In Ovo and Day of Hatch Application of a Live Infectious Bursal Disease Virus Vaccine to Commercial Broilers

Ashash, Udi; Noach, Caroline; Perelman, B.; Costello, Charles G.; Sansalone, Pablo; Brazil, Thiago; Raviv, Ziv

doi:10.1637/aviandiseases-d-19-00087

Cited by 11 publications

(27 citation statements)

References 27 publications

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“…This is a common phenomenon and adheres to both the inactivated and live vaccines, also including vector vaccines. When birds are highly MDA+ with homologous antibodies to the antigen the birds are vaccinated against, a decreased level of maternal antibodies results from the vaccination because they are used to neutralise the antigen, as also do a lower level of humoral immunity and consequently, a more severe clinical course of the disease after experimental infection than in MDA− birds at the moment of vaccination (1,7,8,17).…”

Section: Discussionmentioning

confidence: 99%

IFNγ production profile in turkeys of different immunological status after TRT vaccination

Smiałek

Tykałowski

Dziewulska

et al. 2020

Journal of Veterinary Research

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AbstractIntroductionDespite vaccination against avian metapneumoviruses (aMPV), cases of turkey rhinotracheitis (TRT) caused by aMPV field strains are frequently reported. Differences have been shown in the level of immune system stimulation after aMPV vaccination between turkeys that do and do not possess specific anti-aMPV maternally derived antibodies (MDA). The article describes the influence of MDA on the production of IFNγ in the spleen of aMPV-vaccinated turkeys.Material and MethodsMDA+ or MDA− turkeys were vaccinated against TRT after hatching or on the 14th day of life. Spleen samples were collected 3, 7, and 14 days post vaccination for mononuclear cell isolation. Real-time PCR, flow cytometry, and the enzyme-linked immunospot assay were used to evaluate the levels of IFNγ gene expression, production, and secretion by cells within the spleen samples.ResultsIncreased IFNγ gene expression was noticed after vaccination only in birds that did not possess MDA or possessed MDA at relatively low level (MDA+ birds vaccinated at 14 DOL). In all birds, an increased percentage of T lymphocytes producing IFNγ was recorded. The proportion of anti-aMPV IFNγ-secreting cells was increased only in MDA− birds.ConclusionBesides having a protective role, MDA are known to interfere with vaccination efficacy. The analysis of our results confirms that MDA can decrease the level of immune system stimulation after aMPV vaccination of turkeys.

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Section: Discussionmentioning

confidence: 99%

IFNγ production profile in turkeys of different immunological status after TRT vaccination

Smiałek

Tykałowski

Dziewulska

et al. 2020

Journal of Veterinary Research

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“…In an effort to improve the protective characteristics of IBDV hatchery vaccination, we have evaluated the prevailing dogma concerning conventional live IBDV vaccination of in-ovo and posthatch maternally immunized chicks with the MB-1 live vaccine strain. Our previous field studies on MB-1 in Latin America, Africa, and Israel have been shown to be safe and effective in commercial broiler chickens having varying MDA ( Ashash et al, 2019 ). Here in, we have proposed to evaluate the feasibility of DOH application of MB-1 to commercial broiler chickens in India.…”

Section: Discussionmentioning

confidence: 99%

“…Bursal tissues were fixed in 10% neutral buffered formalin and paraffin embedding procedures, and the sections were stained with hematoxylin and eosin (H&E). The bursas of the 4 trials were evaluated microscopically by the same certified veterinary pathologist and scored in the range of 0 for normal bursa to 5 for severely affected bursa in accordance with the European Pharmacopeia bursal lesion score scale ( Ashash et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…Initially, all RNA samples were investigated by the real-time RT-PCR for the presence of IBDV RNA, using primers and probe for the VP3 gene in the A segment of the IBDV genome ( Escaffre et al, 2010 ; Ashash et al, 2019 ). Real time PCR was performed in 20 µL reaction mixture on Agilent Mx3000P using TaqMan chemistry (Brilliant III Ultra-Fast qRT-PCR Master Mix (Agilent Technologies, Santa Clara, CA) with 30 nM ROX as reference dye, 0.2 µM primer, and 0.25 µM TaqMan probe.…”

