Improvement of Hyperglycemia and Sexual Dysfunction in Diabetic Female Rats by an Artificial Endocrine Pancreas Developed from Mouse .BETA. Cells

Abstract

Abstract:We investigated the effects of a bioartificial endocrine pancreas (Bio-AEP) produced by mouse b cells on sexual dysfunction of streptozotocin (STZ)-induced diabetic female rats. Female rats were administered STZ (60 mg/kg BW, i.v.) at the age of 10 weeks and transplanted with a Bio-AEP including mouse b cells at the age of 14 weeks (STZ+Bio-AEP group). Lordosis and proceptive sexual behavior of female rats were observed. The results showed that after the Bio-AEP transplant blood glucose recovered from 380-450 mg/dl induced by streptozotocin to 140-230 mg/dl and suppressed lordosis and proceptive behavior also recovered. These results suggest that it is possible to reverse sexual dysfunction by continuous administration of mouse insulin. Key words: bioartificial endocrine pancreas, diabetic rats, streptozotocin Japan Diabetes is one of the causes of sexual dysfunction, inducing neuroendocrine dysfunction [11,13,19,21] and apoplexy [2] based on hyperglycemia and insulin insufficiency. Rats are usually used as a diabetic animal models as diabetes can be induced by streptozotocin (STZ) for observations of sexual behavior [18,21,22,23]. Both men and women can contract type 1 and 2 diabetes. Especially, diabetes during pregnancy is common in primiparous women and it is significantly associated with a number of risks of pregnancy and birth, including hypertension [20], intra-amniotic infection [1], and an increased rate of Caesarean section [1,12]. In addition, the children born to mothers with diabetes in pregnancy are more likely to be premature [12] and develop metabolic abnormalities in later life [17]. Therefore, we focused on sexual behavior testing in females in this study. Ohgawara et al. (1995) created a diffusion chamber as a bioartificial endocrine pancreas (Bio-AEP) with b cells for xenotransplantation [15]. The b cells in the Bio-AEP were maintained for a long time because Bio-AEP included b cells with an agarose-PVMA-collagen matrix [15]. Ohgawara et al. (1996) reported that the Bio-AEP suppressed hyperglycemia in STZ-induced diabetic rats [16]. We became interested in these reports on the Bio-AEP, since we thought it might improve -Note-

Diabetes during pregnancy enhanced neuronal death in the hippocampus of rat offspring

“…a Diabetic (STZ-D) group (n = 11) b Diabetic treated with insulin (STZ-INS) group (n = 11) c Controls (CON, n = 8) These allocations were based on difference of fertility between diabetic and non-diabetic rats. Existing evidence suggest direct deleterious effects of hyperglycemia on female reproductive functions (Bestetti et al, 1985;Ballester et al, 2007;Hashimoto et al, 2010). Thus, we considered 11 animals for each diabetic group to achieve acceptable number of pregnant females per group.…”