43 publications

References 66 publications

Selected Aspects of Biofuels Market and the Electromobility Development in Poland: Current Trends and Forecasting Changes

Selected Aspects of Biofuels Market and the Electromobility Development in Poland: Current Trends and Forecasting Changes

The Effects of Green Tea and Propolis Extracts on pro-inflammatory cytokines TNF-?, IFN-?, IL2, and Immunoglobulin Production in Experimentally Infected Rabbits with Bovine Herpesvirus-1.

Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production and its application in high temperature fermentation

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