Improvement of enzymatic performance of Asclepias curassavica L. proteases by immobilization. Application to the synthesis of an antihypertensive peptide

Origone, Anabella Lucía; Barberis, S; Illanes, Andrés; Guzmán, Fanny; Camí, Gerardo Enrique; Liggieri, Constanza Silvina; Martínez, Ronny; Bernal, Claudia

doi:10.1016/j.procbio.2020.05.013

Cited by 4 publications

(5 citation statements)

References 37 publications

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“…• S. melongena (ZSM‐5 zeolite) [303] • A. curassavica protease (octyl‐glyoxyl‐silica) [304] • Antiacanthain (glyoxyl‐silica) [305] • Granulosain f (glyoxyl‐silica) [305] • Papain (mesoporous silica) [306] …”

Section: Biotechnological Applications Of Plant Proteases: Perspectiv...mentioning

confidence: 99%

Production of Plant Proteases and New Biotechnological Applications: An Updated Review

2022

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An updated review of emerging plant proteases with potential biotechnological application is presented. Plant proteases show comparable or even greater performance than animal or microbial proteases for by‐product valorization through hydrolysis for, for example, cheese whey, bird feathers, collagen, keratinous materials, gelatin, fish protein, and soy protein. Active biopeptides can be obtained as high added value products, which have shown numerous beneficial effects on human health. Plant proteases can also be used for wastewater treatment. The production of new plant proteases is encouraged for the following advantages: low cost of isolation using simple procedures, remarkable stability over a wide range of operating conditions (temperature, pH, salinity, and organic solvents), substantial affinity to a broad variety of substrates, and possibility of immobilization. Vegetable proteases have enormous application potential for the valorization of industrial waste and its conversion into products with high added value through low‐cost processes.

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Section: Biotechnological Applications Of Plant Proteases: Perspectiv...mentioning

confidence: 99%

Production of Plant Proteases and New Biotechnological Applications: An Updated Review

2022

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Section: Introductionmentioning

confidence: 99%

“…Synthesis of peptides will not proceed efficiently in aqueous medium where the hydrolytic potential of the enzyme prevails. The design of reaction media is a major challenge for peptide synthesis, since proteases, different from lipases, are not structurally conditioned to act in such environments ( Illanes et al, 2009 ; Origone et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, enzymatic processes are usually cheaper and more sustainable than chemical processes (Barberis et al, 2018). However, only some plant proteases have been used in peptide synthesis processes (Origone et al, 2018(Origone et al, , 2020Adaro et al, 2021). Vallés et al (2004) reported the isolation, partial purification, and characterization of a proteolytic extract obtained by grinding the ripe fruits of Solanum granuloso leprosum (Solanaceae), a South American native plant.…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic synthesis of new antimicrobial peptides for food purposes

et al. 2023

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Growing consumer awareness of the potential negative health effects of synthetic antibiotics has prompted the search for more natural preservatives that can improve the safety and quality of food. In this study we report the enzymatic synthesis of N-α-[Carbobenzyloxy]-Ile-Gln (Z-IQ) which is the precursor of Ile-Gln (IQ), a new antibacterial dipeptide, using an aqueous–organic biphasic system formed by 50% (v/v) ethyl acetate in 0.1 M Tris – HCl buffer pH 8. A partially purified proteolytic extract from the fruits of Solanum granuloso leprosum, named granulosain, proved to be a robust biocatalyst for the synthesis of Z-IQ, eliciting 71 ± 0.10% maximal peptide yield in the above described conditions. After cleaving and purifying IQ dipeptide, antimicrobial activity was assayed against Staphylococcus aureus ATCC 25923, Staphylococcus hominis A17771, and Staphylococcus aureus C00195, and MIC values between 118 ± 0.01 μg/mL and 133.7 ± 0.05 μg/mL were obtained. In addition, IQ showed MIC of 82.4 ± 0.01 μg/mL and 85.0 ± 0.00 μg/mL against Escherichia coli ATCC 25922 and Escherichia coli A17683, respectively. IQ did not show inhibitory activity against single-drug resistance (SDR) strains, such as Klebsiella oxytoca A19438 (SDR) and Pseudomonas aeruginosa C00213 (SDR), and against multidrug-resistant Enterococcus faecalis I00125 (MDR). IQ also caused growth inhibition of Helicobacter pylori NCTC 11638 and three wild-type H. pylori strains, which are sensitive to AML, MTZ, LEV and CLA (H. pylori 659), resistant to LEV (H. pylori 661 SDR), and resistant to MTZ (H. pylori 662 SDR). Finally, this study contributes with a new dipeptide (IQ) that can be used as an antimicrobial agent for food preservation or as a safe ingredient of functional foods.

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“…This proteinous metabolite functions as a biocatalyst for chemical reactions in living things, with protease found to be primarily utilized (6)(7)(8). As a biocatalyst, protease is found to hydrolyze peptide bonds from proteins, with the number of compounds produced depending on the enzymeproducing bacteria (9). This enzyme is primarily vital for industrial purposes, including the food, pharmaceutical, cosmetic, and feed probiotic industries (10,11).…”

Section: Introductionmentioning

confidence: 99%

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

Fitriadi¹,

palupi²,

Setyawan³

et al. 2023

IJVS

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Vannamei shrimp culture in intensive and traditional ponds is developing rapidly in Indonesia. The development starts with technology, high density, and feeding systems. It results in very high organic material produced in the aquaculture ponds, and this water quality management is necessarily maintained. This study aims to isolate and identify the molecular of proteolytic bacteria from Vannamei shrimp aquaculture ponds using a different system that is as probiotic agents. This study used a survey method and random sampling technique from traditional and intensive shrimp aquaculture. The results showed that the proportion of proteolytic bacteria in traditional ponds was higher than that in intensive ponds. The screening based on colony morphology and highest activity index obtained 4 bacterial isolates of Vibrio sp. and Pseudoalteromonas white an identity value of 98.23-99.80%. The value of this activity was observed at 7.0 cm, subsequently inhibiting Vibrio sp. by 1.5 cm. The bacteria were also found to develop at pH 7 and a temperature of 28°C adequately. Additionally, the maximum proteolytic activity at a 48-hours incubation indicated their potential relevance as a biotechnological probiotic. Further studies on the potential of Pseudoalteromonas ganghwensis strains found that the bacteria have antimicrobial activity and are sensitive to antibiotics that can be used as aquaculture probiotic agents.

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Improvement of enzymatic performance of Asclepias curassavica L. proteases by immobilization. Application to the synthesis of an antihypertensive peptide

Cited by 4 publications

References 37 publications

Production of Plant Proteases and New Biotechnological Applications: An Updated Review

Production of Plant Proteases and New Biotechnological Applications: An Updated Review

Enzymatic synthesis of new antimicrobial peptides for food purposes

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

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