Section: Methodsmentioning

confidence: 99%

“…live vaccine strain ( Lazarus et al, 2008 ) has been recently developed for in ovo and DOH parenteral applications. Field studies in Latin America, Africa, and Israel have demonstrated that MB-1 induces active immunity in broiler chickens having varying maternal immunity with no adverse effects on the growth performance indices ( Ashash et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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A field study on the evaluation of day-of-hatch and in grow-out application of live infectious bursal disease virus vaccine in broiler chickens

Ray

Ashash²,

Muthukumar

2021

Poultry Science

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Infectious bursal disease ( IBD ) is an acute, highly contagious, economically important disease of young chickens caused by Avibirnavirus , the infectious bursal disease virus ( IBDV ). The causative virus is highly resilient in poultry environments and vaccination is the most effective measure for IBDV control. However, the susceptibility of highly attenuated IBDV vaccine strains to neutralization by maternally derived antibodies ( MDA ) and overwhelming virulence of partly attenuated strains have limited the application of conventional live IBDV vaccines in pre- and posthatch chicks. Nevertheless, preliminary data have raised questions about the validity of this prevailing dogma. India is an IBD endemic country and the disease causes sizeable economic losses in the sector. To evaluate the feasibility of application of live IBDV vaccine strain, the IBDV MB-1, to the maternally immunized day-of-hatch chicks in Indian production environment, 4 large-scale field trials have been conducted. The 4 trials have measured the relative safety, IBDV immunization parameters, and production performances of MB-1 vs. the established live and immune complex IBDV vaccines in a variety of commercial broiler systems. The overall health and production performances in all 4 trials have been better in the MB-1 groups. The results challenge the prevailing notion that live IBDV strains may be neutralized or break through maternal immunity and induce permanent damage to the young broiler chick's immune response. A delayed replication phenomenon following parenteral administration of the live IBDV vaccine strain has been observed, while the delayed replication mechanism remains to be elucidated. This study warrants further research on the molecular mechanism of live IBDV vaccine strain, MB-1, and its interaction with the chicken immune system.

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Co-administration of toll-like receptor (TLR)-3 agonist Poly I:C with different infectious bursal disease (IBD) vaccines improves IBD specific immune response in chicken

et al. 2021

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Toll-like receptor (TLR) agonists are emerging as promising vaccine adjuvants and immunomodulators in poultry against many diseases. Infectious bursa disease (IBD) still remains as a major threat in poultry industry. Improving the vaccine mediated immune response would help in better protection against IBD virus infection. Adjuvant potential of TLR3 agonist, Poly I:C with different IBD vaccines has been analyzed in chicken in the present study. Intermediate, intermediate plus IBD vaccine, bursaplex vaccine and their respective poly I:C combinations were used for immunization of chicken. IBD speci c antibody titre, bursa to body weight ratio, body weight gain and bursal lesion scores were evaluated at weekly interval in different immunization groups. Fold changes in cytokines IL-1β and IFN-γ mRNA expression levels were also analyzed in different groups. Intermediate IBD plus vaccine induced signi cantly (P≤0.05) higher IBD speci c antibody response at 35 days of age than other groups with comparatively lower body weight gain and moderate bursal lesion score. Poly I:C co-administration with intermediate IBD vaccine and bursaplex vaccine improved the IBD speci c antibody titres, better body weight gain and moderately less bursal lesion score. However, Poly I:C combination with intermediate plus IBD vaccine did not improve the speci c immune response. IL-1β levels were up-regulated in intermediate plus and bursaplex group, whereas IFN-γ m RNA expression levels were upregulated in intermediate IBD with Poly IC group. In conclusion, poly I:C co-administration with intermediate IBD and bursaplex vaccine was bene cial and improved the speci c immune response with least immunosuppression and bursal damage.

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In Ovo and Day of Hatch Application of a Live Infectious Bursal Disease Virus Vaccine to Commercial Broilers

Cited by 11 publications

References 27 publications

IFNγ production profile in turkeys of different immunological status after TRT vaccination

IFNγ production profile in turkeys of different immunological status after TRT vaccination

A field study on the evaluation of day-of-hatch and in grow-out application of live infectious bursal disease virus vaccine in broiler chickens

Co-administration of toll-like receptor (TLR)-3 agonist Poly I:C with different infectious bursal disease (IBD) vaccines improves IBD specific immune response in chicken

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