Improvement of Selectivity and Stability of Amperometric Detection of Hydrogen Peroxide Using Prussian Blue‐PAMAM Supramolecular Complex Membrane as a Catalytic Layer

Abstract

A novel hydrogen peroxide (H 2 O 2 ) sensor was fabricated by using a submonolayer of 3-mercaptopropionic acid (3-MPA) adsorbed on a polycrystalline gold electrode further reacted with poly(amidoamine) (PAMAM) dendrimer (generation 4.0) to obtain a film on which Prussian Blue (PB) was later coordinated to afford a mixed and stable electrocatalytic layer for H 2 O 2 reduction. On the basis of the electrochemical behaviors, atomic force microscopy (AFM) and X-ray photoelectron spectra (XPS), it is suggested that the PB molecules are located within the dendritic structure of the surface attached PAMAM dendrimers. It was found that the PB/PAMAM/3-MPA/Au modified electrode showed an excellent electrocatalytic activity for H 2 O 2 reduction. The effects of applied potential and pH of solution upon the response of the modified electrode were investigated for an optimum analytical performance. Even in the presence of dissolved oxygen, the sensor exhibited highly sensitive and rapid response to H 2 O 2 . The steady-state cathodic current responses of the modified electrode obtained at À 0.20 V (vs. SCE) in air-saturated 0.1 mol L À1 phosphate buffer solution (PBS, pH 6.50) showed a linear relationship to H 2 O 2 concentration ranging from 1.2 Â 10 À6 mol L À1 to 6.5 Â 10 À4 mol L À1 with a detection limit of 3.1 Â 10 À7 mol L À1. Performance of the electrode was evaluated with respected to possible interferences such as ascorbic acid and uric acid etc. The selectivity, stability, and reproducibility of the modified electrode were satisfactory.

Electrocatalytic oxidation and reduction of H2O2 on vertically aligned Co3O4 nanowalls electrode: Toward H2O2 detection

“…However, electrochemical methods have emerged as preferable, owing to their relatively low cost, efficiency, high sensitivity, and ease of operation [35][36][37]. Different materials, such as noble metals, macrocycle complex of transition metals, carbon nanotubes, and enzymes have been used to modify electrodes for the reduction/oxidation as well as the detection of H 2 O 2 [1,[37][38][39][40][41][42][43][44][45][46]. In recent years, new efforts have been emphasized on the use of novel metal oxides nanomaterials to modify electrode surfaces for enhanced oxidation/reduction and sensitive detection of H 2 O 2 because metal oxide nanomaterials are easy to be synthesized, have very high surface to volume ratio, and show great potential to enhance electrocatalytic activity and promote electron-transfer reactions at a lower overpotential [1,11,47,48].…”

Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide

“…Many electrochemical [11][12][13][14][15][16] spectrophotometry 17,18 have been reported for the determination of hydrogen peroxide. Electrochemical sensors are suitable since they achieve low detection limits and rapid response time.…”

Enhancement of the Electrochemical Performance of Prussian Blue Modified Electrode via Ionic Liquid Treatment

“…2A and ). The greater loading of electroactive species on IL modified electrode was promising to enhance the analytical performance of PB film [14].…”