Improvements of enzyme activity and enantioselectivity in lipase-catalyzed alcoholysis of (R,S)-azolides

Towards a green enantiomeric esterification of R/S-ketoprofen: A theoretical and experimental investigation

“…Pharmaceutical industries apply the enantioselectivity of lipases for preparing different pharmaceuticals and fine chemicals containing a chiral center [1,7]. Several drugs are found in their racemic form.…”

Enzyme catalytic promiscuity: asymmetric aldol addition reaction catalyzed by a novel thermophilic esterase in organic solvent

“…It is well known that a proper selection of organic solvent is essential in biocatalytic reactions because the activity of enzymes may be affected by the polarity of organic media (26). The polarity of the organic solvents can be quantitatively measured by the value of log P, the logarithm of the partition coefficient of a given solvent between water and 1-octanol.…”

Immobilization of lipase onto cellulose ultrafine fiber membrane for oil hydrolysis in high performance bioreactor

“…Lipases are hydrolytic enzymes capable of catalyzing a wide range of reactions, such as alcoholysis, hydrolysis, trans-esterifications, aminolysis and enantiomer resolution, therefore having been widely applied in the food, pharmaceutical, and detergent industries (Compton et al 2006;Deng et al 2005;Garcia-Urdiales et al 2009;Huang et al 2008;Ragupathy et al 2010;Wu et al 2010). In practical applications, like most enzymes, lipases are often attached or incorporated onto or into inert, insoluble supports, which can offer better catalytic stability, the potential for continuous operations, feasible catalyst recycling, significant reduction in operation costs, and simplified product purification process, thus overcoming the thermal stability, reusability, and recoverability limitations associated with the free enzymes (Noureddini et al 2005;Huang et al 2006).…”