In planta Agrobacterium -Mediated Transformation of Rice

Ratanasut, Kumrop; Rod-In, Weerawan; Sujipuli, Kawee

doi:10.1016/j.rsci.2016.11.001

Cited by 42 publications

(29 citation statements)

References 21 publications

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“…Development and modifications of this approach greatly advanced the utilization of Arabidopsis as a model (Clough and Bent, 1998; Somerville and Koornneef, 2002; Zhang et al, 2006). Floral dip methods have also been reported for other plant species including wheat (Agarwal et al, 2009), maize (Mu et al, 2012), and rice (Ratanasut et al, 2017). However, the floral dip transformation approach for these species has not been widely adopted and they are generally transformed by Agrobacterium -mediated tissue-culture-based methods or biolistics.…”

Section: Floral Dip Transformationmentioning

confidence: 99%

The Status of Setaria viridis Transformation: Agrobacterium-Mediated to Floral Dip

Eck

2018

Front. Plant Sci.

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Setaria viridis has many attributes, including small stature and simple growth requirements, that make it attractive as a model species for monocots. Genetic engineering (transformation) methodology is a key prerequisite for adoption of plant species as models. Various transformation approaches have been reported for S. viridis including tissue culture-based and in planta by Agrobacterium tumefaciens infection of floral organs referred to as the floral dip method. The tissue culture-based method utilizes A. tumefaciens infection of mature seed-derived callus with subsequent recovery of stable transgenic lines. Vectors found to be most effective contain the hygromycin phosphotransferase selectable marker gene driven by either Panicum virgatum or Zea mays ubiquitin promoters. As for the floral dip method, there are two reports based on Agrobacterium infection of young S. viridis inflorescences. Plants were allowed to mature, seeds were collected, and analysis of the progeny verified the presence of transgenes. Each transformation approach, tissue culture-based and floral dip, has advantages and disadvantages depending on the expertise of personnel and resources available. While the tissue culture-based method results in a higher transformation efficiency than floral dip, implementation requires a specific technical skillset that limits availability of experienced personnel to successfully perform transformations. Less technical experience is required for floral dip; however, a lack of high-quality growth chambers or greenhouses that provide the necessary optimum growing conditions would reduce an already low transformation efficiency or would not result in recovery of transgenic lines. An overview of transformation methods reported for S. viridis is presented in this review.

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Section: Floral Dip Transformationmentioning

confidence: 99%

The Status of Setaria viridis Transformation: Agrobacterium-Mediated to Floral Dip

Eck

2018

Front. Plant Sci.

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“…The floral spray method can be used for in planta transformation of plant species which are too large for dipping or vacuum infiltration. A similar study on a floral dip and floral drip for rice has been reported by Ratanasut et al (2017). The transformed plants could be easily screened by a selection of the seeds on selection medium containing respective antibiotic (Harrison et al 2006 performed floral dip in planta transformation of Arabidopsis thaliana by inoculating its different floral stages with Agrobacterium containing the gus marker gene.…”

Section: Transformation Using Floral Partsmentioning

confidence: 85%

In Planta Transformation in Plants: A Review

Kaur

Devi

2019

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In planta transformation has been established as an innovative and simple technique involving direct transformation of plant parts without involving the tedious tissue culture step. Methodologies of in planta transformation involve different plant parts and strategies. Agrobacterium strain carrying the gene of interest is targeted to the specific plant part either directly or in the induction medium. Vacuum infiltration is sometimes used to facilitate foreign gene integration. Initial studies of this novel technique involved treatment of whole plants with the inoculum, and later shifted to treatments of shoot tips and floral parts and finally the female reproductive parts have been targeted. Zygotes, embryos and seeds have recently been used extensively yielding successful transformation. The method is simple, convenient and overcomes the problem of tissue culture induced genetic variability in the transformants. The review traces the origin and development in the in planta methodologies used over the past and the various parameters considered by the workers for increased effectiveness viz. developmental stages, Agrobacterium stain, surfactant, induction medium etc., in the various crops compiled. Based on the review it may be inferred that there is immense potential in planta transformation, and the ease of regeneration and selection of transformants in the methods described, can be utilized for crop improvement.

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“…2000;Barik, 2013;Narusaka et al, 2010;Zhang et al, 2006), Brassica napus (Li et al, 2010 ), orchids (Semiarti, 2012), Kalanchoe sp. (Dewanto and Suhandono, 2016), wheat (Razzaq et al, 2011), rice (Rod-in et al, 2014;Ratanasut et al, 2017), soybean (Shou et al, 2002;Zia et al, 2011) and tomatoes (Hasan et al, 2008). tumefaciens suspension so that this method is called the prick and soak method.…”

Section: A Tumefaciens-mediatedmentioning

confidence: 99%

“…The use of NPTII as a primer because of NPTII is a specific gene not owned by tomato plants (Meissner et al, 1997). In planta transformation has been successfully performed using the floral dip (Ratanasut et al, 2017;Bent 2000;Li et al, 2010;Rod-in et al, 2014;Narusaka et al, 2010;Zang et al, 2006 Clough andBent 1998), by soaking the flower of the plant in the liquid A. tumefaciens culture; the pin pricking (Razzaq et al, 2011;Jaganath et al, 2013;Seol et al, 2008), by pricking the transformation target with the needle smeared with A. tumefaciens; agro-injection (Bratic et al, 2007;Orzaez et al, 2006;Zia et al, 2011), by injecting A. tumefaciens culture directly into the plant tissue or organs; vacuum infiltration (Nanasato et al, 2013;Bratic et al, 2007), by a vacuum to force A. tumefaciens get in into plant cells;…”

Section: Molecular Analysis Of Transformant Candidates Of Tomato Plantsmentioning

confidence: 99%

PRICK AND SOAK Agroacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION IN TOMATO (Lycopersicon esculentum Mill.)

Sanjaya

Dwiyani

Wirawan

et al. 2018

Int. J. Biosci. Biotech.

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One of the modern plant breedings through genetic engineering is Agrobacterium tumefaciens-mediated transformation. Agrobacterium tumefaciens-mediated transformation can be performed in vitro or in planta. In planta transformation arises from the weaknesses of the in vitro method such as need high hygiene standard, professional tissue culture experts, and more time to prepare explants and somaclonal variation. In planta transformation is a method to transfer the gene to the plant genome without any tissue culture stages. The aims of this research were to know the possibility of the prick and soak in planta method with the target of tomato seeds and to know the most suitable inoculation time for tomato seeds transformation by prick and soak method the transformation is done by pricking the seeds and soaking them in the A. tumefaciens suspension. The treatments in this study were 1 and 2 days inoculation time to test the efficacy of prick and soak in planta transformation method. Tomato seeds were pricked with a needle on the center once, and then soaked in A. tumefaciens strain LB4404 suspension carrying pKYS-SoSPS1 plasmid with Neomycin Phosphotransferase (NPTII) and Saccharum officinarum Sucrose Phosphate synthase (SoSPS1) genes. Visualization of tomato’s DNA samples after PCR showed that 1-day inoculation sample was positively integrated with NPTII gene and negative in the 2 days inoculation treatment.

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In planta Agrobacterium -Mediated Transformation of Rice

Cited by 42 publications

References 21 publications

The Status of Setaria viridis Transformation: Agrobacterium-Mediated to Floral Dip

The Status of Setaria viridis Transformation: Agrobacterium-Mediated to Floral Dip

In Planta Transformation in Plants: A Review

PRICK AND SOAK Agroacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION IN TOMATO (Lycopersicon esculentum Mill.)

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