Improvement of the rapid immunoperoxidase staining method for intraoperative pathological diagnosis of pancreatic cancer using microwave irradiation

Ichihara, Toshiaki; Nakao, Akimasa; Suzuki, Yuichi; Sakamoto, Junichi; Nonami, Toshiaki; Harada, Akio; Nishimura, Hiroshi

doi:10.1002/jso.2930420316

Cited by 21 publications

(26 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…have been proposed that use microwave [7], high quality reagents [8] [9] [10] [11] [12], intermittent microwave [13], and ultrasound [14]. In these studies, only a few papers were focused on the application of these techniques for central nervous system (CNS) tumors [10] [11].…”

Section: In the Last Three Decades Several Methods For Rapid Immunohmentioning

confidence: 99%

Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

et al. 2014

View full text Add to dashboard Cite

Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current (AC) electric field to facilitate the antigen-antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 91.8% (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma.To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors. (192 words)

show abstract

Section: In the Last Three Decades Several Methods For Rapid Immunohmentioning

confidence: 99%

Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Several studies have tried to reduce the time for immunostaining using various different techniques (21,24,29). However, some of the methods described still require 20 min or more to perform (45,46).…”

Section: Discussionmentioning

confidence: 99%

“…However, directly labelled primary antibodies are usually more expensive and are available only for a limited range of antigens. Using microwave treatment and an ABC method, Ichihara et al (24) were able to reduce the time for immunostaining frozen sections to 13 min. The most rapid IHC method applied on Table III.…”

Section: Discussionmentioning

confidence: 99%

“…So far, however, standard IHC was not readily applicable to the intraoperative examination because it required too much time (4-6 h). However, according to techniques developed during the last few years, the intraoperative immunohistochemical examination of frozen sections has become possible allowing for a histopathological diagnosis to be conducted within 10-15 min (21)(22)(23)(24)(25)(26)(27)(28)(29)(30). However, most of the methods described exhibited certain disadvantages, such as a time-consuming procedure (≥20 min) or the requirement of directly labelled primary antibodies.…”

Section: Introductionmentioning

confidence: 99%

“…However, most of the methods described exhibited certain disadvantages, such as a time-consuming procedure (≥20 min) or the requirement of directly labelled primary antibodies. However, by applying microwave treatment and an HRP procedure, Ichihara et al (24) were able to immunostain frozen sections intraoperatively within 13 min. Recently, the immunohistochemical detection systems EnVision™ and Histofine ® have become commercially available, and the EnVision system has been described as a very sensitive and rapid detection system in IHC (31,32).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid immunohistochemical detection of tumor cells in gastric carcinoma

Mönig

Luebke²,

Soheili³

et al. 2006

Oncol Rep

View full text Add to dashboard Cite

Abstract. The detection of single tumor cells or tumor cell clusters represents an important issue in intraoperative frozen section analysis. For example, surgical margins may be evaluated in order to minimize the number of additional operations. Furthermore, intraoperative diagnosis of lymph node micrometastasis (LNM) may help to define the area of appropriate lymph node dissection. In addition to haematoxylin and eosin (H&E)-stained sections, immunohistochemical detection of single tumor cells or cell clusters may be helpful in this context. The aim of this study was to evaluate the clinical significance, reliability and sensitivity of intraoperative rapid immunostaining of frozen sections. Therefore, we compared the results of rapid immunohistochemical staining of frozen sections and paraffin sections applying the EnVision™ and Histofine ® detection systems. In a prospective immunohistochemical study, paraffin and frozen sections of 20 gastric cancer specimens were analyzed. Paraffin as well as frozen sections were stained immunohistochemically applying the EnVision and Histofine detection systems. As primary antibodies, AE1/AE3 (anti-cytokeratin), EMA (anti-MUC1) and B lymphocyte marker anti-CD20 were applied. The rapid immunostaining procedure was able to be completed within 10-13 min. Rapid immunohistochemical staining of frozen and paraffin sections of the same tumors resulted in comparable immunoreactivity. The rapid EnVision and Histofine procedures allowed immunostaining of frozen sections in less than 13 min. These methods can represent useful additional tools in routine surgical pathology and research, enabling a more accurate frozen section diagnosis compared to staining with H&E alone. Intraoperative rapid immunostaining can be a simple and useful technique to detect LNM.

show abstract

Clinical usefulness of CA-19-9 in pancreatic carcinoma

Nakao

Oshima

Nomoto

et al. 1998

Semin. Surg. Oncol.

View full text Add to dashboard Cite

Preoperative and postoperative serum levels of carbohydrate antigen‐(CA)19‐9 in 148 patients with carcinoma of the pancreas were studied. All 18 patients with carcinoma of the pancreas of Stage I, II, and III were resectable, and their pre‐operative serum levels of CA‐19‐9 were under 1,344 U/ml. Pre‐operative serum levels of CA‐19‐9 in patients with Stage IV ranged widely between 5 and 32,240 U/ml. The postoperative survival rate was significantly superior in patients (n = 15) whose CA‐19‐9 pre‐operative serum levels were less than 2,000 U/ml compare to those (n = 64) whose levels were over that level. Fifteen resectable patients who showed serum levels of CA‐19‐9 over 2,000 U/ml pre‐operatively died within 2 years postoperatively due to recurrence, especially by liver metastasis in spite of aggressive surgery. Intraoperative quick immunostaining of CA‐19‐9 and carcinoembryonic antigen (CEA) was useful to diagnose intrapancreatic carcinoma development on frozen sections of cut margin of the pancreas and also useful in abdominal washing cytology combined with conventional staining. Semin. Surg. Oncol. 15:15–22, 1998. © 1998 Wiley‐Liss, Inc.

show abstract

Improvement of the rapid immunoperoxidase staining method for intraoperative pathological diagnosis of pancreatic cancer using microwave irradiation

Cited by 21 publications

References 13 publications

Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

Rapid immunohistochemical detection of tumor cells in gastric carcinoma

Clinical usefulness of CA-19-9 in pancreatic carcinoma

Contact Info

Product

Resources

About